Directed Molecular Evolution of Proteins: or How to Improve Enzymes for Biocatalysis
Copyright © 2002 Wiley-VCH Verlag GmbH & Co. KGaA
Editor(s): Susanne Brakmann, Kai Johnsson
Published Online: 28 MAR 2003
Print ISBN: 9783527304233
Online ISBN: 9783527600649
About this Book
Natural selection created optimal catalysts. However, optimal performance of enzyme catalysis does not necessarily refer to maximum reaction rate. Rather, it may be a compromise between specificity, rate, stability, and other chemical constraints that makes enzymes capable of catalyzing reactions under mild conditions and with high substrate specificity, accompanied by high regio- and enantioselectivity.
The book presented here focuses on the directed evolution of proteins, which has established itself as a powerful method for designing enzymes showing new substrate specificities. It includes a comprehensive repertoire of techniques for producing combinatorial enzyme libraries, while the functional gene expression in a suitable host helps in selecting the appropriate structure, making fast screening a necessity. This book illustrates both the theoretical background as well as the potential of this interesting method in practice - which is becoming ever more important even in classical organic synthesis!