Current Protocols in Stem Cell Biology

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Current Protocols in Stem Cell Biology Supplements

2012:   Supplement 21   Supplement 20   
2011:   Supplement 19   Supplement 18    Supplement 17    Supplement 16   
2010:   Supplement 15   Supplement 14    Supplement 13    Supplement 12   
2009:   Supplement 11   Supplement 10    Supplement 9     Supplement 8    
2008:   Supplement 7     Supplement 6     Supplement 5     Supplement 4    
2007:   Supplement 3     Supplement 2     Supplement 1    

  Supplement 21, May 2012

Unit 1D.7 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
         Abstract | Full Text:  HTML   PDF

Unit 2D.10 Single and Dual Birthdating Procedures for Assessing the Response of Adult Neural Stem Cells to the Infusion of a Soluble Factor Using Halogenated Thymidine Analogs
         Abstract | Full Text:  HTML   PDF

Unit 2D.11 In Vitro Generation of Three-Dimensional Substrate-Adherent Embryonic Stem Cell–Derived Neural Aggregates for Application in Animal Models of Neurological Disorders
         Abstract | Full Text:  HTML   PDF

Unit 4A.5 Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA
         Abstract | Full Text:  HTML   PDF

Unit 5C.2 High-Efficiency Transfection and siRNA-Mediated Gene Knockdown in Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 20, March 2012

Unit 1D.6 High-Throughput Screening Assay for Embryoid Body Differentiation of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.7 A Protocol to Quantify Mammary Early Common Progenitors from Long-Term Mammosphere Culture
         Abstract | Full Text:  HTML   PDF

Unit 1E.8 Amniotic Membrane: Separation of Amniotic Mesoderm from Amniotic Epithelium and Isolation of Their Respective Mesenchymal Stromal and Epithelial Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.5 Derivation of Cerebellar Neurons from Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.8 Reproducible Expansion and Characterization of Mouse Neural Stem/Progenitor Cells in Adherent Cultures Derived from the Adult Subventricular Zone
         Abstract | Full Text:  HTML   PDF

Unit 2D.9 Neural Differentiation of P19 Carcinoma Cells and Primary Neurospheres: Cell Morphology, Proliferation, Viability, and Functionality
         Abstract | Full Text:  HTML   PDF

Unit 4A.4 Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 19, December 2011

Unit 1B.9 Quantification of Pluripotency Transcription Factor Levels in Embryonic Stem Cells by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 1C.12 Live-Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.4 Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.5 Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single-Cell Suspension
         Abstract | Full Text:  HTML   PDF

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  Supplement 18, September 2011

Unit 1B.8 Identifying Translationally Regulated Genes During Stem Cell Differentiation
         Abstract | Full Text:  HTML   PDF

Unit 2D.7 Neural Stem Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy
         Abstract | Full Text:  HTML   PDF

Unit 4A.3 Derivation of Induced Pluripotent Stem Cells from Human Peripheral Circulating T Cells
         Abstract | Full Text:  HTML   PDF

Unit 5C.1 Generation of Inducible shRNAi Human Embryonic Stem Cell Lines
         Abstract | Full Text:  HTML   PDF

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  Supplement 17, June 2011

Unit 1F.12 Lentiviral Transduction and Clonal Selection of hESCs with Endothelial-Specific Transgenic Reporters
         Abstract | Full Text:  HTML   PDF

Unit 1H.3 Basic Techniques in Human Mesenchymal Stem Cell Cultures: Differentiation into Osteogenic and Adipogenic Lineages, Genetic Perturbations, and Phenotypic Analyses
         Abstract | Full Text:  HTML   PDF

Unit 3.4 Isolation and and Expansion of Regionally Defined Human Glioblastoma Cells In Vitro
         Abstract | Full Text:  HTML   PDF

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  Supplement 16, March 2011

Unit 1A.5 Generation of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem-Progenitor Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 15, December 2010

Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the Hanging-Drop and mass Culture Methods
         Abstract | Full Text:  HTML   PDF

Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere Technique
         Abstract | Full Text:  HTML   PDF

Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian Neocortical Neural Progenitors
         Abstract | Full Text:  HTML   PDF

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  Supplement 14, September 2010

Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
         Abstract | Full Text:  HTML   PDF

Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
         Abstract | Full Text:  HTML   PDF

Unit 1F.10 Isolation and Functional Characterization of Puripotent Stem Cell–Derived Cardiac Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
         Abstract | Full Text:  HTML   PDF

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  Supplement 13, June 2010

Unit 1E.6 Amnion Epithelial cell Isolation and Characterization for Clinical Use
         Abstract | Full Text:  HTML   PDF

Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by Two-Photon Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
         Abstract | Full Text:  HTML   PDF

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  Supplement 12, March 2010

Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 11, December 2009

Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
         Abstract | Full Text:  HTML   PDF

Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic Neurons from Rodent Brains
         Abstract | Full Text:  HTML   PDF

Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
         Abstract | Full Text:  HTML   PDF

Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
         Abstract | Full Text:  HTML   PDF

Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by Homologous Recombination
         Abstract | Full Text:  HTML   PDF

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  Supplement 10, September 2009

Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
         Abstract | Full Text:  HTML   PDF

Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
         Abstract | Full Text:  HTML   PDF

Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage Progression, and Maturation
         Abstract | Full Text:  HTML   PDF

Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
         Abstract | Full Text:  HTML   PDF

Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 9, June 2009

Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
         Abstract | Full Text:  HTML   PDF

Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
         Abstract | Full Text:  HTML   PDF

Appendix 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
         Abstract | Full Text:  HTML   PDF

Appendix 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 8, March 2009

Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
         Full Text:  HTML   PDF

Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
         Abstract | Full Text:  HTML   PDF

Unit 4A.1 Human iPS Cell Derivation/Reprogramming
         Abstract | Full Text:  HTML   PDF

Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
         Abstract | Full Text:  HTML   PDF

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  Supplement 7, December 2008

Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
         Abstract | Full Text:  HTML   PDF

Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.1 Colon Cancer Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
         Abstract | Full Text:  HTML   PDF

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  Supplement 6, September 2008

Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
         Abstract | Full Text:  HTML   PDF

Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
         Abstract | Full Text:  HTML   PDF

Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
         Abstract | Full Text:  HTML   PDF

Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
         Abstract | Full Text:  HTML   PDF

Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
         Abstract | Full Text:  HTML   PDF

Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 5, June 2008

Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 1C.4 Culture of Mouse Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.6 Isolation of Human Placental Fibroblasts
         Abstract | Full Text:  HTML   PDF

Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
         Abstract | Full Text:  HTML   PDF

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  Supplement 4, March 2008

Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
         Abstract | Full Text:  HTML   PDF

Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
         Abstract | Full Text:  HTML   PDF

Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues
         Abstract | Full Text:  HTML   PDF

Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 3, December 2007

Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
         Abstract | Full Text:  HTML   PDF

Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
         Abstract | Full Text:  HTML   PDF

Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
         Abstract | Full Text:  HTML   PDF

Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
         Full Text:  HTML   PDF

Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
         Abstract | Full Text:  HTML   PDF

Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
         Abstract | Full Text:  HTML   PDF

Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
         Abstract | Full Text:  HTML   PDF

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  Supplement 2, September 2007

Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
         Abstract | Full Text:  HTML   PDF

Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
         Abstract | Full Text:  HTML   PDF

Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum-Free Medium
         Abstract | Full Text:  HTML   PDF

Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 1, June 2007

Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1A.2 Derivation of hESC from Intact Blastocysts
         Abstract | Full Text:  HTML   PDF

Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
         Abstract | Full Text:  HTML   PDF

Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte-Like Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
         Abstract | Full Text:  HTML   PDF

Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density Gradient
         Abstract | Full Text:  HTML   PDF

Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
         Abstract | Full Text:  HTML   PDF

Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
         Abstract | Full Text:  HTML   PDF

Appendix 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
         Full Text:  HTML   PDF

Appendix 2 Standard Laboratory Equipment
         Abstract | Full Text:  HTML   PDF

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