Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls

Analysing Gene Expression: A Handbook of Methods: Possibilities and Pitfalls

Editor(s): Stefan Lorkowski, Paul Cullen

Published Online: 27 MAY 2004

Print ISBN: 9783527304882

Online ISBN: 9783527601493

DOI: 10.1002/352760149X

About this Book

This book combines the experience of 225 experts on 900 pages.

Scientists worldwide are currently overwhelmed by the ever-increasing number and diversity of genome projects. This handbook is your guide through the jungle of new methods and techniques available to analyse gene expression - the first to provide such a broad view of the measurement of mRNA and protein expression in vitro, in situ and even in vivo. Despite this broad approach, detail is sufficient for you to grasp the principles behind each method. In each case, the authors weigh up the advantages and disadvantages, paying particular attention to the automated, high-throughput processing demanded by the biotech industry. Completely up to date, the book covers such ground-breaking methods such as DNA microarrays, serial analysis of gene expression, differential display, and identification of open reading frame expressed sequence tags. All the methods and necessary equipment are presented visually in more than 300 mainly colour illustrations to assist their step-by-step reproduction in your laboratory. Each chapter is rounded off with its own set of extensive references that provide access to detailed experimental protocols. In short, the bible of analysing gene expression.

Table of contents

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  1. Part 1: Volume 1

    1. Chapter 1

      Basic Concepts of Gene Expression (pages 1–95)

      Dr. Paul Cullen, Dr. Stefan Lorkowski, Mario Kratz, Michel Werner, Christoph Marschall, Hanns-Georg Klein, David Goodlett, Ruedi Aebersold and Claudia Wenner

    2. Chapter 2

      Sample Preparation and Supplementary Tools (pages 97–161)

      Dr. Stefan Lorkowski, Dr. Paul Cullen, Giovanni Salerno, Renate Burgemeister, Karin Schütze, Douglas J. McGarvey, Achim Quandt and Jörg Haberland

    3. Chapter 3

      Methods for Analysing mRNA Expression (pages 163–407)

      Dr. Stefan Lorkowski, Dr. Paul Cullen, Sebastian Horstmann, Beate Hess, Claus Langer, Udo Seedorf, Rahul V. Gopalkrishnan, Devanand Sarkar, Dong-Chul Kang, Frank Sivo, Irina V. Lebedeva, Magdalena Leszczyniecka, Moira Sauane, Neil I. Goldstein, Paul B. Fisher, Zao-Zhong Su, Carsten Gerdemann, Anne Sherwood, Gobinda Sarkar, Kunbo Zhang, Mark E. Bolander, Jian-Hua Luo, Yan Ping Yu, Katrin Stolle, Zhou Wang, Donald D. Brown, Yuxun Wang, Arthur J. Sytkowski, David J. Volsky, Hongping Jiang, Lucas D. Bowler, Annabelle Bouchardon, Colette Dehay, Daniel J. Lavery, Karl J. Lackner, Thomas Roeder, Iris Bruchhaus, Benoît Kornmann, Daniele Rifat, Nicolas Preitner, Ueli Schibler, Stephen A. Bustin, Kim Theilgaard-Mönch, Thomas Rasmussen, Michael Schnoor, Kikuya Kato, Christian W. B. Bachem, Asun Fernandes del Carmen, Richard G. F. Visser, Cruz A. Hinojos, Peter A. Doris, Sergey V. Shmelkov, Alexander V. Belyavsky, Michael P. Starkey, Harin Mahadeva, Sabine Bahn, Chihiro Uematsu, Fumihito Miura, Hideki Kambara, Junko Nishida, Kazunori Okano, Takashi Ito, Yoshiyuki Sakaki, Mikhail V. Matz and Sachihiko Watanabe

  2. Part 2: Volume 2

    1. Chapter 4

      High-Throughput and Industrial Methods for mRNA Expression Analysis (pages 409–622)

      Dr. Paul Cullen, Dr. Stefan Lorkowski, Oliver Bauer, Anna Guerasimova, Hans Lehrach, Michal Janitz, Ralf Herwig, Uwe Radelof, Wanda Panter, Jennifer Harrington, Gerhard Lorkowski, Alon Amit, Ralf Bickel, Annette Wagenhaus, Eugen Ermantraut, Gerd Wagner, Ines Leube, Jana Sachtschal, Ralf Ehricht, Thomas Ellinger, Zhijian J. Chen, Kenneth D. Tew, Laurent Gate, Warren Davis, Hermann Lübbert, Jeffrey S. Fisher, Jamie Walden, Jonathan Meade, Peng Liang, Zhen Guo, Richard A. Shimkets, Kousaku Okubo, Shoko Kawamoto, Jill M. Ray, Michael C. MacLeod, Brian S. Hilbush, David Lo, J. Gregor Sutcliffe, Lianggui Chen, Rick Woychik, Glenn Albrecht, Kevin Corcoran, Norrie Russell, Sydney Brenner, Tim Burcham, Vivian de Waard, Anton-Jan van Zonneveld, Hans Pannekoek, Nicholas J. Beauchamp, Nicole A. Datson, Erno Vreugdenhil, Jo-Ann L. Stanton, David P. L. Green, Jean-Marc Elalouf and Dominic G. Spinella

    2. Chapter 5

      Protein Expression Analysis (pages 623–702)

      Simone König, Jörg Haberland, Jens Grote, Martin Zeller, Erk Gedig, Manfred Fobker, Michael Erren, Stephen W. Pursley, John E. Pearl, Dr. Paul Cullen and Dr. Stefan Lorkowski

    3. Chapter 6

      Methods for mRNA and Protein Expression Analysis in situ and in vivo (pages 703–767)

      Dr. Paul Cullen, Dr. Stefan Lorkowski, Clive N. Kind, Michael Schnoor, Christoph Bremer, Ralph Weissleder, Heiko Alfke, Johannes T. Heverhagen, Thomas M. Behr and Martin P. Béhé

    4. Chapter 7

      Computational Methods and Bioinformatic Tools (pages 769–904)

      Dr. Paul Cullen, Dr. Stefan Lorkowski, Steffen Hennig, Albert Poustka, Georgia Panopoulou, Hans Lehrach, Eberhard Korsching, Oxana Pickeral, Ralf Herwig, Alexander Kel, Dmitrij Tchekmenev, Edgar Wingender, Johannes Streicher, Gerd B. Müller, Takeshi Kawashima, Kazuhiro W. Makabe, Inna Dubchak, Hongkai Ji, Kousaku Okubo, Shoko Kawamoto, Ellen Fricke, Dagmar Karas, Martin Haubrock, Sigrid Land, Stella Rotert, Xin Chen, Joan Pontius, Eric Eveno, Charles Auffray, Charles Decraene, Claude Chelala, Geneviève Piétu, Marie-Dominique Devignes, Régine Mariage-Samson, Sandrine Imbeaud, Sylvie Bortoli, Alon Amit, Martin Ringwald, Michael J. de Veer, Bryan R. G. Williams, Eldon M. Walker, Jamie A. Davies, Christoph Grunau, Richard Baldock, Duncan R. Davidson, Christian J. Stoeckert, Angel Pizarro, Elisabetta Manduchi, Gregory R. Grant, Jonathan Crabtree, Junmin Liu, Phuc V. Le, Shannon K. McWeeney, Stephen Welle, Catherine A. Ball, David Botstein, Gail Binkley, Gavin J. Sherlock, J. Michael Cherry, Kara Dolinski, Laurie Issel-Tarver, Mark Schroeder, Selina S. Dwight, Shuai Wenig, John C. Matese, Heng Jin, Jeremy Gollub, Joan Hebert, Miroslava Kaloper, Patrick O. Brown, Tina Hernandez-Boussard, Anuj Kumar, Kei-Hoi Cheung, Luis Marenco, Michael Snyder, Nick Tosches, Paul Bertone, Perry Miller, Peter Masiar, Yang Liu, Graziano Pesole, Philippe Marc, Margaret Biswas, Paul Kersey, Rolf Apweiler and Christine Hoogland

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