Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

Online ISBN: 9780470089941

DOI: 10.1002/9780470089941

Mission Statement

Sound and reproducible laboratory methods are the foundation of scientific discovery. Yet, all too often, nuances that are critical for an experiment’s success are not captured in the primary literature but exist only as part of a lab’s oral tradition. The aim of each Current Protocols title is to provide the clearest, most detailed and reliable step-by-step instructions for all protocols relevant to a given subject area. Written by experts in the field and extensively edited to our exacting standards, each article also provides invaluable discussions of background information, applications of the methods, important assumptions, key parameters, troubleshooting tips, time considerations, and more. Furthermore, Current Protocols content is thoughtfully organized by topic for optimal usage and to maximize contextual knowledge. Regular installments allow Current Protocols to constantly evolve to keep pace with the newest discoveries and developments.

Current Protocols Essential Laboratory Techniques seeks to bridge the gap between the theory of the classroom and its application in the laboratory. By codifying and verifying basic information often taken for granted by more senior members of the lab, and presenting it in a clear and direct format, this title is intended to provide a resource upon which novice researchers can rely to become productive members of the modern life science laboratory. Current Protocols Essential Laboratory Techniques also serves to combat the so-called “kit mentality” by explaining the rationale behind many of the kits in common use today. The ultimate goal of Current Protocols Essential Laboratory Techniques is to equip the reader with a solid skill set that will continue to be applied and improved over the course of his or her research career.

The target audience of this title is the novice researcher entering or preparing to enter the modern life science laboratory for the very first time. It is assumed that readers will have taken introductory courses in chemistry, biology, and physics. Although some may also have more advanced training, it is assumed that the reader has no practical experience outside of introductory laboratory courses. Therefore, these articles will serve as his or her first experience with the techniques described.


Biologists • life scientists • molecular biologists • immunologists • cell biologists • biochemists • microbiologists • neuroscientists • pharmaceutical scientists


life science, biology, molecular biology, instrumentation, reagent preparation, cell culture, chromatography, electrophoresis, microscopy, data analysis, data presentation, laboratory protocols, Current Protocols.