Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution

Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution

Editor(s): Susanne Brakmann, Andreas Schwienhorst

Published Online: 13 MAY 2005

Print ISBN: 9783527307999

Online ISBN: 9783527603695

DOI: 10.1002/3527603697

About this Book

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed.
From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.
Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.
As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.
In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

Table of contents

    1. You have free access to this content
    2. Chapter 1

      Introduction (pages 1–3)

      Dr. Susanne Brakmann and Dr. Andreas Schwienhorst

    3. Chapter 3

      DNA Shuffling (pages 13–24)

      Hikaru Suenaga, Masatoshi Goto and Kensuke Furukawa

    4. Chapter 7

      Selection of Aptamers (pages 65–86)

      Heiko Fickert, Heike Betat and Ulrich Hahn

    5. Chapter 11

      Directed in silico Mutagenesis (pages 153–175)

      Markus Wiederstein, Peter Lackner, Ferry Kienberger and Manfred J. Sippl

    6. Chapter 12

      RNA Folding in silico (pages 177–190)

      Christoph Flamm, Ivo L. Hofacker and Peter F. Stadler

    7. You have free access to this content