Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Production of Recombinant Proteins: Novel Microbial and Eukaryotic Expression Systems

Editor(s): Gerd Gellissen

Published Online: 19 MAY 2005

Print ISBN: 9783527310364

Online ISBN: 9783527603671

DOI: 10.1002/3527603670

About this Book

While the choices of microbial and eukaryotic expression systems for production of recombinant proteins are many, most researchers in academic and industrial settings do not have ready access to pertinent biological and technical information since it is normally scattered throughout the scientific literature. This book closes the gap by providing information on the general biology of the host organism, a description of the expression platform, a methodological section -- with strains, genetic elements, vectors and special methods, where applicable -- as well as examples of proteins produced with the respective platform. The systems thus described are well balanced by the inclusion of three prokaryotes (two Gram-negatives and one Gram-positive), four yeasts, two filamentous fungi and two higher eukaryotic cell systems -- mammalian and plant cells. Throughout, the book provides valuable practical and theoretical information on the criteria and schemes for selecting the appropriate expression platform, the possibility and practicality of a universal expression vector, and on comparative industrial-scale fermentation, with the production of a recombinant Hepatitis B vaccine chosen as an industrial example.

With a foreword by Herbert P. Schweizer, Colorado State University, USA:
"As a whole, this book is a valuable and overdue resource for a varied audience. It is a practical guide for academic and industrial researchers who are confronted with the design of the most suitable expression platform for their favorite protein for technical or pharmaceutical purposes. In addition, the book is also a valuable study resource for professors and students in the fields of applied biology and biotechnology."

Table of contents

    1. You have free access to this content
    2. Chapter 1

      Key and Criteria to the Selection of An Expression Platform (pages 1–5)

      Prof. Dr. Gerd Gellissen, Alexander W.M. Strasser and Manfred Suckow

    3. Chapter 3

      Pseudomonas fluorescens (pages 45–66)

      Lawrence C. Chew, Tom M. Ramseier, Diane M. Retallack, Jane C. Schneider, Charles H. Squires and Henry W. Talbot

    4. Chapter 5

      Arxula adeninivorans (pages 89–110)

      Erik Böer, Prof. Dr. Gerd Gellissen and Gotthard Kunze

    5. Chapter 6

      Hansenula polymorpha (pages 111–142)

      Hyun Ah Kang and Prof. Dr. Gerd Gellissen

    6. Chapter 7

      Pichia pastoris (pages 143–162)

      Christine Ilgen, Joan Lin-Cereghino and James M. Cregg

    7. Chapter 8

      Yarrowia lipolytica (pages 163–189)

      Catherine Madzak, Jean-Marc Nicaud and Claude Gaillardin

    8. Chapter 9

      Aspergillus sojae (pages 191–214)

      Margreet Heerikhuisen, Cees van den Hondel and Peter Punt

    9. Chapter 11

      Mammalian Cells (pages 233–252)

      Volker Sandig, Thomas Rose, Karsten Winkler and Rene Brecht

    10. Chapter 12

      Plant Cells (pages 253–272)

      Rainer Fischer, Richard M Twyman, Jürgen Drossard, Stephan Hellwig and Stefan Schillberg

    11. Chapter 13

      Wide-Range Integrative Expression Vectors for Fungi, Based on Ribosomal DNA Elements (pages 273–286)

      Jens Klabunde, Gotthard Kunze, Prof. Dr. Gerd Gellissen and Cornelis P. Hollenberg

    12. Chapter 14

      Comparative Fermentation (pages 287–317)

      Stephan Hellwig, Christoph Stöckmann, Prof. Dr. Gerd Gellissen and Jochen Büchs

    13. Chapter 15

      Recombinant Hepatitis B Vaccines: Disease Characterization and Vaccine Production (pages 319–359)

      Pascale Brocke, Stephan Schaefer, Karl Melber, Volker Jenzelewski, Frank Müller, Ulrike Dahlems, Oliver Bartelsen, Kyung-Nam Park, Zbigniew A. Janowicz and Prof. Dr. Gerd Gellissen

    14. You have free access to this content