Current Protocols in Cell Biology
Online ISBN: 9780471143031
Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment's success are not captured in the primary literature but exist only as part of a lab's oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Quarterly installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.
Current Protocols in Cell Biology is the comprehensive source for protocols for the multidisciplinary field of cell biology, providing an extensive range of protocols for the analysis of cell structures and functions. Authored by scientists from around the world, these protocols permit investigators to analyze the cell at the molecular level, as a single cell, as well as a tissue in experiments exploring cellular and organelle motility, cell adhesion, protein trafficking, the cell cycle, aging and death, signal transduction pathways, and chromosomes and nuclei. Techniques include basic and cutting edge methods for isolation of primary cells, isolation of organelles, microscopic analysis of cell and molecular behavior including the use of fluorescent proteins, protein characterization, labeling molecules, cell cycle analysis, isolation and use of extracellular matrix molecules, in vitro reconstitution of cellular and intracellular events, detection of macromolecular interactions, and differentiation of embryonic and adult stem cells. With quarterly updates, Current Protocols in Cell Biology continues to add new and updated protocols to the content.
The target audience for Current Protocols in Cell Biology is anyone who is conducting research in the broad subject of cell biology, with only a minimum of basic laboratory experience. Sufficient detail is provided to permit duplication of the protocols in any laboratory, whatever the disciplinary background or level of sophistication.
cell biologists • immunologists • molecular biologists • developmental biologists • biologists • microscopists • cytologists • cell culturists • cellular physiologists
cell biology, microscopy, immunotechniques, cell culture, motility, signal transduction, trafficking, chromosomes, extracellular matrix, cell adhesion, cell physiology, cytology, histochemistry, fractionation, organelle, cell cycle, apoptosis, cell death, secretion, endocytosis, protein synthesis, lipid transport, cell migration, model organisms, laboratory protocols, Current Protocols