Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques

Online ISBN: 9780470089941

DOI: 10.1002/9780470089941

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  1. Foreword
  2. Preface
  3. Common Conversion Factors
  4. UNIT FM02 Combining Techniques to Answer Molecular Questions
  5. Chapter 1 Volume/Weight Measurement
    1. UNIT 1.1 Volume Measurement
    2. UNIT 1.2 Weight Measurement
  6. Chapter 2 Concentration Measurement
    1. UNIT 2.1 Spectrophotometry
    2. UNIT 2.2 Quantitation of Nucleic Acids and Proteins
    3. UNIT 2.3 Radiation Safety and Measurement
    4. UNIT 2.4 Fluorescence Spectroscopy
  7. Chapter 3 Reagent Preparation
    1. UNIT 3.1 Reagent Preparation: Theoretical and Practical Discussions
    2. UNIT 3.2 Measurement of pH
    3. UNIT 3.3 Recipes for Commonly Encountered Reagents
  8. Chapter 4 Cell Culture Techniques
    1. UNIT 4.1 Aseptic Technique
    2. UNIT 4.2 Culture of Escherichia coli and Related Bacteria
    3. UNIT 4.3 Mammalian Cell Culture
    4. UNIT 4.4 Isolation, Culture, and Characterization of Bacteriophages
  9. Chapter 5 Sample Preparation
    1. UNIT 5.1 Centrifugation
    2. UNIT 5.2 Purification and Concentration of Nucleic Acids
  10. Chapter 6 Chromatography
    1. UNIT 6.1 Principles of Chromatography
    2. UNIT 6.2 Column Chromatography
    3. UNIT 6.3 Thin Layer Chromatography
  11. Chapter 7 Electrophoresis
    1. UNIT 7.1 Overview of Electrophoresis
    2. UNIT 7.2 Agarose Gel Electrophoresis
    3. UNIT 7.3 SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
    4. UNIT 7.4 Staining Proteins in Gels
    5. UNIT 7.5 Overview of Digital Electrophoresis Analysis
  12. Chapter 8 Blotting
    1. UNIT 8.1 Overview of Blotting
    2. UNIT 8.2 Nucleic Acid Blotting: Southern and Northern
    3. UNIT 8.3 Protein Blotting: Immunoblotting
    4. UNIT 8.4 Labeling DNA and Preparing Probes
  13. Chapter 9 Microscopy and Imaging
    1. UNIT 9.1 Conventional Light Microscopy
    2. UNIT 9.2 Immunofluorescence Microscopy
    3. UNIT 9.3 In Situ Hybridization: Fruit Fly Embryos and Tissues
    4. UNIT 9.4 Confocal Microscopy
    5. UNIT 9.5 Objective Morphological Quantification of Microscopic Images Using a Fast Fourier Transform (FFT) Analysis
  14. Chapter 10 Enzymatic Reactions
    1. UNIT 10.1 Working with Enzymes
    2. UNIT 10.2 Overview of PCR
    3. UNIT 10.3 Real-Time PCR
    4. UNIT 10.4 DNA Sequencing: An Outsourcing Guide
    5. UNIT 10.5 Overview of qPCR Molecular Probes
  15. Chapter 11 Bioinformatics and Genomics Tools
    1. UNIT 11.1 Using NCBI BLAST
    2. UNIT 11.2 Submitting a Sequence to GenBank
    3. UNIT 11.3 Beyond Simple Homology Searches: Multiple Sequence Alignments and Phylogenetic Trees
    4. UNIT 11.4 Using Model Organism Databases (MODs)
    5. UNIT 11.5 Whole-Genome Sequencing Analysis Using Next-Generation Sequencing Data
  16. Chapter 12 Outsourcing Guides
    1. UNIT 12.1 DNA Sequencing: An Outsourcing Guide
    2. UNIT 12.2 Mass Spectrometry: An Outsourcing Guide
  17. Chapter 13 Manipulating DNA and Gene Expression
    1. UNIT 13.1 LOV Domains as In Vivo Fluorescent Reporters of Protein Expression
    2. UNIT 13.2 Expression of Recombinant Genes in the Yeast Pichia pastoris
    3. UNIT 13.3 Isolation of Bacteriophages from Environmental Sources, and Creation and Functional Screening of Phage DNA Libraries
  18. Appendix 1 Safety
    1. APPENDIX 1A General Laboratory Safety and Working with Hazardous Chemicals
    2. APPENDIX 1B Risk Assessment in the Research Laboratory
  19. Appendix 2 Experiment Documentation and Data Storage
    1. APPENDIX 2A Laboratory Notebooks and Data Storage
  20. Appendix 3 Considerations When Altering Digital Images
    1. APPENDIX 3A Ethical Considerations When Altering Digital Images
    2. APPENDIX 3B Practical Considerations When Altering Digital Images
    3. APPENDIX 3C Digital Image Processing and Analysis with ImageJ
  21. Appendix 4 Data Analysis
    1. APPENDIX 4A Statistical Analysis
    2. APPENDIX 4B Basics of Statistical Analysis
  22. Appendix 5 Getting Your Data Out Into the World
    1. APPENDIX 5A Preparing and Presenting a Poster
    2. APPENDIX 5B Preparing and Presenting a Talk
    3. APPENDIX 5C How to Write a Research Manuscript
    4. APPENDIX 5D Bibliographies Made Easy: A Guide to Using Citation Software to Create Bibliographies

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