Current Protocols in Cell Biology

Current Protocols in Cell Biology

Online ISBN: 9780471143031

DOI: 10.1002/0471143030

Videos


 

Current Protocols videos increase reproducibility of both basic and complex techniques of selected protocols.

UNIT 1.1 Basic Techniques in Mammalian Cell Tissue Culture

      Abstract

Video 1. Counting and Determining the Viability of Cultured Cells.
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Video 2. Freezing, Thawing, and Packaging Cells for Transport.
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Video 3. Trypsinizing and Subculturing Mammalian Cells.
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UNIT 3.30 Purification of Intact Chloroplasts from Arabidopsis and Spinach Leaves by Isopycnic Centrifugation

      Abstract

Video 1. Illustration of critical steps from the Basic Protocol.
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UNIT 4.7 Cryo-Immunogold Electron Microscopy

      Abstract

Video 1. Preparing a stainless steel loop for manipulation of grids.
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Video 2. Labeling and washing grid-mounted sections.
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Video 3. Methylcellulose embedding.
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Video 4. Fixation of cells for cryo-immunogold labeling.
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Video 5. Sucrose infiltration tissues/cells fixed for cryo-immunogold labeling.
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Video 6. Plunge freezing.
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Video 7. Preparing a mounted eyelash for handling sections.
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Video 8. Ultrathin cryo-sectioning.
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Video 9. Picking up sections after ultrathin sectioning.
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Video 10. Ultrathin section retrieval and transfer to Formvar-coated grids.
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Video 11. Coating grids with Formvar.
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UNIT 4.16 Analyzing Real-Time Video Microscopy: The Dynamics and Geometry of Vesicles and Tubules in Endocytosis

      Abstract

Video 1. Tubule formation on a vesicle during endocytosis.
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UNIT 22.9 Assembly and Micromanipulation of Xenopus In Vitro–Assembled Mitotic Chromosomes

      Abstract

Video 1. Chromosomal stretching experiment using a Xenopus in vitro–assembled mitotic chromosome (Introduction/Basic Protocol).
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Video 2. Measuring the spring constant of a micropipet (Support Protocol 6).
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Video 3. Calibration of the needle with masses (Support Protocol 7).
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