Current Protocols in Cytometry

Current Protocols in Cytometry

Online ISBN: 9780471142959

DOI: 10.1002/0471142956

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Cytometry
    1. Flow Cytometry
      1. A Quantitative Method for Comparing the Brightness of Antibody-dye Reagents and Estimating Antibodies Bound per Cell
      2. A Software Method for Color Compensation
      3. A Violet Ratiometric Membrane Probe for the Detection of Apoptosis
      4. Advanced Application of CFSE for Cellular Tracking
      5. Alternatives to Log-Scale Data Display
      6. Analysis of Cell Proliferation and Cell Survival by Continuous BrdU Labeling and Multivariate Flow Cytometry
      7. Analysis of DNA Content and BrdU Incorporation
      8. Analysis of DNA Content and DNA Strand Breaks for Detection of Apoptotic Cells
      9. Analysis of DNA Content and Green Fluorescent Protein Expression
      10. Analysis of DNA Denaturation
      11. Analysis of Fine-Needle Aspirate Biopsies from Solid Tumors by Laser Scanning Cytometry (LSC)
      12. Analysis of Intracellular Organelles by Flow Cytometry or Microscopy
      13. Uncompensated Polychromatic Analysis of Mitochondrial Membrane Potential Using JC-1 and Multilaser Excitation
      14. Analysis of Nuclear DNA Content and Ploidy in Higher Plants
      15. Analysis of Tissue Imprints by Scanning Laser Cytometry
      16. Analysis of Viral Infection and Viral and Cellular DNA and Proteins by Flow Cytometry
      17. Antibiotic Susceptibility Testing by Flow Cytometry
      18. Apoptosis Signaling Pathways
      19. Application of Click Chemistry Conditions for 5-Bromo-2′-Deoxyuridine Determination Through Fenton and Related Reactions
      20. Application of Flow-FISH for Dynamic Measurement of Telomere Length in Cell Division
      21. Application of the PrimRglo Assay Chemistry to Multiplexed Bead Assays
      22. Assays of Natural Killer (NK) Cell Ligation to Target Cells
      23. Assessment of Beta Cell Viability
      24. Assessment of Cell Proliferation by 5-Bromodeoxyuridine (BrdU) Labeling for Multicolor Flow Cytometry
      25. Assessment of Cell Viability
      26. Assessment of Histone Acetylation Levels in Relation to Cell Cycle Phase
      27. Assessment of Phagocyte Functions by Flow Cytometry
      28. Assessment of Surface Markers and Functionality of Dendritic Cells (DCs)
      29. Assessment of Telomere Length, Phenotype, and DNA Content
      30. Assessment of Viability, Immunofluorescence, and DNA Content
      31. Basic Preparative Techniques for Fluorescence In Situ Hybridization
      32. BeadCons: Detection of Nucleic Acid Sequences by Flow Cytometry
      33. Bivariate Analysis of DNA Content and Expression of Cyclin Proteins
      34. Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements
      35. Capture of Fluorescence Decay Times by Flow Cytometry
      36. Cell Cycle Analysis of Budding Yeast Using SYTOX Green
      37. Cell Cycle Analysis of Yeasts
      38. Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
      39. Collection, Storage, and Preparation of Human Blood Cells
      40. Comparative Overview of Flow and Image Cytometry
      41. Compensation in Flow Cytometry
      42. Critical Aspects in Analysis of Cellular DNA Content
      43. Data Analysis Through Modeling
      44. Data File Standard for Flow Cytometry, FCS 3.0
      45. Data Management
      46. Data Presentation
      47. Amine-Reactive Dyes for Dead Cell Discrimination in Fixed Samples
      48. High-Sensitivity Detection of PNH Red Blood Cells, Red Cell Precursors, and White Blood Cells
      49. Detection of Borreliacidal Antibodies by Flow Cytometry
      50. Detection of Extracellular Phosphatase Activity of Heterotrophic Prokaryotes at the Single-Cell Level by Flow Cytometry
      51. Detection of Gene Fusions in Acute Leukemia Using Bead Microarrays
      52. Detection of Mitotic Cells
      53. Determination of Bacterial Biomass from Flow Cytometric Measurements of Forward Light Scatter Intensity
      54. Dial-In Flow Cytometry Data Analysis
      55. Differential Staining of DNA and RNA
      56. Digital Data Acquisition and Processing
      57. DNA Content Measurement for DNA Ploidy and Cell Cycle Analysis
      58. DNA/RNA Analysis of Phytoplankton by Flow Cytometry
      59. DRAQ5 Labeling of Nuclear DNA in Live and Fixed Cells
      60. Dual-Pulse Labeling Using 5-Ethynyl-2′-Deoxyuridine (EdU) and 5-Bromo-2′-Deoxyuridine (BrdU) in Flow Cytometry
      61. Dynamic Thermoregulation of the Sample in Flow Cytometry
      62. Enumeration of Absolute Cell Counts Using Immunophenotypic Techniques
      63. Enumeration of CD34+ Hematopoietic Stem and Progenitor Cells
      64. Enumeration of Fetal Red Blood Cells, F Cells, and F Reticulocytes in Human Blood
      65. Enumeration of Phytoplankton, Bacteria, and Viruses in Marine Samples
      66. Enzymatic Amplification Staining for Cell Surface Antigens
      67. Establishing and Maintaining System Linearity
      68. Estimation of Membrane Potential by Flow Cytometry
      69. Estimation of Microbial Viability Using Flow Cytometry
      70. Evaluation and Purchase of an Analytical Flow Cytometer: Some of the Numerous Factors to Consider
      71. Flow Analysis and Sorting of Plant Chromosomes
      72. Flow Cytometers for Characterization of Microorganisms
      73. Flow Cytometric Analysis of Calcium Mobilization in Whole-Blood Platelets
      74. Flow Cytometric Analysis of Cell Division by Dilution of CFSE and Related Dyes
      75. Flow Cytometric Analysis of Cytokine Responses in Stimulated Whole Blood: Simultaneous Quantitation of TNF-α-Secreting Cells and Soluble Cytokines
      76. Flow Cytometric Analysis of Mitochondrial Membrane Potential Using JC-1
      77. Flow Cytometric Analysis of Reticulated Platelets
      78. Flow Cytometric Analysis of RNA Synthesis by Detection of Bromouridine Incorporation
      79. Flow Cytometric Assessment of Drug Susceptibility in Leishmania infantum Promastigotes
      80. Flow Cytometric Assessment of HLA Alloantibodies
      81. Measurement of Intracellular Ions by Flow Cytometry
      82. Flow Cytometric Detection of Pathogenic E. coli in Food
      83. Flow Cytometric Immunophenotyping of Cerebrospinal Fluid
      84. Flow Cytometric Measurement of Intracellular pH
      85. Flow Cytometric Screening for the HLA-B27 Antigen on Peripheral Blood Lymphocytes
      86. Flow Cytometry and Environmental Microbiology
      87. Flow Cytometry of Apoptosis
      88. Flow Cytometry of Extracellular Vesicles: Potential, Pitfalls, and Prospects
      89. Flow Cytometry of Murine Spermatocytes
      90. Flow Cytometry of the Side Population (SP)
      91. Flow Cytometry of Yeasts
      92. Use of Flow Cytometric Methods to Quantify Protein-Protein Interactions
      93. Flow Cytometry–Based Cytotoxicity and Antibody Binding Assay
      94. Flow Rate Calibration for Absolute Cell Counting Rationale and Design
      95. Fluidics
      96. Fluorescent Cell Barcoding for Multiplex Flow Cytometry
      97. Fountain Flow Cytometry
      98. FRET Imaging by Laser Scanning Cytometry on Large Populations of Adherent Cells
      99. Functional Assays of Oxidative Stress Using Genetically Engineered Escherichia coli Strains
      100. Handling, Storage, and Preparation of Human Tissues
      101. High-Resolution Cell Cycle and DNA Ploidy Analysis in Tissue Samples
      102. High Throughput-Based Mitochondrial Function Assays by Multi-Parametric Flow Cytometry
      103. High-Resolution Multiparameter DNA Flow Cytometry for the Detection and Sorting of Tumor and Stromal Subpopulations from Paraffin-Embedded Tissues
      104. High-Sensitivity Immunofluorescence/Flow Cytometry: Detection of Cytokine Receptors and Other Low-Abundance Membrane Molecules
      105. High-Speed Cell Sorting
      106. High-Throughput Cytotoxicity Screening by Propidium Iodide Staining
      107. Detection of Histone H2AX Phosphorylation on Ser-139 as an Indicator of DNA Damage (DNA Double-Strand Breaks)
      108. Identification of Endothelial Cells and Progenitor Cell Subsets in Human Peripheral Blood
      109. Identification of Human Antigen-Specific T Cells Using MHC Class I and Class II Tetramers
      110. Immunophenotypic Analysis of Human Mast Cells by Flow Cytometry
      111. Immunophenotypic Analysis of Peripheral Blood Lymphocytes
      112. Immunophenotypic Analysis of Platelets
      113. Immunophenotypic Analysis of PNH Cells
      114. Immunophenotypic Identification, Enumeration, and Characterization of Human Peripheral Blood Dendritic Cells and Dendritic-Cell Precursors
      115. Immunophenotyping
      116. Immunophenotyping and DNA Content Analysis of Acetone-Fixed Cells
      117. Immunophenotyping of Plasma Cells
      118. Click-iT Proliferation Assay with Improved DNA Histograms
      119. In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
      120. In Vitro Invasion Assays: Phagocytosis of the Extracellular Matrix
      121. Intracellular Cytokines
      122. Kinetic Viability Assays Using DRAQ7 Probe
      123. Labeling of Bacterial Pathogens for Flow Cytometric Detection and Enumeration
      124. Large Particle Sorting to Isolate Live Parasitic Nematode Eggs
      125. Laser Beam Shaping and Spot Size
      126. Lasers for Flow Cytometry
      127. Life Cycle Analysis of Unicellular Algae
      128. Light Scatter: Detection and Usage
      129. Listmode Data Processing
      130. Measurement and Characterization of Apoptosis by Flow Cytometry
      131. Measurement of CD40 Ligand (CD154) Expression on Resting and In Vitro–Activated T Cells
      132. Measurement of Cytogenetic Damage in Rodent Blood with a Single-Laser Flow Cytometer
      133. Measurement of Cytoplasmic to Nuclear Translocation
      134. Measurement of Low-Abundance Intracellular mRNA Using Amplified FISH Staining and Image-Based Flow Cytometry
      135. Measurement of Phagocytosis and of the Phagosomal Environment in Polymorphonuclear Phagocytes by Flow Cytometry
      136. Measurement of T Cell Activation After 16-hr In Vitro Stimulation with Concanavalin A
      137. Microsphere Surface Protein Determination Using Flow Cytometry
      138. Microsphere-Based Flow Cytometry Protease Assays for Use in Protease Activity Detection and High-Throughput Screening
      139. Use of Microsphere-Supported Phospholipid Membranes for Analysis of Protein-Lipid Interactions
      140. You have free access to this content
        Preparing a Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) Compliant Manuscript Using the International Society for Advancement of Cytometry (ISAC) FCS File Repository (
      141. Assessing Mitochondrial Redox Status by Flow Cytometric Methods: Vascular Response to Fluid Shear Stress
      142. Multidimensional Data Analysis in Immunophenotyping
      143. Multiparameter Analysis of Apoptosis Using Lab-on-a-Chip Flow Cytometry
      144. Multiparameter Analysis of Intracellular Phosphoepitopes in Immunophenotyped Cell Populations by Flow Cytometry
      145. Multiparameter Analysis of Physiological Changes in Apoptosis
      146. Multiplexed Analysis of Peptide Antigen-Specific Antibodies
      147. Multiplexed Detection of Fungal Nucleic Acid Signatures
      148. Multiplexed Microsphere-Based Flow Cytometric Immunoassays
      149. Multiplexed SNP Genotyping Using Primer Single-Base Extension (SBE) and Microsphere Arrays
      150. Multivariate Analysis
      151. Mycobacterium tuberculosis Susceptibility Testing by Flow Cytometry
      152. Nuclear DNA Content Analysis of Plant Seeds by Flow Cytometry
      153. Optical Filter Sets for Multiparameter Flow Cytometry
      154. Optimized MOL-PCR for Characterization of Microbial Pathogens
      155. Overview of Flow Cytometry and Microbiology
      156. Overview of Flow Cytometry Instrumentation
      157. Overview of Fluorescence In Situ Hybridization Techniques for Molecular Cytogenetics
      158. Overview of Functional Cell Assays
      159. Overview of Nucleic Acid Analysis
      160. Oxidative Metabolism
      161. Phenotypic Analysis Using Very Small Volumes of Blood
      162. Plug Flow Cytometry
      163. Practical Issues in High-Speed Cell Sorting
      164. Principles of Resonance Energy Transfer
      165. Probe Labeling and Fluorescence In Situ Hybridization
      166. Pulse Width for Particle Sizing
      167. Quantitative Fluorescence In Situ Hybridization (QFISH) of Telomere Lengths in Tissue and Cells
      168. Quality Control in Phenotypic Analysis by Flow Cytometry
      169. Quantification of Th1 and Th17 Cells with Intracellular Staining Following PMA/Ionomycin Stimulation
      170. Quantitative Flow Cytometric Analysis of Membrane Antigen Expression
      171. Reagents and Instruments for Multiplexed Analysis Using Microparticles
      172. Real-Time Detection of Protein Trafficking with High-Throughput Flow Cytometry (HTFC) and Fluorogen-Activating Protein (FAP) Base Biosensor
      173. Estimation of Relative Nuclear DNA Content in Dehydrated Plant Tissues by Flow Cytometry
      174. Reporters of Gene Expression: Autofluorescent Proteins
      175. Reporters of Gene Expression: Enzymatic Assays
      176. Resolution of Viable and Membrane-Compromised Free Bacteria in Aquatic Environments by Flow Cytometry
      177. RNA and DNA Aptamers in Cytomics Analysis
      178. Signal Transduction During Natural Killer Cell Activation
      179. Simultaneous Analysis of the Cyan, Green, and Yellow Fluorescent Proteins
      180. Sorting of Bacteria
      181. Spectral Flow Cytometry
      182. Sperm Chromatin Structure Assay for Fertility Assessment
      183. Standardization, Calibration, and Control in Flow Cytometry
      184. Stem Cell Side Population Analysis and Sorting Using DyeCycle Violet
      185. Stem Cell Identification and Sorting Using the Hoechst 33342 Side Population (SP)
      186. SYTO Probes: Markers of Apoptotic Cell Demise
      187. Techniques for Flow Cytometer Alignment
      188. Ten-Color Immunophenotyping of Hematopoietic Cells
      189. The Application of Data Mining to Flow Cytometry
      190. Time-Resolved Fluorescence Measurements
      191. Titration of Fluorochrome-Conjugated Antibodies for Labeling Cell Surface Markers on Live Cells
      192. Ultraviolet-Induced Detection of Halogenated Pyrimidines (UVID)
      193. Use of Spectral Fluorescence Resonance Energy Transfer to Detect Nitric Oxide-Based Signaling Events in Isolated Perfused Lung
      194. Overview of Very Small Embryonic-Like Stem Cells (VSELs) and Methodology of Their Identification and Isolation by Flow Cytometric Methods
      195. Web-Based Analysis and Publication of Flow Cytometry Experiments
      196. Whole Blood Analysis of Leukocyte-Platelet Aggregates
      197. Whole Blood Measurement of Histone Modifications Linked to the Epigenetic Regulation of Gene Expression
      198. Whole Blood Processing for Measurement of Signaling Proteins by Flow Cytometry
      199. Optimized Whole-Blood Assay for Measurement of ZAP-70 Protein Expression
      200. Yeast Cell Cycle Analysis: Combining DNA Staining with Cell and Nuclear Morphology
      201. ZAP-70 Staining in Chronic Lymphocytic Leukemia
      202. Zinc Fixation for Flow Cytometry Analysis of Intracellular and Surface Epitopes, DNA Content, and Cell Proliferation
    2. Imaging
    3. Instrumentation
    4. Microbiology
    5. Probes
    6. QC and Safety
  8. Gene Expression
  9. Genetics and Genomics
  10. Imaging and Microscopy
  11. Imaging/Microscopy
  12. Immunology
  13. Intermolecular Interactions
  14. Laboratory Organisms and Animal Models
  15. Microbiology
  16. Molecular Biology
  17. Mouse Biology
  18. Mouse Biology
  19. Neuroscience
  20. Neuroscience
  21. Nucleic Acid Chemistry
  22. Pharmacology and Drug Discovery
  23. Protein Production, Purification, and Analysis
  24. Proteomics
  25. RNA
  26. Stem Cells
  27. Supporting Lab Techniques
  28. Toxicology