Current Protocols in Stem Cell Biology
Online ISBN: 9780470151808
DOI: 10.1002/9780470151808
Archive
Current Protocols in Stem Cell Biology Supplements
2017: Supplement 41 Supplement 40
2016: Supplement 39 Supplement 38 Supplement 37 Supplement 36
2015: Supplement 35 Supplement 34 Supplement 33 Supplement 32
2014: Supplement 31 Supplement 30 Supplement 29 Supplement 28
2013: Supplement 27 Supplement 26 Supplement 25 Supplement 24
2012: Supplement 23 Supplement 22 Supplement 21 Supplement 20
2011: Supplement 19 Supplement 18 Supplement 17 Supplement 16
2010: Supplement 15 Supplement 14 Supplement 13 Supplement 12
2009: Supplement 11 Supplement 10 Supplement 9 Supplement 8
2008: Supplement 7 Supplement 6 Supplement 5 Supplement 4
2007: Supplement 3 Supplement 2 Supplement 1
Supplement 41, May 2017
Unit 1C.20 Isolation of Stem Cells and Progenitors from Mouse Epidermis
Abstract | Full Text: HTML PDF
Unit 1F.17 Isolation of Ready-to-Use Adipose-Derived Stem Cell (ASC) Pellet for Clinical Applications and a Comparative Overview of Alternate Methods for ASC Isolation
Abstract | Full Text: HTML PDF
Unit 1F.18 Human Umbilical Cord Mesenchymal Stromal Cell Isolation, Expansion, Cryopreservation, and Characterization
Abstract | Full Text: HTML PDF
Unit 1F.19 Angiogenesis within Stem Cell–Seeded Silk Scaffolds Cultured on the Chorioallantoic Membrane and Visualized by 3D Imaging
Abstract | Full Text: HTML PDF
Unit 4A.11 Rhesus Macaque iPSC Generation and Maintenance
Abstract | Full Text: HTML PDF
Unit 5A.9 Tetracycline-Inducible and Reversible Stable Gene Expression in Human iPSC-Derived Neural Progenitors and in the Postnatal Mouse Brain
Abstract | Full Text: HTML PDF
Supplement 40, February 2017
Unit 1D.9 Purification of Definitive Endoderm Generated from Pluripotent Stem Cells by Magnetic Cell Sorting
Abstract | Full Text: HTML PDF
Unit 1F.16 Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling
Abstract | Full Text: HTML PDF
Unit 2D.19 Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes
Abstract | Full Text: HTML PDF
Unit 4A.10 Reprogramming of Pancreatic Acinar Cells to Functional Beta Cells by In Vivo Transduction of a Polycistronic Construct Containing Pdx1, Ngn3, MafA in Mice
Abstract | Full Text: HTML PDF
Supplement 39, November 2016
Unit 1F.15 Fast and Efficient Neural Conversion of Human Hematopoietic Cells
Abstract | Full Text: HTML PDF
Unit 1H.10 Derivation of Telencephalic Oligodendrocyte Progenitors from Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 2D.18 Efficient Generation of Viral and Integration-Free Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes
Abstract | Full Text: HTML PDF
Unit 4A.6 cGMP Generation of Human Induced Pluripotent Stem Cells with Messenger RNA
Abstract | Full Text: HTML PDF
Supplement 38, August 2016
Unit 1G.6 Small-Molecule-Directed Hepatocyte-Like Cell Differentiation of Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 2A.4 Isolation and Assessment of Single Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
Abstract | Full Text: HTML PDF
Unit 2D.7 Neural Stem Cell or Human Induced Pluripotent Stem Cell–Derived GABA-ergic Progenitor Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy
Abstract | Full Text: HTML PDF
Unit 2D.18 Efficient Generation of Viral and Integration-Free Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes
Abstract | Full Text: HTML PDF
Unit 4A.9 Derivation of Transgene-Free Induced Pluripotent Stem Cells from a Single Drop of Blood
Abstract | Full Text: HTML PDF
Unit 5B.5 Self-Cloning CRISPR
Abstract | Full Text: HTML PDF
Unit 5B.6 Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 37, May 2016
Unit 1C.19 A Method for Sectioning and Immunohistochemical Analysis of Stem Cell–Derived 3-D Organoids
Abstract | Full Text: HTML PDF
Unit 1E.9 Production of 3-D Airway Organoids From Primary Human Airway Basal Cells and Their Use in High-Throughput Screening
Abstract | Full Text: HTML PDF
Unit 1E.10 Isolation of Human Amnion Epithelial Cells According to Current Good Manufacturing Procedures
Abstract | Full Text: HTML PDF
Supplement 36, February 2016
Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1C.15 Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds
Abstract | Full Text: HTML PDF
Unit 1G.3 Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors
Abstract | Full Text: HTML PDF
Unit 5A.2 Identifying Adult Stem Cells Using Cre-Mediated Lineage Tracing
Abstract | Full Text: HTML PDF
Unit 5B.4 Delivery of Genome Editing Reagents to Hematopoietic Stem/Progenitor Cells
Abstract | Full Text: HTML PDF
Supplement 35, November 2015
Unit 1C.14 Standardized Cryopreservation of Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
Abstract | Full Text: HTML PDF
Unit 1E.7 Quantifying Epithelial Early Common Progenitors from Long-Term Primary or Cell Line Sphere Culture
Abstract | Full Text: HTML PDF
Unit 2C.5 The Derivation of Primary Human Epicardium-Derived Cells
Abstract | Full Text: HTML PDF
Unit 5A.8 CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 34, August 2015
Unit 2E.3 Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries
Abstract | Full Text: HTML PDF
Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
Abstract | Full Text: HTML PDF
Unit 2F.2 Regional Cell Specific RNA Expression Profiling of FACS Isolated Drosophila Intestinal Cell Populations
Abstract | Full Text: HTML PDF
Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
Abstract | Full Text: HTML PDF
Unit 5B.3 TALEN- and CRISPR/Cas9-Mediated Gene Editing in Human Pluripotent Stem Cells Using Lipid-Based Transfection
Abstract | Full Text: HTML PDF
Supplement 33, May 2015
Unit 1F.14 Isolation and Expansion of Mesenchymal Stem Cells from Human Conjunctival Tissue
Abstract | Full Text: HTML PDF
Unit 1H.9 Neural Crest Cells from Dual SMAD Inhibition
Abstract | Full Text: HTML PDF
Supplement 32, February 2015
Unit 1C.18 Efficient and Scalable Culture of Single Dissociated Human Pluripotent Stem Cells Using Recombinant E8 Fragments of Human Laminin Isoforms
Abstract | Full Text: HTML PDF
Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
Abstract | Full Text: HTML PDF
Unit 1H.8 Generation of Highly Enriched Populations of Optic Vesicle–Like Retinal Cells from Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 4A.8 Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research
Abstract | Full Text: HTML PDF
Supplement 31, November 2014
Unit 1A.6 Generation of Mammalian Offspring by Haploid Embryonic Stem Cells Microinjection
Abstract | Full Text: HTML PDF
Unit 1C.17 Cryopreservation of Human Pluripotent Stem Cells in Defined Medium
Abstract | Full Text: HTML PDF
Unit 2D.17 Isolation and Long-Term Expansion of Functional, Myelinating Oligodendrocyte Progenitor Cells from Neonatal Rat Brain
Abstract | Full Text: HTML PDF
Unit 4A.7 Laser-Assisted Generation of Human Induced Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 30, August 2014
Unit 1G.5 Deriving Functional Hepatocytes from Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 29, May 2014
Unit 1C.16 Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns
Abstract | Full Text: HTML PDF
Unit 1H.7 Slice Culture Method for Studying Migration of Neuronal Progenitor Cells Derived from Human Embryonic Stem Cells (hESC)
Abstract | Full Text: HTML PDF
Unit 5A.7 Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease
Abstract | Full Text: HTML PDF
Supplement 28, February 2014
Unit 1C.14 Standardized Cryopreservation of Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 1C.15 Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds
Abstract | Full Text: HTML PDF
Unit 2B.6 Preparation of Anti-Inflammatory Mesenchymal Stem/Precursor Cells (MSCs) Through Sphere Formation Using Hanging-Drop Culture Technique
Abstract | Full Text: HTML PDF
Supplement 27, November 2013
Unit 2B.5 Preparation and Osteogenic Differentiation of Scaffold-Free Mouse Adipose-Derived Stromal Cell Microtissue Spheroids (ASC-MT)
Abstract | Full Text: HTML PDF
Unit 2F.2 RNA Expression Profiling from FACS-Isolated Cells of the Drosophila Intestine
Abstract | Full Text: HTML PDF
Unit 4A.6 Feeder-Free Reprogramming of Human Fibroblasts with Messenger RNA
Abstract | Full Text: HTML PDF
Unit 5A.6 Retroviral Gene Expression Control in Primary Organoid Cultures
Abstract | Full Text: HTML PDF
Supplement 26, August 2013
Unit 1G.4 Differentiation of Hepatocytes from Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 2B.4 Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Abstract | Full Text: HTML PDF
Unit 2D.16 Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
Abstract | Full Text: HTML PDF
Unit 3.8 Isolation of Human Melanoma Stem Cells Using ALDH as a Marker
Abstract | Full Text: HTML PDF
Unit 5C.3 Array-Based High-Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
Abstract | Full Text: HTML PDF
Supplement 25, May 2013
Unit 1B.10 The Streptavidin/Biotinylated DNA/Protein Bound Complex Protocol for Determining the Association of c-JUN Protein with NANOG Promoter
Abstract | Full Text: HTML PDF
Unit 2A.10 Isolation of Human Umbilical Cord Blood Aldehyde Dehydrogenase–Expressing Progenitor Cells that Modulate Vascular Regenerative Functions In Vitro and In Vivo
Abstract | Full Text: HTML PDF
Unit 2C.4 Expanding Donor Muscle-Derived Cells for Transplantation
Abstract | Full Text: HTML PDF
Unit 2D.15 Revealing Neuronal Circuitry Using Stem Cell-Derived Neurons
Abstract | Full Text: HTML PDF
Supplement 24, February 2013
Unit 2D.14 Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
Abstract | Full Text: HTML PDF
Unit 3.6 Culture and Isolation of Melanoma-Initiating Cells
Abstract | Full Text: HTML PDF
Unit 3.7 In Vitro Enrichment of Tumor-Initiating Cells from Human Established Cell Lines
Abstract | Full Text: HTML PDF
Supplement 23, November 2012
Unit 1C.13 Adaptation of Stem Cells to 96-Well Plate Assays: Use of Human Embryonic and Mouse Neural Stem Cells in the MTT Assay
Abstract | Full Text: HTML PDF
Unit 1F.13 Serum-Free Generation of Multipotent Mesoderm (Kdr+) Progenitor Cells in Mouse Embryonic Stem Cells for Functional Genomics Screening
Abstract | Full Text: HTML PDF
Unit 2D.13 Functional Stem Cell Integration Assessed by Organotypic Slice Cultures
Abstract | Full Text: HTML PDF
Unit 5B.2 Genetic Manipulation of Human Induced Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 22, August 2012
Unit 1D.8 Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation
Abstract | Full Text: HTML PDF
Unit 1H.6 Dopaminergic Differentiation of Human Pluripotent Cells
Abstract | Full Text: HTML PDF
Unit 2D.12 Scalable Production of Transplantable Dopaminergic Neurons from hESCs and iPSCs in Xeno-Free Defined Conditions
Abstract | Full Text: HTML PDF
Unit 2E.3 Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries
Abstract | Full Text: HTML PDF
Supplement 21, May 2012
Unit 1D.7 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
Abstract | Full Text: HTML PDF
Unit 2D.10 Single and Dual Birthdating Procedures for Assessing the Response of Adult Neural Stem Cells to the Infusion of a Soluble Factor Using Halogenated Thymidine Analogs
Abstract | Full Text: HTML PDF
Unit 2D.11 In Vitro Generation of Three-Dimensional Substrate-Adherent Embryonic Stem Cell–Derived Neural Aggregates for Application in Animal Models of Neurological Disorders
Abstract | Full Text: HTML PDF
Unit 4A.5 Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA
Abstract | Full Text: HTML PDF
Unit 5C.2 High-Efficiency Transfection and siRNA-Mediated Gene Knockdown in Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 20, March 2012
Unit 1D.6 High-Throughput Screening Assay for Embryoid Body Differentiation of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1E.7 A Protocol to Quantify Mammary Early Common Progenitors from Long-Term Mammosphere Culture
Abstract | Full Text: HTML PDF
Unit 1E.8 Amniotic Membrane: Separation of Amniotic Mesoderm from Amniotic Epithelium and Isolation of Their Respective Mesenchymal Stromal and Epithelial Cells
Abstract | Full Text: HTML PDF
Unit 1H.5 Derivation of Cerebellar Neurons from Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 2D.8 Reproducible Expansion and Characterization of Mouse Neural Stem/Progenitor Cells in Adherent Cultures Derived from the Adult Subventricular Zone
Abstract | Full Text: HTML PDF
Unit 2D.9 Neural Differentiation of P19 Carcinoma Cells and Primary Neurospheres: Cell Morphology, Proliferation, Viability, and Functionality
Abstract | Full Text: HTML PDF
Unit 4A.4 Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Supplement 19, December 2011
Unit 1B.9 Quantification of Pluripotency Transcription Factor Levels in Embryonic Stem Cells by Flow Cytometry
Abstract | Full Text: HTML PDF
Unit 1C.12 Live-Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 1H.4 Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells
Abstract | Full Text: HTML PDF
Unit 3.5 Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single-Cell Suspension
Abstract | Full Text: HTML PDF
Supplement 18, September 2011
Unit 1B.8 Identifying Translationally Regulated Genes During Stem Cell Differentiation
Abstract | Full Text: HTML PDF
Unit 2D.7 Neural Stem Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy
Abstract | Full Text: HTML PDF
Unit 4A.3 Derivation of Induced Pluripotent Stem Cells from Human Peripheral Circulating T Cells
Abstract | Full Text: HTML PDF
Unit 5C.1 Generation of Inducible shRNAi Human Embryonic Stem Cell Lines
Abstract | Full Text: HTML PDF
Supplement 17, June 2011
Unit 1F.12 Lentiviral Transduction and Clonal Selection of hESCs with Endothelial-Specific Transgenic Reporters
Abstract | Full Text: HTML PDF
Unit 1H.3 Basic Techniques in Human Mesenchymal Stem Cell Cultures: Differentiation into Osteogenic and Adipogenic Lineages, Genetic Perturbations, and Phenotypic Analyses
Abstract | Full Text: HTML PDF
Unit 3.4 Isolation and and Expansion of Regionally Defined Human Glioblastoma Cells In Vitro
Abstract | Full Text: HTML PDF
Supplement 16, March 2011
Unit 1A.5 Generation of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
Abstract | Full Text: HTML PDF
Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem-Progenitor Cells
Abstract | Full Text: HTML PDF
Supplement 15, December 2010
Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the Hanging-Drop and mass Culture Methods
Abstract | Full Text: HTML PDF
Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere Technique
Abstract | Full Text: HTML PDF
Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian Neocortical Neural Progenitors
Abstract | Full Text: HTML PDF
Supplement 14, September 2010
Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
Abstract | Full Text: HTML PDF
Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
Abstract | Full Text: HTML PDF
Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
Abstract | Full Text: HTML PDF
Unit 1F.10 Isolation and Functional Characterization of Puripotent Stem Cell–Derived Cardiac Progenitor Cells
Abstract | Full Text: HTML PDF
Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
Abstract | Full Text: HTML PDF
Supplement 13, June 2010
Unit 1E.6 Amnion Epithelial cell Isolation and Characterization for Clinical Use
Abstract | Full Text: HTML PDF
Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by Two-Photon Microscopy
Abstract | Full Text: HTML PDF
Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
Abstract | Full Text: HTML PDF
Supplement 12, March 2010
Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
Abstract | Full Text: HTML PDF
Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
Abstract | Full Text: HTML PDF
Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
Abstract | Full Text: HTML PDF
Supplement 11, December 2009
Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
Abstract | Full Text: HTML PDF
Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic Neurons from Rodent Brains
Abstract | Full Text: HTML PDF
Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
Abstract | Full Text: HTML PDF
Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
Abstract | Full Text: HTML PDF
Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by Homologous Recombination
Abstract | Full Text: HTML PDF
Supplement 10, September 2009
Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
Abstract | Full Text: HTML PDF
Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
Abstract | Full Text: HTML PDF
Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage Progression, and Maturation
Abstract | Full Text: HTML PDF
Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
Abstract | Full Text: HTML PDF
Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Supplement 9, June 2009
Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
Abstract | Full Text: HTML PDF
Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
Abstract | Full Text: HTML PDF
Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
Abstract | Full Text: HTML PDF
Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
Abstract | Full Text: HTML PDF
Appendix 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
Abstract | Full Text: HTML PDF
Appendix 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
Abstract | Full Text: HTML PDF
Supplement 8, March 2009
Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
Full Text: HTML PDF
Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
Abstract | Full Text: HTML PDF
Unit 4A.1 Human iPS Cell Derivation/Reprogramming
Abstract | Full Text: HTML PDF
Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
Abstract | Full Text: HTML PDF
Supplement 7, December 2008
Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
Abstract | Full Text: HTML PDF
Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
Abstract | Full Text: HTML PDF
Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
Abstract | Full Text: HTML PDF
Unit 3.1 Colon Cancer Stem Cells
Abstract | Full Text: HTML PDF
Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
Abstract | Full Text: HTML PDF
Supplement 6, September 2008
Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
Abstract | Full Text: HTML PDF
Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
Abstract | Full Text: HTML PDF
Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
Abstract | Full Text: HTML PDF
Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
Abstract | Full Text: HTML PDF
Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
Abstract | Full Text: HTML PDF
Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
Abstract | Full Text: HTML PDF
Abstract | Full Text: HTML PDF
Supplement 5, June 2008
Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
Abstract | Full Text: HTML PDF
Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
Abstract | Full Text: HTML PDF
Unit 1C.4 Culture of Mouse Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1C.6 Isolation of Human Placental Fibroblasts
Abstract | Full Text: HTML PDF
Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
Abstract | Full Text: HTML PDF
Supplement 4, March 2008
Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
Abstract | Full Text: HTML PDF
Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
Abstract | Full Text: HTML PDF
Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues
Abstract | Full Text: HTML PDF
Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
Abstract | Full Text: HTML PDF
Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
Abstract | Full Text: HTML PDF
Supplement 3, December 2007
Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
Abstract | Full Text: HTML PDF
Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
Abstract | Full Text: HTML PDF
Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
Abstract | Full Text: HTML PDF
Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
Full Text: HTML PDF
Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
Abstract | Full Text: HTML PDF
Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
Abstract | Full Text: HTML PDF
Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
Abstract | Full Text: HTML PDF
Supplement 2, September 2007
Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
Abstract | Full Text: HTML PDF
Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
Abstract | Full Text: HTML PDF
Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
Abstract | Full Text: HTML PDF
Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
Abstract | Full Text: HTML PDF
Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum-Free Medium
Abstract | Full Text: HTML PDF
Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
Abstract | Full Text: HTML PDF
Supplement 1, June 2007
Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
Abstract | Full Text: HTML PDF
Unit 1A.2 Derivation of hESC from Intact Blastocysts
Abstract | Full Text: HTML PDF
Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
Abstract | Full Text: HTML PDF
Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
Abstract | Full Text: HTML PDF
Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte-Like Cells
Abstract | Full Text: HTML PDF
Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
Abstract | Full Text: HTML PDF
Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density Gradient
Abstract | Full Text: HTML PDF
Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
Abstract | Full Text: HTML PDF
Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
Abstract | Full Text: HTML PDF
Appendix 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
Full Text: HTML PDF
Appendix 2 Standard Laboratory Equipment
Abstract | Full Text: HTML PDF
