Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis

Editor(s): Susanne Brakmann, Kai Johnsson

Published Online: 28 MAR 2003

Print ISBN: 9783527304233

Online ISBN: 9783527600649

DOI: 10.1002/3527600647

About this Book

Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity.
The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!

Table of contents

    1. You have free access to this content
    2. Chapter 1

      Introduction (pages 1–3)

      Prof. Dr. Kai Johnsson and Dr. Susanne Brakmann

    3. Chapter 7

      Yeast n-Hybrid Systems for Molecular Evolution (pages 127–158)

      Brian T. Carter, Hening Lin and Prof. Dr. Virginia W. Cornish

    4. Chapter 9

      Engineering Protein Evolution (pages 177–213)

      Dr. Stefan Lutz and Prof. Dr. Stephen J. Benkovic

    5. Chapter 12

      Applied Molecular Evolution of Enzymes Involved in Synthesis and Repair of DNA (pages 281–307)

      John F. Davidson, Jon Anderson, Haiwei Guo, Daniel Landis and Prof. Dr. Lawrence A. Loeb