Current Protocols in Cell Biology
Online ISBN: 9780471143031
DOI: 10.1002/0471143030
Archive
Current Protocols in Cell Biology Supplements
2013: Supplement 58
2012: Supplement 57 Supplement 56 Supplement 55 Supplement 54
2011: Supplement 53 Supplement 52 Supplement 51 Supplement 50
2010: Supplement 49 Supplement 48 Supplement 47 Supplement 46
2009: Supplement 45 Supplement 44 Supplement 43 Supplement 42
2008: Supplement 41 Supplement 40 Supplement 39 Supplement 38
2007: Supplement 37 Supplement 36 Supplement 35 Supplement 34
2006: Supplement 33 Supplement 32 Supplement 31 Supplement 30
2005: Supplement 29 Supplement 28 Supplement 27 Supplement 26
2004: Supplement 25 Supplement 24 Supplement 23 Supplement 22
2003: Supplement 21 Supplement 20 Supplement 19 Supplement 18
Supplement 17
2002: Supplement 16 Supplement 15 Supplement 14 Supplement 13
2001: Supplement 12 Supplement 11 Supplement 10 Supplement 9
2000: Supplement 8 Supplement 7 Supplement 6 Supplement 5
1999: Supplement 4 Supplement 3 Supplement 2 Supplement 1
1998: Core publication
Supplement 58, March 2013
Unit 4.21 Photoactivated Localization Microscopy (PALM) of Adhesion Complexes
Abstract | Full Text: HTML PDF
Unit 17.14 Chromatin Immunoprecipitation of Adult Murine Cardiomyocytes
Abstract | Full Text: HTML PDF
Unit 19.7 Imaging Tumor Cell Movement In Vivo
Abstract | Full Text: HTML PDF
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Supplement 57, December 2012
Unit 4.26 Visualization of Live Primary Cilia Dynamics Using Fluorescence Microscopy
Abstract | Full Text: HTML PDF
Unit 10.1 Overview of Extracellular Matrix
Abstract | Full Text: HTML PDF
Unit 15.18 Analysis of Rab GTPases
Abstract | Full Text: HTML PDF
Unit 15.19 Synchronizing Protein Transport in the Secretory Pathway
Abstract | Full Text: HTML PDF
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Supplement 56, September 2012
Unit 7.11 Metabolic Labeling with Noncanonical Amino Acids and Visualization by Chemoselective Fluorescent Tagging
Abstract | Full Text: HTML PDF
Unit 19.15 Chick Embryo Culture and Electroporation
Abstract | Full Text: HTML PDF
Unit 22.15 Analysis of Interactions Between Genomic Loci Through Chromosome Conformation Capture (3C)
Abstract | Full Text: HTML PDF
Unit 22.16 Assays to Measure Nuclear Mechanics in Interphase Cells
Abstract | Full Text: HTML PDF
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Supplement 55, June 2012
Unit 8.8 Analysis of Protein Turnover by Quantitative SNAP-Based Pulse-Chase Imaging
Abstract | Full Text: HTML PDF
Unit 17.13 Approaches to Studying Arf GAPs in Cells: In Vitro Assay with Isolated Focal Adhesions
Abstract | Full Text: HTML PDF
Unit 23.8 Cardiac Differentiation of Human Embryonic Stem Cells and their Assembly into Engineered Heart Muscle
Abstract | Full Text: HTML PDF
Unit 27.4 Analysis of Nonsense-Mediated mRNA Decay in Mammalian Cells
Abstract | Full Text: HTML PDF
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Supplement 54, March 2012
Unit 15.16 Measurement of Autophagic Activity in Mammalian Cells
Abstract | Full Text: HTML PDF
Unit 15.17 Analysis of Polarized Membrane Traffic in Hepatocytes and Hepatic Cell Lines
Abstract | Full Text: HTML PDF
Unit 27.3 Analysis of Nonsense-Mediated mRNA Decay in Saccharomyces cerevisiae
Abstract | Full Text: HTML PDF
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Supplement 53, December 2011
Unit 9.4 Analyzing Integrin-Dependent Adhesion
Abstract | Full Text: HTML PDF
Unit 14.13 Allosteric Activation of Kinases: Design and Application of RapR Kinases
Abstract | Full Text: HTML PDF
Unit 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Abstract | Full Text: HTML PDF
Unit 26.14 Methods for Growing and Titrating African Swine Fever Virus: Field and Laboratory Samples
Abstract | Full Text: HTML PDF
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Supplement 52, September 2011
Unit 4.19 Quantitative Colocalization Analysis of Confocal Fluorescence Microscopy Images
Abstract | Full Text: HTML PDF
Unit 6.2 Immunoblotting and Immunodetection
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Unit 20.5 Transfection by Electroporation
Abstract | Full Text: HTML PDF
Unit 26.13 A Dual-Chamber Model of the Female Genital Tract to Evaluate Epithelial Toxicity of Candidate Anti-HIV Microbicides
Abstract | Full Text: HTML PDF
Appendix 3H The Colorimetric Detection and Quantitation of Total Protein
Abstract | Full Text: HTML PDF (238K)
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Supplement 51, June 2011
Unit 17.12 Use of In Vivo Biotinylation for Chromatin Immunoprecipitation
Abstract | Full Text: HTML PDF
Unit 26.10 Evaluation of Antiseptic Antiviral Activity of Chemical Agents
Abstract | Full Text: HTML PDF
Unit 26.11 Rapid Titration of Viruses by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 26.12 An Enzymatic Assay for Detection of Viral Entry
Abstract | Full Text: HTML PDF
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Supplement 50, March 2011
Unit 8.7 Cell Biological Analysis of DT40 Knockout Cell Lines for Cell-Cycle Genes
Abstract | Full Text: HTML PDF
Unit 26.7 COS-1 Cells as Packaging Host for Production of Lentiviruses
Abstract | Full Text: HTML PDF
Unit 26.8 Production of Lentiviral Vectors in Protein-Free Media
Abstract | Full Text: HTML PDF
Unit 26.9 Monitoring Viral-Mediated Membrane Fusion Using Fluorescent Reporter Methods
Abstract | Full Text: HTML PDF
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Supplement 49, December 2010
Unit 3.40 Purification of Ribosomes from Human Cell Lines
Abstract | Full Text: HTML PDF
Unit 5.7 Determining Membrane Protein Topologies in Single Cells and High-Throughput Screening Applications
Abstract | Full Text: HTML PDF
Unit 6.11 Measurement of Oxidatively-Induced Clustered DNA Lesions Using a Novel Adaptation of Single Cell Gel Electrophoresis (Comet Assay)
Abstract | Full Text: HTML PDF
Unit 22.14 Analysis of DNA Replication in Saccharomyces cerevisiae by Two-Dimensional and Pulsed-Field Gel Electrophoresis
Abstract | Full Text: HTML PDF
Unit 27.2 Gene Down-Regulation with Short Hairpin RNAs and Validation of Specificity by Inducible Rescue in Mammalian Cells
Abstract | Full Text: HTML PDF
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Supplement 48, September 2010
Unit 3.38 Isolation of Aggresomes and Other Large Aggregates
Abstract | Full Text: HTML PDF
Unit 3.39 Isolation of Chromaffin Granules
Abstract | Full Text: HTML PDF
Unit 8.6 Dynamic Proliferation Assessment in Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 10.18 Imaging Cells in Three-Dimensional Collagen Matrix
Abstract | Full Text: HTML PDF
Unit 14.12 Analysis of Arf GTP-Binding Protein Function in Cells
Abstract | Full Text: HTML PDF
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Supplement 47, June 2010
Unit 3.36 Isolation of Nucleoli
Abstract | Full Text: HTML PDF
Unit 3.37 Isolation of Cytotoxic T Cell and NK Granules and Purification of Their Effector Proteins
Abstract | Full Text: HTML PDF
Unit 10.16 Preparation of Hydrogel Substrates with Tunable Mechanical Properties
Abstract | Full Text: HTML PDF
Unit 10.17 Engineering Three-Dimensional Collagen Matrices to Provide Contact Guidance during 3D Cell Migration
Abstract | Full Text: HTML PDF
Unit 18.10 High-Throughput Live Cell Imaging of Apoptosis
Abstract | Full Text: HTML PDF
Unit 27.1 Silencing of Gene Expression in Cultured Cells Using Small Interfering RNAs
Abstract | Full Text: HTML PDF
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Supplement 46, March 2010
Unit 3.34 Isolation of Legionella-Containing Vacuoles by Immuno-Magnetic Separation
Abstract | Full Text: HTML PDF
Unit 3.35 Isolation of Platelet Granules
Abstract | Full Text: HTML PDF
Unit 4.25 Analysis of Mitochondrial Dynamics and Functions Using Imaging Approaches
Abstract | Full Text: HTML PDF
Unit 14.11 Biosensors for Characterizing the Dynamics of Rho Family GTPases in Living Cells
Abstract | Full Text: HTML PDF
Unit 15.14 Quantitative Analysis of Endocytosis and Turnover of Epidermal Growth Factor (EGF) and EGF Receptor
Abstract | Full Text: HTML PDF
Unit 15.15 Documenting GLUT4 Exocytosis and Endocytosis in Muscle Cell Monolayers
Abstract | Full Text: HTML PDF
Unit 26.6 Methods for Monitoring Dynamics of Pulmonary RSV Replication by Viral Culture and by Real-Time Reverse Transcription–PCR In Vivo: Detection of Abortive Viral Replication
Abstract | Full Text: HTML PDF
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Supplement 45, December 2009
Unit 4.23 Interference Reflection Microscopy
Abstract | Full Text: HTML PDF
Unit 4.24 Fluorescence Correlation Spectroscopy in Living Cells: A Practical Approach
Abstract | Full Text: HTML PDF
Unit 10.15 Generation of Micropatterned Substrates Using Micro Photopatterning
Abstract | Full Text: HTML PDF
Unit 14.10 In Vivo Imaging of Signal Transduction Cascades with Probes Based on Förster Resonance Energy Transfer (FRET)
Abstract | Full Text: HTML PDF
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Supplement 44, September 2009
Unit 3.33 Isolation and Biochemical Characterization of Amyloid Plaques and Paired Helical Filaments
Abstract | Full Text: HTML PDF
Unit 19.12 Overview: Generation of Gene Knockout Mices
Abstract | Full Text: HTML PDF
Unit 19.13 Manipulation of Mouse Embryonic Stem Cells for Knockout Mouse Production
Abstract | Full Text: HTML PDF
Unit 19.14 Generation of Gene Knockout Mice by ES Cell Microinjection
Abstract | Full Text: HTML PDF
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Supplement 43, June 2009
Unit 4.22 Culturing MDCK Cells in Three Dimensions for Analyzing Intracellular Dynamics
Abstract | Full Text: HTML PDF
Unit 14.9 In Vitro GEF and GAP Assays
Abstract | Full Text: HTML PDF
Unit 26.3 Methods Used to Study Respiratory Virus Infection
Abstract | Full Text: HTML PDF
Unit 26.4 Compartmented Neuron Cultures for Directional Infection by Alpha Herpesviruses
Abstract | Full Text: HTML PDF
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Supplement 42, March 2009
Unit 3.32 Isolation of Dense Core Secretory Vesicles from Pancreatic Endocrine Cells by Differential and Density Gradient Centrifugation
Abstract | Full Text: HTML PDF
Unit 19.10 Overview: Engineering Transgenic Constructs and Mice
Abstract | Full Text: HTML PDF
Unit 19.11 Generation of Transgenic Mice
Abstract | Full Text: HTML PDF
Unit 24.4 Visualization of Cellular Phosphoinositide Pools with GFP-Fused Protein-Domains
Abstract | Full Text: HTML PDF
Abstract | Full Text: HTML PDF
Unit 26.2 BK Virus (BKV): Infection, Propagation, Quantitation, Purification, Labeling, and Analysis of Cell Entry
Abstract | Full Text: HTML PDF
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Supplement 41, December 2008
Unit 3.31 Isolation of Neuromelanin Granules
Abstract | Full Text: HTML PDF
Unit 4.21 Photoactivated Localization Microscopy (PALM) of Adhesion Complexes
Abstract | Full Text: HTML PDF
Unit 19.8 Embryonic Organ Culture
Abstract | Full Text: HTML PDF
Unit 19.9 Three-Dimensional Tissue Models of Normal and Diseased Skin
Abstract | Full Text: HTML PDF
Unit 22.13 Monitoring mRNA Export
Abstract | Full Text: HTML PDF
Supplement 40, September 2008
Unit 3.30 Purification of Intact Chloroplasts from Arabidopsis and Spinach Leaves by Isopycnic Centrifugation
Abstract | Full Text: HTML PDF
Unit 10.8 Matrix Metalloproteinases
Abstract | Full Text: HTML PDF
Unit 10.14 Use of Hyaluronan-Derived Hydrogels for Three-Dimensional Cell Culture and Tumor Xenografts
Abstract | Full Text: HTML PDF
Unit 15.13 Analysis of Endocytic Trafficking by Single-Cell Fluorescence Ratio Imaging
Abstract | Full Text: HTML PDF
Unit 24.3 Making Giant Unilamellar Vesicles via Hydration of a Lipid Film
Abstract | Full Text: HTML PDF
Supplement 39, June 2008
Unit 3.29 Isolation of Microtubules and Microtubule Proteins
Abstract | Full Text: HTML PDF
Unit 4.18 Fluorescence Imaging Techniques for Studying Drosophila Embryo Development
Abstract | Full Text: HTML PDF
Unit 4.19 Quantitative Colocalization Analysis of Confocal Fluorescence Microscopy Images
Abstract | Full Text: HTML PDF
Unit 4.20 Visualizing Protease Activity in Living Cells: From Two Dimensions to Four Dimensions
Abstract | Full Text: HTML PDF
Unit 22.11 Assays for Ribosomal RNA Processing and Ribosome Assembly
Abstract | Full Text: HTML PDF
Unit 22.12 Visualization and Measurement of DNA Methyltransferase Activity in Living Cells
Abstract | Full Text: HTML PDF
Supplement 38, March 2008
Unit 3.28 Isolation of Amyloplasts
Abstract | Full Text: HTML PDF (311K)
Unit 6.10 Two-Dimensional Blue Native Polyacrylamide Gel Electrophoresis
Abstract | Full Text: HTML PDF (301K)
Unit 14.8 Rho GTPase Activation Assays
Abstract | Full Text: HTML PDF (319K)
Unit 21.6 Photoactivation and Imaging of Photoactivatable Fluorescent Proteins
Abstract | Full Text: HTML PDF (445K)
Supplement 37, December 2007
Unit 3.27 Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts
Abstract | Full Text: HTML PDF (655K)
Unit 4.17 Scanning Electron Microscopy of Cell Surface Morphology
Abstract | Full Text: HTML PDF (404K)
Unit 6.1 One-Dimensional SDS Gel Electrophoresis of Proteins
Abstract | Full Text: HTML PDF (891K)
Unit 17.1 Visualization of RNA Using Fluorescence Complementation Triggered byAptamer-Protein Interactions (RFAP) in Live Bacterial Cells
Abstract | Full Text: HTML PDF (466K)
Unit 26.1 Production of Papillomavirus-Based Gene Transfer Vectors
Abstract | Full Text: HTML PDF (294K)
Supplement 36, September 2007
Unit 1.1 Basic Techniques in Mammalian Cell Tissue Culture
Abstract | Full Text: HTML PDF (205K)
Unit 15.12 Analysis of Regulated Secretion Using PC12 Cells
Abstract | Full Text: HTML PDF (329K)
Unit 23.6 Hematoendothelial Differentiation of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF (507K)
Unit 23.7 Neural Differentiation of Human ES Cells
Abstract | Full Text: HTML PDF (249K)
Unit 25.1 In Vivo Imaging Using Quantum Dot–Conjugated Probes
Abstract | Full Text: HTML PDF (409K)
Supplement 35, June 2007
Unit 4.16 Analyzing Real-Time Video Microscopy: The Dynamics and Geometry of Vesicles and Tubules in Endocytosis
Abstract | Full Text: HTML PDF (378K)
Unit 19.7 Imaging Tumor Cell Movement In Vivo
Abstract | Full Text: HTML PDF (316K)
Unit 22.10 Replication Labeling with Halogenated Thymidine Analogs
Abstract | Full Text: HTML PDF (197K)
Unit 24.1 Using Fluorescent Sphingolipid Analogs to Study Intracellular Lipid Trafficking
Abstract | Full Text: HTML PDF (725K)
Unit 24.2 Fluorescent Detection of Lipid Droplets and Associated Proteins
Abstract | Full Text: HTML PDF (174K)
Supplement 34, March 2007
Unit 3.26 Isolation of Renal Brush Borders
Abstract | Full Text: HTML PDF (610K)
Unit 4.15 Biological Second and Third Harmonic Generation Microscopy
Abstract | Full Text: HTML PDF (1435K)
Unit 11.19 In Vitro Analysis of Yeast Mitochondrial Protein Import
Abstract | Full Text: HTML PDF (154K)
Unit 23.3 Maintenance and In Vitro Differentiation of Mouse Embryonic Stem Cells to Form Blood Vessels
Abstract | Full Text: HTML PDF (350K)
Unit 23.4 Differentiation of Mouse Embryonic Stem Cells and of Human Adult Stem Cells into Adipocytes
Abstract | Full Text: HTML PDF (422K)
Unit 23.5 Induction of ES Cell–Derived Cartilage Formation
Abstract | Full Text: HTML PDF (684K)
Supplement 33, December 2006
Unit 3.25 Isolation of Myelin
Abstract | Full Text: HTML PDF (437K)
Unit 10.9 Preparation of Extracellular Matrices Produced by Cultured Fibroblasts
Abstract | Full Text: HTML PDF (389K)
Unit 17.10 Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
Abstract | Full Text: HTML PDF (891K)
Unit 21.5 The Fluorescent Protein Color Palette
Abstract | Full Text: HTML PDF (1079K)
Supplement 32, September 2006
Unit 2.7 Preparation and Coculture of Neurons and Glial Cells
Abstract | Full Text: HTML PDF (212K)
Unit 15.10 Measuring Retrograde Transport to the Trans -Golgi Network
Abstract | Full Text: HTML PDF (334K)
Unit 15.11 Assays for Regulated Exocytosis of Mast Cell Granules
Abstract | Full Text: HTML PDF (159K)
Unit 17.9 Rational Design and Evaluation of FRET Experiments to Measure Protein Proximities in Cells
Abstract | Full Text: HTML PDF (844K)
Supplement 31, June 2006
Unit 3.23 Isolation of Intermediate Filaments
Abstract | Full Text: HTML PDF (161K)
Unit 3.24 Isolation of T-Tubules from Skeletal Muscle
Abstract | Full Text: HTML PDF (496K)
Unit 15.9 Studies of the Ubiquitin Proteasome System
Abstract | Full Text: HTML PDF (622K)
Supplement 30, March 2006
Unit 3.20 Isolation of GLUT4 Storage Vesicles
Abstract | Full Text: HTML PDF (264K)
Unit 3.21 Isolation of Intestinal Brush-Border Membranes
Abstract | Full Text: HTML PDF (402K)
Unit 3.22 Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
Abstract | Full Text: HTML PDF (260K)
Unit 4.7 Cryo-Immunogold Electron Microscopy
Abstract | Full Text: HTML PDF (2023K)
Unit 14.7 Analyzing FAK and Pyk2 in Early Integrin Signaling Events
Abstract | Full Text: HTML PDF (330K)
Supplement 29, December 2005
Unit 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation
Abstract | Full Text: HTML PDF (257K)
Unit 3.16 Isolation of Mast Cell Granules
Abstract | Full Text: HTML PDF (472K)
Unit 3.17 Immunoisolation of Centrosomes from Drosophila melanogaster
Abstract | Full Text: HTML PDF (272K)
Unit 3.18 Isolation of Zymogen Granules from Rat Pancreas
Abstract | Full Text: HTML PDF (509K)
Unit 3.19 Isolation of Glyoxysomes from Pumpkin Cotyledons
Abstract | Full Text: HTML PDF (313K)
Unit 11.11 Analysis of the Cell Cycle Using Xenopus Egg Extracts
Abstract | Full Text: HTML PDF (163K)
Unit 21.3 Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation ( B i FC ) Analysis
Abstract | Full Text: HTML PDF (471K)
Supplement 28, September 2005
Unit 12.8 In Vivo Marking of Single Cells in Chick Embryos Using Photoactivation of GFP
Abstract | Full Text: HTML PDF (416K)
Unit 14.3 The Detection of MAPK Signaling
Abstract | Full Text: HTML PDF (445K)
Unit 23.1 Stem Cells: An Overview
Abstract | Full Text: HTML PDF (488K)
Unit 23.2 Mouse Embryonic Stem Cell Derivation, and Mouse and Human Embryonic Stem Cell Culture and Differentiation as Embryoid Bodies
Abstract | Full Text: HTML PDF (366K)
Supplement 27, June 2005
Unit 11.18 Endocytosis Assays in Intact and Permeabilized Cells
Abstract | Full Text: HTML PDF (238K)
Unit 15.8 Analysis of Protein Transport to Lysosomes
Abstract | Full Text: HTML PDF (156K)
Unit 18.9 Analysis of Cellular Senescence in Culture In Vivo: The Senescence-Associated -Galactosidase Assay
Abstract | Full Text: HTML PDF (124K)
Unit 20.8 Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
Abstract | Full Text: HTML PDF (143K)
Unit 21.4 Design and Use of Fluorescent Fusion Proteins in Cell Biology
Abstract | Full Text: HTML PDF (234K)
Supplement 26, March 2005
Unit 3.13 Isolation of Clathrin-Coated Vesicles by Differential and Density Gradient Centrifugation
Abstract | Full Text: HTML PDF (1019K)
Unit 3.14 Isolation of Melanosomes
Abstract | Full Text: HTML PDF (773K)
Unit 13.6 Functional Characterization of Proteins Regulating Actin Assembly
Abstract | Full Text: HTML PDF (301K)
Unit 21.3 Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation ( B i FC ) Analysis
Abstract | Full Text: HTML PDF (471K)
Unit 22.9 Assembly and Micromanipulation of Xenopus In Vitro–Assembled Mitotic Chromosomes
Abstract | Full Text: HTML PDF (383K)
Supplement 25, December 2004
Unit 3.12 Isolation of Synaptic Vesicles
Abstract | Full Text: HTML PDF (372K)
Unit 4.14 Fluorescence Lifetime Imaging Microscopy
Abstract | Full Text: HTML PDF (653K)
Unit 10.12 Analysis of Fibronectin Matrix Assembly
Abstract | Full Text: HTML PDF (77K)
Unit 10.13 Non-Radioactive Quantification of Fibronectin Matrix Assembly
Abstract | Full Text: HTML PDF (180K)
Unit 22.6 Comparative Genomic Hybridization
Abstract | Full Text: HTML PDF (2824K)
Unit 22.7 Sister Chromatid Exchange
Abstract | Full Text: HTML PDF (227K)
Unit 22.8 Detection of Mitotic Figures and Components of the Mitotic Machinery
Abstract | Full Text: HTML PDF (107K)
Supplement 24, September 2004
Unit 12.7 Actin-Based Motility Assay
Abstract | Full Text: HTML PDF (260K)
Unit 21.2 Fluorescence Localization After Photobleaching ( FLAP )
Abstract | Full Text: HTML PDF (896K)
Unit 22.5 Multi-Color FISH Techniques
Abstract | Full Text: HTML PDF (731K)
Supplement 23, June 2004
Unit 17.7 Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
Abstract | Full Text: HTML PDF (241K)
Unit 17.8 Isothermal Titration Calorimetry
Abstract | Full Text: HTML PDF (486K)
Unit 22.1 Overview of Cytogenetic Chromosome Analysis
Abstract | Full Text: HTML PDF (104K)
Unit 22.2 Preparation of Cytogenetic Specimens from Tissue Samples
Abstract | Full Text: HTML PDF (186K)
Unit 22.3 Traditional Banding of Chromosomes for Cytogenetic Analysis
Abstract | Full Text: HTML PDF (453K)
Unit 22.4 Fluorescence In Situ Hybridization ( FISH )
Abstract | Full Text: HTML PDF (709K)
Supplement 22, March 2004
Unit 14.6 Determination of Akt/ PKB Signaling
Abstract | Full Text: HTML PDF (272K)
Unit 15.7 Measurements of Phagocytosis and Phagosomal Maturation
Abstract | Full Text: HTML PDF (384K)
Unit 17.5 Protein-Protein Interactions Identified by Pull-Down Experiments and Mass Spectrometry
Abstract | Full Text: HTML PDF (679K)
Unit 17.6 Measuring Protein Interactions by Optical Biosensors
Abstract | Full Text: HTML PDF (356K)
Supplement 21, December 2003
Unit 2.6 Preparation of Human Epidermal Keratinocyte Cultures
Abstract | Full Text: HTML PDF (261K)
Unit 18.7 Nonisotopic Methods for Determination of Poly( ADP -Ribose) Levels and Detection of Poly( ADP -Ribose) Polymerase
Abstract | Full Text: HTML PDF (718K)
Unit 18.8 Flow Cytometry of Apoptosis
Abstract | Full Text: HTML PDF (531K)
Unit 19.6 Experimental Metastasis Assays in the Chick Embryo
Abstract | Full Text: HTML PDF (568K)
Supplement 20, September 2003
Unit 4.11 Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues
Abstract | Full Text: HTML PDF (1421K)
Unit 4.12 Total Internal Reflection Fluorescence Microscopy for High-Resolution Imaging of Cell-Surface Events
Abstract | Full Text: HTML PDF (431K)
Unit 4.13 Fluorescent Labeling of Yeast
Abstract | Full Text: HTML PDF (525K)
Unit 18.6 Analysis of Telomeres and Telomerase
Abstract | Full Text: HTML PDF (647K)
Supplement 19, June 2003
Unit 1.7 BY-2 Cells: Culture and Transformation for Live Cell Imaging
Abstract | Full Text: HTML PDF (332K)
Unit 18.5 Analysis of Mitochondrial Dysfunction During Cell Death
Abstract | Full Text: HTML PDF (360K)
Unit 20.3 Calcium Phosphate Transfection
Abstract | Full Text: HTML PDF (185K)
Unit 20.4 Transfection Using DEAE -Dextran
Abstract | Full Text: HTML PDF (200K)
Unit 20.5 Transfection by Electroporation
Abstract | Full Text: HTML PDF (148K)
Unit 20.6 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
Abstract | Full Text: HTML PDF (409K)
Unit 20.7 Optimization of Transfection
Abstract | Full Text: HTML PDF (150K)
Supplement 18, March 2003
Unit 9.6 Measurement of Adhesion Under Flow Conditions
Abstract | Full Text: HTML PDF (241K)
Unit 11.17 In Vitro RNA Splicing in Mammalian Cell Extracts
Abstract | Full Text: HTML PDF (315K)
Unit 13.5 Measuring Dynamics of Nuclear Proteins by Photobleaching
Abstract | Full Text: HTML PDF (249K)
Unit 19.5 The Chick Chorioallantoic Membrane as an In Vivo Angiogenesis Model
Abstract | Full Text: HTML PDF (219K)
Unit 20.1 Direct Introduction of Molecules into Cells
Abstract | Full Text: HTML PDF (150K)
Unit 20.2 Protein Transduction: Generation of Full-Length Transducible Proteins Using the TAT System
Abstract | Full Text: HTML PDF (544K)
Supplement 17, January 2003
Unit 5.6 Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry
Abstract | Full Text: HTML PDF (308K)
Unit 10.10 Purification and Analysis of Thrombospondin-1
Abstract | Full Text: HTML PDF (224K)
Unit 10.11 Purification of SPARC /Osteonectin
Abstract | Full Text: HTML PDF (266K)
Unit 12.6 Optical Microscopy–Based Migration Assay for Human Neutrophils
Abstract | Full Text: HTML PDF (548K)
Unit 17.4 Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries
Abstract | Full Text: HTML PDF (196K)
Supplement 16, October 2002
Unit 6.8 Fluorescence Detection of Glycoproteins in Gels and on Electroblots
Abstract | Full Text: HTML PDF (218K)
Unit 6.9 Digital Electrophoresis Analysis
Abstract | Full Text: HTML PDF (263K)
Unit 10.9 Preparation of Extracellular Matrices Produced by Cultured Fibroblasts
Unit 19.3 Microanalysis of Gene Expression in Tissues Using T7-SAGE: Serial Analysis of Gene Expression After High-Fidelity T7-Based RNA Amplification
Abstract | Full Text: HTML PDF (350K)
Unit 19.4 SAGE Analysis from 1 µg of Total RNA
Abstract | Full Text: HTML PDF (263K)
Supplement 15, July 2002
Unit 6.7 Agarose Gel Electrophoresis of Proteins
Abstract | Full Text: HTML PDF (187K)
Unit 7.10 Radioiodination of Cellular Proteins
Abstract | Full Text: HTML PDF (157K)
Unit 9.4 Analyzing Integrin-Dependent Adhesion
Abstract | Full Text: HTML PDF (274K)
Appendix 3B Spectrophotometric Determination of Protein Concentration
Abstract | Full Text: HTML PDF (86K)
Appendix 3H The Colorimetric Detection and Quantitation of Total Protein
Abstract | Full Text: HTML PDF (238K)
Supplement 14, April 2002
Unit 3.11 Free-Flow Electrophoretic Analysis of Endosome Subpopulations of Rat Hepatocytes
Abstract | Full Text: HTML PDF (391K)
Unit 7.9 Analysis of Oxidative Modification of Proteins
Abstract | Full Text: HTML PDF (239K)
Unit 11.15 In Vitro Analysis of Peroxisomal Protein Import
Abstract | Full Text: HTML PDF (178K)
Unit 11.16 In Vitro Analysis of Chloroplast Protein Import
Abstract | Full Text: HTML PDF (234K)
Unit 15.6 Analysis of Protein Folding and Oxidation in the Endoplasmic Reticulum
Abstract | Full Text: HTML PDF (375K)
Supplement 13, January 2002
Unit 4.9 Polarization Microscopy
Abstract | Full Text: HTML PDF (1367K)
Unit 4.10 Fluorescent Speckle Microscopy ( FSM ) of Microtubules and Actin in Living Cells
Abstract | Full Text: HTML PDF (563K)
Unit 7.8 Metabolic Labeling of Glycoproteins with Radioactive Sugars
Abstract | Full Text: HTML PDF (150K)
Unit 10.8 Matrix Metalloproteinases
Full Text: HTML PDF (836K)
Unit 16.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides
Abstract | Full Text: HTML PDF (206K)
Supplement 12, October 2001
Unit 3.10 Isolation of Nuclei and Nuclear Membranes From Animal Tissues
Abstract | Full Text: HTML PDF (174K)
Unit 7.7 Metabolic Labeling and Immunoprecipitation of Drosophila Proteins
Abstract | Full Text: HTML PDF (92K)
Unit 15.5 Analysis of Membrane Traffic in Polarized Epithelial Cells
Abstract | Full Text: HTML PDF (202K)
Unit 18.3 Assessment of Apoptosis and Necrosis by DNA Fragmentation and Morphological Criteria
Abstract | Full Text: HTML PDF (358K)
Unit 18.4 Quantitative Fluorescence In Situ Hybridization (Q-FISH)
Abstract | Full Text: HTML PDF (405K)
Unit 19.1 Overview of Metastasis Assays
Abstract | Full Text: HTML PDF (218K)
Unit 19.2 Tail Vein Assay of Cancer Metastasis
Abstract | Full Text: HTML PDF (186K)
Supplement 11, July 2001
Unit 4.8 Correlative Video Light/Electron Microscopy
Abstract | Full Text: HTML PDF (408K)
Unit 9.5 Analysis of Cell-Cell Contact Mediated by Ig Superfamily Cell Adhesion Molecules
Abstract | Full Text: HTML PDF (820K)
Unit 18.1 Current Concepts in Cell Death
Abstract | Full Text: HTML PDF (306K)
Unit 18.2 Analysis of Caspase Activation During Apoptosis
Abstract | Full Text: HTML PDF (380K)
Supplement 10, April 2001
Unit 2.5 Laser Capture Microdissection
Abstract | Full Text: HTML PDF (299K)
Unit 3.9 Isolation of Golgi Membranes from Tissues and Cells by Differential and Density Gradient Centrifugation
Abstract | Full Text: HTML PDF (221K)
Unit 11.14 Analysis of RNA Export Using Xenopus Oocytes
Abstract | Full Text: HTML PDF (208K)
Unit 13.4 Movement of Nuclei
Abstract | Full Text: HTML PDF (450K)
Appendix 3F Enzymatic Amplification of DNA by PCR : Standard Procedures and Optimization
Abstract | Full Text: HTML PDF (153K)
Appendix 3G Micro RT-PCR
Abstract | Full Text: HTML PDF (73K)
Supplement 9, January 2001
Unit 11.10 Preparation and Use of Interphase Xenopus Egg Extracts
Abstract | Full Text: HTML PDF (333K)
Unit 11.11 Analysis of the Cell Cycle Using Xenopus Egg Extracts
Unit 11.12 Analysis of Apoptosis Using Xenopus Egg Extracts
Abstract | Full Text: HTML PDF (155K)
Unit 11.13 Mitotic Spindle Assembly In Vitro
Abstract | Full Text: HTML PDF (330K)
Unit 12.5 Dictyostelium Cell Dynamics
Abstract | Full Text: HTML PDF (630K)
Unit 13.3 Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP
Abstract | Full Text: HTML PDF (394K)
Supplement 8, October 2000
Unit 3.8 Isolation of Subcellular Fractions from the Yeast Saccharomyces cerevisiae
Abstract | Full Text: HTML PDF (504K)
Unit 11.9 Analysis of Eukaryotic Translation in Purified and Semipurified Systems
Abstract | Full Text: HTML PDF (293K)
Unit 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
Abstract | Full Text: HTML PDF (464K)
Appendix 3E Silanizing Glassware
Abstract | Full Text: HTML PDF (35K)
Supplement 7, July 2000
Unit 3.6 Isolation of Lysosomes from Tissues and Cells by Differential and Density Gradient Centrifugation
Abstract | Full Text: HTML PDF (215K)
Unit 3.7 Overview of Subcellular Fractionation Procedures for the Yeast Saccharomyces cerevisiae
Abstract | Full Text: HTML PDF (273K)
Unit 10.7 Proteoglycan Isolation and Analysis
Abstract | Full Text: HTML PDF (166K)
Unit 17.1 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer ( FRET ) Microscopy
Abstract | Full Text: HTML PDF (343K)
Unit 17.2 Identification of Protein Interactions by Far Western Analysis
Abstract | Full Text: HTML PDF (196K)
Appendix 1G Centrifuges and Rotors
Abstract | Full Text: HTML PDF (84K)
Appendix 3D Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy
Abstract | Full Text: HTML PDF (162K)
Supplement 6, April 2000
Unit 3.5 Isolation of Peroxisomes from Tissues and Cells by Differential and Density Gradient Centrifugation
Abstract | Full Text: HTML PDF (206K)
Unit 5.5 Determination of Molecular Size by Size-Exclusion Chromatography (Gel Filtration)
Abstract | Full Text: HTML PDF (234K)
Unit 6.6 Staining Proteins in Gels
Abstract | Full Text: HTML PDF (210K)
Unit 7.6 Metabolic Labeling and Immunoprecipitation of Yeast Proteins
Abstract | Full Text: HTML PDF (111K)
Unit 11.8 In Vitro Translation Using He L a Extract
Abstract | Full Text: HTML PDF (144K)
Unit 16.5 Antibody Conjugates for Cell Biology
Abstract | Full Text: HTML PDF (253K)
Supplement 5, January 2000
Unit 6.5 One-Dimensional Electrophoresis Using Nondenaturing Conditions
Abstract | Full Text: HTML PDF (208K)
Unit 7.4 Metabolic Labeling with Fatty Acids
Abstract | Full Text: HTML PDF (175K)
Unit 7.5 Metabolic Labeling of Prenyl and Carboxyl-Methyl Groups
Abstract | Full Text: HTML PDF (144K)
Unit 11.7 Nuclear Import in Digitonin-Permeabilized Cells
Abstract | Full Text: HTML PDF (440K)
Unit 15.4 Determining Protein Transport to the Plasma Membrane
Abstract | Full Text: HTML PDF (150K)
Unit 16.3 Purification of Immunoglobulin G
Abstract | Full Text: HTML PDF (171K)
Unit 16.4 Fragmentation of Immunoglobulin G
Abstract | Full Text: HTML PDF (131K)
Appendix 1F Importing Biological Materials
Abstract | Full Text: HTML PDF (88K)
Supplement 4, October 1999
Unit 1.6 Media and Culture of Yeast
Abstract | Full Text: HTML PDF (177K)
Unit 3.3 Isolation of Mitochondria from Tissues and Cells by Differential Centrifugation
Abstract | Full Text: HTML PDF (188K)
Unit 3.4 Purification of a Crude Mitochondrial Fraction by Density-Gradient Centrifugation
Abstract | Full Text: HTML PDF (270K)
Unit 6.4 Two-Dimensional Gel Electrophoresis
Abstract | Full Text: HTML PDF (291K)
Unit 7.3 Metabolic Labeling with Sulfate
Abstract | Full Text: HTML PDF (99K)
Appendix 3A Molecular Biology Techniques
Abstract | Full Text: HTML PDF (38K)
Appendix 3B Spectrophotometric Determination of Protein Concentration
Appendix 3C Dialysis and Concentration of Protein Solutions
Abstract | Full Text: HTML PDF (166K)
Supplement 3, July 1999
Unit 5.4 Analysis of the Association of Proteins with Membranes
Abstract | Full Text: HTML PDF (230K)
Unit 14.4 Labeling Cultured Cells with 32 P i and Preparing Cell Lysates for Immunoprecipitation
Abstract | Full Text: HTML PDF (121K)
Unit 14.5 Phosphoamino Acid Analysis
Abstract | Full Text: HTML PDF (143K)
Unit 15.1 Overview of Protein Trafficking in the Secretory and Endocytic Pathways
Abstract | Full Text: HTML PDF (213K)
Unit 15.2 Use of Glycosidases to Study Protein Trafficking
Abstract | Full Text: HTML PDF (317K)
Unit 15.3 Endocytosis: Biochemical Analyses
Abstract | Full Text: HTML PDF (190K)
Unit 16.1 Production of Monoclonal Antibodies
Abstract | Full Text: HTML PDF (190K)
Unit 16.2 Production of Polyclonal Antisera
Abstract | Full Text: HTML PDF (133K)
Supplement 2, April 1999
Unit 3.2 Isolation of Rat Hepatocyte Plasma Membrane Sheets and Plasma Membrane Domains
Abstract | Full Text: HTML PDF (350K)
Unit 4.7 Cryo-Immunogold Electron Microscopy
Unit 8.5 Centrifugal Elutriation to Obtain Synchronous Populations of Cells
Abstract | Full Text: HTML PDF (246K)
Unit 10.5 Purification of Fibronectin
Abstract | Full Text: HTML PDF (132K)
Unit 10.6 Purification of Vitronectin
Abstract | Full Text: HTML PDF (91K)
Unit 11.5 In Vitro Analysis of SV 40 DNA Replication
Abstract | Full Text: HTML PDF (164K)
Unit 11.6 In Vitro Transcription
Abstract | Full Text: HTML PDF (211K)
Unit 12.4 Cell Wound Assays
Abstract | Full Text: HTML PDF (478K)
Supplement 1, January 1999
Unit 1.4 Sterilization and Filtration
Abstract | Full Text: HTML PDF (231K)
Unit 1.5 Assessing and Controlling Microbial Contamination in Cell Cultures
Abstract | Full Text: HTML PDF (463K)
Unit 2.4 Generation of Continuously Growing B Cell Lines by Epstein-Barr Virus Transformation
Abstract | Full Text: HTML PDF (101K)
Unit 4.5 Basic Confocal Microscopy
Abstract | Full Text: HTML PDF (358K)
Unit 4.6 Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues
Abstract | Full Text: HTML PDF (439K)
Unit 8.4 Determining Cell Cycle Stages by Flow Cytometry
Abstract | Full Text: HTML PDF (279K)
Unit 9.4 Analyzing Integrin-Dependent Adhesion
Unit 10.4 Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF - HR 9 Endodermal Cells
Abstract | Full Text: HTML PDF (283K)
Core Publication, October 1998
Unit 1.1 Basic Techniques for Mammalian Cell Tissue Culture
Full Text: HTML PDF (170K)
Unit 1.2 Media for Culture of Mammalian Cells
Abstract | Full Text: HTML PDF (225K)
Unit 1.3 Aseptic Technique for Cell Culture
Abstract | Full Text: HTML PDF (172K)
Unit 2.1 Establishment of Fibroblast Cultures
Abstract | Full Text: HTML PDF (707K)
Unit 2.2 Preparation and Culture of Human Lymphocytes
Abstract | Full Text: HTML PDF (166K)
Unit 2.3 Preparation of Endothelial Cells
Abstract | Full Text: HTML PDF (271K)
Unit 3.1 Overview of Cell Fractionation
Abstract | Full Text: HTML PDF (193K)
Unit 3.2 Isolation of Rat Hepatocyte Plasma Membrane Sheets and Plasma Membrane Domains
Unit 4.1 Proper Alignment and Adjustment of the Light Microscope
Abstract | Full Text: HTML PDF (929K)
Unit 4.2 Fluorescence Microscopy
Abstract | Full Text: HTML PDF (179K)
Unit 4.3 Immunofluorescence Staining
Abstract | Full Text: HTML PDF (229K)
Unit 4.4 Fluorescent Staining of Subcellular Organelles: ER, Golgi Complex, and Mitochondria
Abstract | Full Text: HTML PDF (503K)
Organelle Atlas: Appendix to Chapter 4
Abstract | Full Text: HTML PDF (2384K)
Unit 5.1 Overview of the Physical State of Proteins Within Cells
Abstract | Full Text: HTML PDF (187K)
Unit 5.2 Determining the Topology of an Integral Membrane Protein
Abstract | Full Text: HTML PDF (338K)
Unit 5.3 Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
Abstract | Full Text: HTML PDF (311K)
Unit 6.1 One-Dimensional SDS Gel Electrophoresis of Proteins
Full Text: HTML PDF (405K)
Unit 6.2 Immunoblotting and Immunodetection
Abstract | Full Text: HTML PDF (245K)
Unit 6.3 Detection and Quantitation of Radiolabeled Proteins in Gels and Blots
Abstract | Full Text: HTML PDF (134K)
Unit 7.1 Metabolic Labeling with Amino Acids
Abstract | Full Text: HTML PDF (147K)
Unit 7.2 Immunoprecipitation
Abstract | Full Text: HTML PDF (271K)
Unit 8.1 Overview of the Cell Cycle
Abstract | Full Text: HTML PDF (153K)
Unit 8.2 Assays for CDK Activity and DNA Replication in the Cell Cycle
Abstract | Full Text: HTML PDF (168K)
Unit 8.3 Methods for Synchronizing Cells at Specific Stages of the Cell Cycle
Abstract | Full Text: HTML PDF (213K)
Unit 9.1 Cell-Substrate Adhesion Assays
Abstract | Full Text: HTML PDF (140K)
Unit 9.2 Quantitative Measurement of Cell Adhesion Using Centrifugal Force
Abstract | Full Text: HTML PDF (183K)
Unit 9.3 Cadherin-Dependent Cell-Cell Adhesion
Abstract | Full Text: HTML PDF (162K)
Unit 10.1 Overview of Extracellular Matrix
Abstract | Full Text: HTML PDF (278K)
Unit 10.2 Preparation of Basement Membrane Components from EHS Tumors
Abstract | Full Text: HTML PDF (112K)
Unit 10.3 Preparation of Gelled Substrates
Abstract | Full Text: HTML PDF (143K)
Unit 11.1 Overview of Eukaryotic In Vitro Translation and Expression Systems
Abstract | Full Text: HTML PDF (149K)
Unit 11.2 In Vitro Translation
Abstract | Full Text: HTML PDF (206K)
Unit 11.3 In Vitro Analysis of Endoplasmic-Reticulum-to-Golgi Transport in Mammalian Cells
Abstract | Full Text: HTML PDF (276K)
Unit 11.4 Cotranslational Translocation of Proteins into Canine Rough Microsomes
Abstract | Full Text: HTML PDF (198K)
Unit 12.1 Chemotaxis Assays for Eukaryotic Cells
Abstract | Full Text: HTML PDF (871K)
Unit 12.2 Invasion Assays
Abstract | Full Text: HTML PDF (159K)
Unit 12.3 Cell Traction
Abstract | Full Text: HTML PDF (441K)
Unit 13.1 Microtubule/Organelle Motility Assays
Abstract | Full Text: HTML PDF (263K)
Unit 13.2 In Vitro Motility Assay to Study Translocation of Actin by Myosin
Abstract | Full Text: HTML PDF (118K)
Unit 14.1 Overview of Protein Phosphorylation
Abstract | Full Text: HTML PDF (84K)
Unit 14.2 Immunological Detection of Phosphorylation
Abstract | Full Text: HTML PDF (234K)
Unit 14.3 The Detection of MAPK Signaling
Appendix 1A Useful Measurements and Data
Abstract | Full Text: HTML PDF (46K)
Appendix 1B Compendium of Drugs Commonly Used in Cell Biology Research
Abstract | Full Text: HTML PDF (370K)
Appendix 1C Identification of Motifs in Protein Sequences
Abstract | Full Text: HTML PDF (205K)
Appendix 1D Safe Use of Radioisotopes
Abstract | Full Text: HTML PDF (179K)
Appendix 1E Absorption and Emission Maxima for Common Fluorophores
Abstract | Full Text: HTML PDF (101K)
Appendix 1F Importing Biological Materials
Appendix 1G Centrifuges and Rotors
Appendix 1H Internet Basics for Biologists
Abstract | Full Text: HTML PDF (164K)
Appendix 2A Common Stock Solutions, Buffers, and Media
Abstract | Full Text: HTML PDF (181K)
Appendix 2B Medium Formulations
Abstract | Full Text: HTML PDF (114K)
Appendix 2C Standard Laboratory Equipment
Abstract | Full Text: HTML PDF (89K)
Selected Suppliers of Reagents and Equipment
Abstract | Full Text: HTML PDF (180K)