Current Protocols in Cytometry

Current Protocols in Cytometry

Online ISBN: 9780471142959

DOI: 10.1002/0471142956

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Chemical Biology
  6. Cytometry
  7. Cytometry
    1. Flow Cytometry
    2. Imaging
      1. Three-Dimensional Visualization of Blood and Lymphatic Vasculature in Tissue Whole Mounts Using Confocal Microscopy
      2. 3D Deconvolution Microscopy
      3. Advanced Preparative Techniques to Establish Probes for Molecular Cytogenetics
      4. Analysis of Protein and Lipid Dynamics Using Confocal Fluorescence Recovery After Photobleaching (FRAP)
      5. Characterization of Surface FAS—Quantitative Morphological Analysis Using Quantitative Imaging Cytometry
      6. Approaches to Spectral Imaging Hardware
      7. Assessment of Cell Viability
      8. Attenuation of Replication Stress–Induced Premature Cellular Senescence to Assess Anti-Aging Modalities
      9. Automated Measurement of Blood Vessels in Tissues from Microscopy Images
      10. Basics of Digital Microscopy
      11. Flow Cytometry-Based Quantification of Cell Proliferation in the Mixed Cell Co-Culture
      12. Cell Proliferation Method: Click Chemistry Based on BrdU Coupling for Multiplex Antibody Staining
      13. Combination DNA/RNA Fish and Immunophenotyping
      14. Combined Immunofluorescence and FISH: New Prospects for Tumor Cell Detection/Identification
      15. Comparative Genomic Hybridization (CGH)—Detection of Unbalanced Genetic Aberrations Using Conventional and Micro-Array Techniques
      16. Comparative Overview of Flow and Image Cytometry
      17. Confocal Imaging of Cell Division
      18. Confocal Microscopy for High-Resolution and High-Content Analysis of the Cell Cycle
      19. Evaluation and Purchase of Confocal Microscopes: Numerous Factors to Consider
      20. Correlative Fluorescence and Electron Microscopy in 3D—Scanning Electron Microscope Perspective
      21. Cryosectioning
      22. Detecting Protein-Protein Interactions In Vivo with FRET using Multiphoton Fluorescence Lifetime Imaging Microscopy (FLIM)
      23. Detecting Protein–Protein Interactions with CFP-YFP FRET by Acceptor Photobleaching
      24. Detection and Location of Hybridization Domains on Chromosomes by Image Cytometry
      25. Dual-Pulse Labeling Using 5-Ethynyl-2′-Deoxyuridine (EdU) and 5-Bromo-2′-Deoxyuridine (BrdU) in Flow Cytometry
      26. Ex Vivo Imaging of Excised Tissue Using Vital Dyes and Confocal Microscopy
      27. Flow Cytometric FRET Analysis of ErbB Receptor Tyrosine Kinase Interaction
      28. FRET Imaging by Laser Scanning Cytometry on Large Populations of Adherent Cells
      29. From Image to Data Using Common Image-Processing Techniques
      30. From In Vitro to In Vivo: Imaging from the Single Cell to the Whole Organism
      31. High-Content Microscopy Analysis of Subcellular Structures: Assay Development and Application to Focal Adhesion Quantification
      32. High-Resolution Cytometry for High-Content Cell Cycle Analysis
      33. High-Throughput Particle Uptake Analysis by Imaging Flow Cytometry
      34. Image Processing and 2-D Morphometry
      35. Immunocytochemical Detection
      36. Immunohistochemistry
      37. Immunophenotyping
      38. Immunophenotyping Using a Laser Scanning Cytometer
      39. In Situ Proximity Ligation Assay for Microscopy and Flow Cytometry
      40. Detection of Intracellular Glutathione Using ThiolTracker Violet Stain and Fluorescence Microscopy
      41. Live Imaging of the Lung
      42. Live-Animal Imaging of Renal Function by Multiphoton Microscopy
      43. Comparative and Practical Aspects of Localization-Based Super-Resolution Imaging
      44. Measurement of Low-Abundance Intracellular mRNA Using Amplified FISH Staining and Image-Based Flow Cytometry
      45. Measurement of T-Cell Telomere Length Using Amplified-Signal FISH Staining and Flow Cytometry
      46. Measuring FRET in Flow Cytometry and Microscopy
      47. Method to Detect the Cellular Source of Over-Activated NADPH Oxidases Using NAD(P)H Fluorescence Lifetime Imaging
      48. Microscope Objectives
      49. Modern Confocal Microscopy
      50. Molecular Combing
      51. Multi-Photon Imaging
      52. Near-Infrared Molecular Probes for In Vivo Imaging
      53. Optical Filters for Wavelength Selection in Fluorescence Instrumentation
      54. Practical Methods for Molecular In Vivo Optical Imaging
      55. Principles and Applications of PRINS in Cytogenetics
      56. Processing and Staining of Cell and Tissue Material for Interphase Cytogenetics
      57. Quantitative Fluorescence In Situ Hybridization (QFISH) of Telomere Lengths in Tissue and Cells
      58. Quantitative Assessment of Pancreatic Islets Using Laser Scanning Cytometry
      59. Quantitative Fluorescent Speckle Microscopy (QFSM) to Measure Actin Dynamics
      60. Setting Up and Running an Advanced Light Microscopy and Imaging Facility
      61. Simultaneous Optical Mapping of Intracellular Free Calcium and Action Potentials from Langendorff Perfused Hearts
      62. Single-Nucleotide Sequence Discrimination In Situ Using Padlock Probes
      63. Super-Resolution Microscopy: A Comparative Treatment
      64. Three-Dimensional Image Visualization and Analysis
      65. Three-Dimensional Second-Harmonic Generation Imaging of Fibrillar Collagen in Biological Tissues
      66. Time-Lapse Microscopy Approaches to Track Cell Cycle and Lineage Progression at the Single-Cell Level
      67. Total Internal Reflection Fluorescence (TIRF) Microscopy Illuminator for Improved Imaging of Cell Surface Events
      68. Two-Dimensional Image Processing and Analysis
      69. Two-Photon Imaging of the Immune System
      70. Tyramide Signal Amplification (TSA) Systems for the Enhancement of ISH Signals in Cytogenetics
      71. Use of Spectral Fluorescence Resonance Energy Transfer to Detect Nitric Oxide-Based Signaling Events in Isolated Perfused Lung
    3. Instrumentation
    4. Microbiology
    5. Probes
    6. QC and Safety
  8. Gene Expression
  9. Genetics and Genomics
  10. Imaging and Microscopy
  11. Imaging/Microscopy
  12. Immunology
  13. Intermolecular Interactions
  14. Laboratory Organisms and Animal Models
  15. Microbiology
  16. Molecular Biology
  17. Mouse Biology
  18. Mouse Biology
  19. Neuroscience
  20. Neuroscience
  21. Nucleic Acid Chemistry
  22. Pharmacology and Drug Discovery
  23. Protein Production, Purification, and Analysis
  24. Proteomics
  25. RNA
  26. Stem Cells
  27. Supporting Lab Techniques
  28. Toxicology