Culture of Cells for Tissue Engineering

Culture of Cells for Tissue Engineering

Editor(s): Gordana Vunjak-Novakovic, R. Ian Freshney

Published Online: 9 FEB 2006

Print ISBN: 9780471629351

Online ISBN: 9780471741817

DOI: 10.1002/0471741817

Series Editor(s): R. Ian Freshney

About this Book

Step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering

Tissue engineering is a multidisciplinary field incorporating the principles of biology, chemistry, engineering, and medicine to create biological substitutes of native tissues for scientific research or clinical use. Specific applications of this technology include studies of tissue development and function, investigating drug response, and tissue repair and replacement. This area is rapidly becoming one of the most promising treatment options for patients suffering from tissue failure.

Written by leading experts in the field, Culture of Cells for Tissue Engineering offers step-by-step, practical guidance for the acquisition, manipulation, and use of cell sources for tissue engineering. It offers a unique focus on tissue engineering methods for cell sourcing and utilization, combining theoretical overviews and detailed procedures.

Features of the text include:

  • Easy-to-use format with a two-part organization
  • Logically organized-part one discusses cell sourcing, preparation, and characterization and the second part examines specific engineered tissues
  • Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
  • End-of-chapter features include useful listings of sources for reagents, materials, and supplies, with the contact details of the suppliers listed at the end of the book
  • A section of elegant color plates to back up the figures in the chapters

Culture of Cells for Tissue Engineering gives novice and seasoned researchers in tissue engineering an invaluable resource. In addition, the text is suitable for professionals in related research, particularly in those areas where cell and tissue culture is a new or emerging tool.

Table of contents

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    3. Chapter 3

      Human Embryonic Stem Cell Culture for Tissue Engineering (pages 61–82)

      Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller and Robert Langer

    4. Chapter 8

      Ligament Tissue Engineering (pages 191–211)

      Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan and Gregory H. Altman

    5. Chapter 9

      Cellular Photoencapsulation in Hydrogels (pages 213–238)

      Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim and Christopher Williams

    6. Chapter 10

      Tissue Engineering Human Skeletal Muscle for Clinical Applications (pages 239–257)

      Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey and Herman H. Vandenburgh

    7. Chapter 11

      Engineered Heart Tissue (pages 259–291)

      Thomas Eschenhagen and Wolfgang H. Zimmermann

    8. Chapter 13

      Tissue Engineering of Bone (pages 323–373)

      Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic and Lorenz Meinel

    9. Chapter 14

      Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering (pages 375–415)

      Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron and Gianluca Gallo

    10. Chapter 15

      Tissue Engineering of the Liver (pages 417–471)

      Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani and Sangeeta N. Bhatia

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