Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

Online ISBN: 9780470151808

DOI: 10.1002/9780470151808

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Current Protocols in Stem Cell Biology Supplements

2017:   Supplement 40
2016:   Supplement 39     Supplement 38     Supplement 37     Supplement 36  
2015:   Supplement 35     Supplement 34     Supplement 33     Supplement 32  
2014:   Supplement 31     Supplement 30     Supplement 29    Supplement 28    
2013:   Supplement 27     Supplement 26     Supplement 25    Supplement 24    
2012:   Supplement 23     Supplement 22     Supplement 21    Supplement 20    
2011:   Supplement 19     Supplement 18     Supplement 17    Supplement 16    
2010:   Supplement 15     Supplement 14     Supplement 13    Supplement 12    
2009:   Supplement 11     Supplement 10    Supplement 9     Supplement 8    
2008:   Supplement 7     Supplement 6     Supplement 5     Supplement 4    
2007:   Supplement 3     Supplement 2     Supplement 1    




  Supplement 40, February 2017

Unit 1D.9 Purification of Definitive Endoderm Generated from Pluripotent Stem Cells by Magnetic Cell Sorting
         Abstract | Full Text:  HTML   PDF

Unit 1F.16 Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling
         Abstract | Full Text:  HTML   PDF

Unit 2D.19 Magnetic Nanoparticle-Mediated Gene Delivery to Two- and Three-Dimensional Neural Stem Cell Cultures: Magnet-Assisted Transfection and Multifection Approaches to Enhance Outcomes
         Abstract | Full Text:  HTML   PDF

Unit 4A.10 Reprogramming of Pancreatic Acinar Cells to Functional Beta Cells by In Vivo Transduction of a Polycistronic Construct Containing Pdx1, Ngn3, MafA in Mice
         Abstract | Full Text:  HTML   PDF

  Supplement 39, November 2016

Unit 1F.15 Fast and Efficient Neural Conversion of Human Hematopoietic Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.10 Derivation of Telencephalic Oligodendrocyte Progenitors from Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.18 Efficient Generation of Viral and Integration-Free Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes
         Abstract | Full Text:  HTML   PDF


Unit 4A.6 cGMP Generation of Human Induced Pluripotent Stem Cells with Messenger RNA
         Abstract | Full Text:  HTML   PDF

  Supplement 38, August 2016

Unit 1G.6 Small-Molecule-Directed Hepatocyte-Like Cell Differentiation of Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2A.4 Isolation and Assessment of Single Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
         Abstract | Full Text:  HTML   PDF

Unit 2D.7 Neural Stem Cell or Human Induced Pluripotent Stem Cell–Derived GABA-ergic Progenitor Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy
         Abstract | Full Text:  HTML   PDF


Unit 2D.18 Efficient Generation of Viral and Integration-Free Human Induced Pluripotent Stem Cell-Derived Oligodendrocytes
         Abstract | Full Text:  HTML   PDF

Unit 4A.9 Derivation of Transgene-Free Induced Pluripotent Stem Cells from a Single Drop of Blood
         Abstract | Full Text:  HTML   PDF

Unit 5B.5 Self-Cloning CRISPR
         Abstract | Full Text:  HTML   PDF

Unit 5B.6 Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF


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  Supplement 37, May 2016

Unit 1C.19 A Method for Sectioning and Immunohistochemical Analysis of Stem Cell–Derived 3-D Organoids
         Abstract | Full Text:  HTML   PDF

Unit 1E.9 Production of 3-D Airway Organoids From Primary Human Airway Basal Cells and Their Use in High-Throughput Screening
         Abstract | Full Text:  HTML   PDF

Unit 1E.10 Isolation of Human Amnion Epithelial Cells According to Current Good Manufacturing Procedures
         Abstract | Full Text:  HTML   PDF




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  Supplement 36, February 2016

Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.15 Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds
         Abstract | Full Text:  HTML   PDF

Unit 1G.3 Differentiation of Mouse Embryonic Stem Cells into Ventral Foregut Precursors
         Abstract | Full Text:  HTML   PDF

Unit 5A.2 Identifying Adult Stem Cells Using Cre-Mediated Lineage Tracing
         Abstract | Full Text:  HTML   PDF

Unit 5B.4 Delivery of Genome Editing Reagents to Hematopoietic Stem/Progenitor Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 35, November 2015

Unit 1C.14 Standardized Cryopreservation of Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
         Abstract | Full Text:  HTML   PDF

Unit 1E.7 Quantifying Epithelial Early Common Progenitors from Long-Term Primary or Cell Line Sphere Culture
         Abstract | Full Text:  HTML   PDF

Unit 2C.5 The Derivation of Primary Human Epicardium-Derived Cells
         Abstract | Full Text:  HTML   PDF

Unit 5A.8 CRISPR-Mediated Gene Targeting of Human Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 34, August 2015

Unit 2E.3 Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries
         Abstract | Full Text:  HTML   PDF

Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2F.2 Regional Cell Specific RNA Expression Profiling of FACS Isolated Drosophila Intestinal Cell Populations
         Abstract | Full Text:  HTML   PDF

Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
         Abstract | Full Text:  HTML   PDF

Unit 5B.3 TALEN- and CRISPR/Cas9-Mediated Gene Editing in Human Pluripotent Stem Cells Using Lipid-Based Transfection
         Abstract | Full Text:  HTML   PDF



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  Supplement 33, May 2015

Unit 1F.14 Isolation and Expansion of Mesenchymal Stem Cells from Human Conjunctival Tissue
         Abstract | Full Text:  HTML   PDF

Unit 1H.9 Neural Crest Cells from Dual SMAD Inhibition
         Abstract | Full Text:  HTML   PDF



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  Supplement 32, February 2015

Unit 1C.18 Efficient and Scalable Culture of Single Dissociated Human Pluripotent Stem Cells Using Recombinant E8 Fragments of Human Laminin Isoforms
         Abstract | Full Text:  HTML   PDF

Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.8 Generation of Highly Enriched Populations of Optic Vesicle–Like Retinal Cells from Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 4A.8 Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research
         Abstract | Full Text:  HTML   PDF



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  Supplement 31, November 2014

Unit 1A.6 Generation of Mammalian Offspring by Haploid Embryonic Stem Cells Microinjection
         Abstract | Full Text:  HTML   PDF

Unit 1C.17 Cryopreservation of Human Pluripotent Stem Cells in Defined Medium
         Abstract | Full Text:  HTML   PDF

Unit 2D.17 Isolation and Long-Term Expansion of Functional, Myelinating Oligodendrocyte Progenitor Cells from Neonatal Rat Brain
         Abstract | Full Text:  HTML   PDF

Unit 4A.7 Laser-Assisted Generation of Human Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 30, August 2014

Unit 1G.5 Deriving Functional Hepatocytes from Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 29, May 2014

Unit 1C.16 Generation, Expansion, and Differentiation of Human Induced Pluripotent Stem Cells (hiPSCs) Derived From the Umbilical Cords of Newborns
         Abstract | Full Text:  HTML   PDF

Unit 1H.7 Slice Culture Method for Studying Migration of Neuronal Progenitor Cells Derived from Human Embryonic Stem Cells (hESC)
         Abstract | Full Text:  HTML   PDF

Unit 5A.7 Generation of GFP Reporter Human Induced Pluripotent Stem Cells Using AAVS1 Safe Harbor Transcription Activator-Like Effector Nuclease
         Abstract | Full Text:  HTML   PDF



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  Supplement 28, February 2014

Unit 1C.14 Standardized Cryopreservation of Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.15 Expansion of Human Hematopoietic Stem/Progenitor Cells on Decellularized Matrix Scaffolds
         Abstract | Full Text:  HTML   PDF

Unit 2B.6 Preparation of Anti-Inflammatory Mesenchymal Stem/Precursor Cells (MSCs) Through Sphere Formation Using Hanging-Drop Culture Technique
         Abstract | Full Text:  HTML   PDF



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  Supplement 27, November 2013

Unit 2B.5 Preparation and Osteogenic Differentiation of Scaffold-Free Mouse Adipose-Derived Stromal Cell Microtissue Spheroids (ASC-MT)
         Abstract | Full Text:  HTML   PDF

Unit 2F.2 RNA Expression Profiling from FACS-Isolated Cells of the Drosophila Intestine
         Abstract | Full Text:  HTML   PDF

Unit 4A.6 Feeder-Free Reprogramming of Human Fibroblasts with Messenger RNA
         Abstract | Full Text:  HTML   PDF

Unit 5A.6 Retroviral Gene Expression Control in Primary Organoid Cultures
         Abstract | Full Text:  HTML   PDF



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  Supplement 26, August 2013

Unit 1G.4 Differentiation of Hepatocytes from Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2B.4 Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
         Abstract | Full Text:  HTML   PDF

Unit 2D.16 Intraspinal Transplantation of Mouse and Human Neural Precursor Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.8 Isolation of Human Melanoma Stem Cells Using ALDH as a Marker
         Abstract | Full Text:  HTML   PDF

Unit 5C.3 Array-Based High-Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
         Abstract | Full Text:  HTML   PDF



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  Supplement 25, May 2013

Unit 1B.10 The Streptavidin/Biotinylated DNA/Protein Bound Complex Protocol for Determining the Association of c-JUN Protein with NANOG Promoter
         Abstract | Full Text:  HTML   PDF

Unit 2A.10 Isolation of Human Umbilical Cord Blood Aldehyde Dehydrogenase–Expressing Progenitor Cells that Modulate Vascular Regenerative Functions In Vitro and In Vivo
         Abstract | Full Text:  HTML   PDF

Unit 2C.4 Expanding Donor Muscle-Derived Cells for Transplantation
         Abstract | Full Text:  HTML   PDF

Unit 2D.15 Revealing Neuronal Circuitry Using Stem Cell-Derived Neurons
         Abstract | Full Text:  HTML   PDF



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  Supplement 24, February 2013

Unit 2D.14 Evaluation of Proliferation of Neural Stem Cells In Vitro and In Vivo
         Abstract | Full Text:  HTML   PDF

Unit 3.6 Culture and Isolation of Melanoma-Initiating Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.7 In Vitro Enrichment of Tumor-Initiating Cells from Human Established Cell Lines
         Abstract | Full Text:  HTML   PDF



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  Supplement 23, November 2012

Unit 1C.13 Adaptation of Stem Cells to 96-Well Plate Assays: Use of Human Embryonic and Mouse Neural Stem Cells in the MTT Assay
         Abstract | Full Text:  HTML   PDF

Unit 1F.13 Serum-Free Generation of Multipotent Mesoderm (Kdr+) Progenitor Cells in Mouse Embryonic Stem Cells for Functional Genomics Screening
         Abstract | Full Text:  HTML   PDF

Unit 2D.13 Functional Stem Cell Integration Assessed by Organotypic Slice Cultures
         Abstract | Full Text:  HTML   PDF

Unit 5B.2 Genetic Manipulation of Human Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 22, August 2012

Unit 1D.8 Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation
         Abstract | Full Text:  HTML   PDF

Unit 1H.6 Dopaminergic Differentiation of Human Pluripotent Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.12 Scalable Production of Transplantable Dopaminergic Neurons from hESCs and iPSCs in Xeno-Free Defined Conditions
         Abstract | Full Text:  HTML   PDF

Unit 2E.3 Isolation of Undifferentiated Female Germline Cells from Adult Drosophila Ovaries
         Abstract | Full Text:  HTML   PDF



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  Supplement 21, May 2012

Unit 1D.7 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
         Abstract | Full Text:  HTML   PDF

Unit 2D.10 Single and Dual Birthdating Procedures for Assessing the Response of Adult Neural Stem Cells to the Infusion of a Soluble Factor Using Halogenated Thymidine Analogs
         Abstract | Full Text:  HTML   PDF

Unit 2D.11 In Vitro Generation of Three-Dimensional Substrate-Adherent Embryonic Stem Cell–Derived Neural Aggregates for Application in Animal Models of Neurological Disorders
         Abstract | Full Text:  HTML   PDF

Unit 4A.5 Excision of a Viral Reprogramming Cassette by Delivery of Synthetic Cre mRNA
         Abstract | Full Text:  HTML   PDF

Unit 5C.2 High-Efficiency Transfection and siRNA-Mediated Gene Knockdown in Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 20, March 2012

Unit 1D.6 High-Throughput Screening Assay for Embryoid Body Differentiation of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.7 A Protocol to Quantify Mammary Early Common Progenitors from Long-Term Mammosphere Culture
         Abstract | Full Text:  HTML   PDF

Unit 1E.8 Amniotic Membrane: Separation of Amniotic Mesoderm from Amniotic Epithelium and Isolation of Their Respective Mesenchymal Stromal and Epithelial Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.5 Derivation of Cerebellar Neurons from Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.8 Reproducible Expansion and Characterization of Mouse Neural Stem/Progenitor Cells in Adherent Cultures Derived from the Adult Subventricular Zone
         Abstract | Full Text:  HTML   PDF

Unit 2D.9 Neural Differentiation of P19 Carcinoma Cells and Primary Neurospheres: Cell Morphology, Proliferation, Viability, and Functionality
         Abstract | Full Text:  HTML   PDF

Unit 4A.4 Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 19, December 2011

Unit 1B.9 Quantification of Pluripotency Transcription Factor Levels in Embryonic Stem Cells by Flow Cytometry
         Abstract | Full Text:  HTML   PDF

Unit 1C.12 Live-Cell Immunofluorescence Staining of Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.4 Isolation and Culture of Adult Ciliary Epithelial Cells, Previously Identified as Retinal Stem Cells, and Retinal Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.5 Organotypic Explant Culture of Glioblastoma Multiforme and Subsequent Single-Cell Suspension
         Abstract | Full Text:  HTML   PDF



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  Supplement 18, September 2011

Unit 1B.8 Identifying Translationally Regulated Genes During Stem Cell Differentiation
         Abstract | Full Text:  HTML   PDF

Unit 2D.7 Neural Stem Cell Grafting in an Animal Model of Chronic Temporal Lobe Epilepsy
         Abstract | Full Text:  HTML   PDF

Unit 4A.3 Derivation of Induced Pluripotent Stem Cells from Human Peripheral Circulating T Cells
         Abstract | Full Text:  HTML   PDF

Unit 5C.1 Generation of Inducible shRNAi Human Embryonic Stem Cell Lines
         Abstract | Full Text:  HTML   PDF



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  Supplement 17, June 2011

Unit 1F.12 Lentiviral Transduction and Clonal Selection of hESCs with Endothelial-Specific Transgenic Reporters
         Abstract | Full Text:  HTML   PDF

Unit 1H.3 Basic Techniques in Human Mesenchymal Stem Cell Cultures: Differentiation into Osteogenic and Adipogenic Lineages, Genetic Perturbations, and Phenotypic Analyses
         Abstract | Full Text:  HTML   PDF

Unit 3.4 Isolation and and Expansion of Regionally Defined Human Glioblastoma Cells In Vitro
         Abstract | Full Text:  HTML   PDF



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  Supplement 16, March 2011

Unit 1A.5 Generation of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2C.3 Isolation and Expansion of Cardiosphere-Derived Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2G.1 Isolation and Clonal Assay of Adult Lung Epithelial Stem-Progenitor Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 15, December 2010

Unit 1F.11 Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the Hanging-Drop and mass Culture Methods
         Abstract | Full Text:  HTML   PDF

Unit 2D.6 Isolation of Neural Stem Cells from Neural Tissues Using the Neurosphere Technique
         Abstract | Full Text:  HTML   PDF

Unit 5A.5 Kaede-Centrin1 Labeling of Mother and Daughter Centrosomes in Mammalian Neocortical Neural Progenitors
         Abstract | Full Text:  HTML   PDF



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  Supplement 14, September 2010

Unit 1B.7 Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
         Abstract | Full Text:  HTML   PDF

Unit 1C.10 Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.11 Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers
         Abstract | Full Text:  HTML   PDF

Unit 1F.10 Isolation and Functional Characterization of Puripotent Stem Cell–Derived Cardiac Progenitor Cells
         Abstract | Full Text:  HTML   PDF

Unit 2A.9 Preparation of Hematopoietic Stem and Progenitor Cells from the Human Placenta
         Abstract | Full Text:  HTML   PDF



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  Supplement 13, June 2010

Unit 1E.6 Amnion Epithelial cell Isolation and Characterization for Clinical Use
         Abstract | Full Text:  HTML   PDF

Unit 1H.2 Time-Lapse Imaging of Embryonic Neural Stem Cell Division in Drosophila by Two-Photon Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 5A.4 Lineage Tracing in the Intestinal Epithelium
         Abstract | Full Text:  HTML   PDF



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  Supplement 12, March 2010

Unit 1B.6 Characterization of X-Chromosome Inactivation Status in Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.5 Isolation of Amniotic Mesenchymal Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.9 Derivation of Vasculature from Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2F.1 In Situ Hybridization to Identify Gut Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 11, December 2009

Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
         Abstract | Full Text:  HTML   PDF

Unit 1C.9 Authentication and Banking of Human Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.5 Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic Neurons from Rodent Brains
         Abstract | Full Text:  HTML   PDF

Unit 2E.2 Time-Lapse Live Imaging of Stem Cells in Drosophila Testis
         Abstract | Full Text:  HTML   PDF

Unit 3.3 Culture and Isolation of Brain Tumor Initiating Cells
         Abstract | Full Text:  HTML   PDF

Unit 5B.1 Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by Homologous Recombination
         Abstract | Full Text:  HTML   PDF



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  Supplement 10, September 2009

Unit 1C.8 Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
         Abstract | Full Text:  HTML   PDF

Unit 1G.3 Differentiation of Embryonic Stem Cells into Anterior Definitive Endoderm
         Abstract | Full Text:  HTML   PDF

Unit 2D.4 Culture System for Rodent and Human Oligodendrocyte Specification, Lineage Progression, and Maturation
         Abstract | Full Text:  HTML   PDF

Unit 2E.1 Culturing Ovarian Somatic and Germline Stem Cells of Drosophila
         Abstract | Full Text:  HTML   PDF

Unit 5A.3 Magnetic Resonance Imaging of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 9, June 2009

Unit 1D.5 Isolation and Differentiation of Xenopus Animal Cap Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.8 Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2D.3 Long-Term Multilayer Adherent Network (MAN) Expansion, Maintenance, and Characterization, Chemical and Genetic Manipulation, and Transplantation of Human Fetal Forebrain Neural Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 4A.2 Generation and Characterization of Human Induced Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 4B.1 Heterokaryon-Based Reprogramming for Pluripotency
         Abstract | Full Text:  HTML   PDF

Unit 5A.2 Functional Analysis of Adult Stem Cells Using Cre-Mediated Lineage Tracing
         Abstract | Full Text:  HTML   PDF

Appendix 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
         Abstract | Full Text:  HTML   PDF

Appendix 1B ISSCR Guidelines for the Clinical Translation of Stem Cells
         Abstract | Full Text:  HTML   PDF



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  Supplement 8, March 2009

Unit 1B.5 Tandem Affinity Purification of Protein Complexes in Mouse Embryonic Stem Cells Using In Vivo Biotinylation
         Full Text:  HTML   PDF

Unit 2C.2 Derivation of Epicardium-Derived Progenitor Cells (EPDCs) from Adult Epicardium
         Abstract | Full Text:  HTML   PDF

Unit 4A.1 Human iPS Cell Derivation/Reprogramming
         Abstract | Full Text:  HTML   PDF

Unit 5A.1 Imaging Neural Stem Cell Fate in Mouse Model of Glioma
         Abstract | Full Text:  HTML   PDF


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  Supplement 7, December 2008

Unit 1E.4 Isolation and Manipulation of Mouse Trophoblast Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.7 TLX1 (HOX11) Immortalization of Embryonic Stem Cell–Derived and Primary Murine Hematopoietic Progenitors
         Abstract | Full Text:  HTML   PDF

Unit 2B.3 Isolation, Culture, and Differentiation Potential of Mouse Marrow Stromal Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.1 Colon Cancer Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 3.2 In Vivo Evaluation of Leukemic Stem Cells through the Xenotransplantation Model
         Abstract | Full Text:  HTML   PDF


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  Supplement 6, September 2008

Unit 1A.4 Derivation and Propagation of hESC Under a Therapeutic Environment
         Abstract | Full Text:  HTML   PDF

Unit 1F.4 Differentiation of Mouse Embryonic Stem Cells into Blood
         Abstract | Full Text:  HTML   PDF

Unit 1F.5 Endothelial Differentiation of Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.6 Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
         Abstract | Full Text:  HTML   PDF

Unit 2A.8 Isolation and Visualization of Mouse Placental Hematopoietic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2C.1 Isolation and Characterization of Endothelial Progenitor Cells from Human Blood
         Abstract | Full Text:  HTML   PDF

Unit 2D.1 Generation of Cultured Oligodendrocyte Progenitor Cells from Rat Neonatal Brains
         Abstract | Full Text:  HTML   PDF

Unit 2D.2 Isolating, Expanding, and Infecting Human and Rodent Fetal Neural Progenitor Cells
         Abstract | Full Text:  HTML   PDF


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  Supplement 5, June 2008

Unit 1A.3 Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 1C.4 Culture of Mouse Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.6 Isolation of Human Placental Fibroblasts
         Abstract | Full Text:  HTML   PDF

Unit 1C.7 Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1H.1 Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
         Abstract | Full Text:  HTML   PDF

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  Supplement 4, March 2008

Unit 1C.5 Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1D.3 Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
         Abstract | Full Text:  HTML   PDF

Unit 1D.4 Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
         Abstract | Full Text:  HTML   PDF

Unit 1F.3 Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2A.6 Analysis and Manipulation of Hematopoietic Progenitor and Stem Cells from Murine Embryonic Tissues
         Abstract | Full Text:  HTML   PDF

Unit 2A.7 High Level In Vitro Expansion of Murine Hematopoietic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 2B.2 Purification and Culture of Human Blood Vessel–Associated Progenitor Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 3, December 2007

Unit 1B.4 Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
         Abstract | Full Text:  HTML   PDF

Unit 1C.3 Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
         Abstract | Full Text:  HTML   PDF

Unit 1D.2 Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
         Abstract | Full Text:  HTML   PDF

Unit 1E.3 Isolation of Amniotic Epithelial Stem Cells
         Full Text:  HTML   PDF

Unit 2A.4 Isolation and Assessment of Long-Term Reconstituting Hematopoietic Stem Cells from Adult Mouse Bone Marrow
         Abstract | Full Text:  HTML   PDF

Unit 2A.5 Analysis of the Hematopoietic Stem Cell Niche
         Abstract | Full Text:  HTML   PDF

Unit 2B.1 Isolation and Characterization of Mesoangioblasts from Mouse, Dog, and Human Tissues
         Abstract | Full Text:  HTML   PDF

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  Supplement 2, September 2007

Unit 1B.1 Proteomic Analysis of Pluripotent Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1B.2 Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
         Abstract | Full Text:  HTML   PDF

Unit 1B.3 Phenotypic Analysis of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1C.2 Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
         Abstract | Full Text:  HTML   PDF

Unit 1F.1 Differentiation of Embryonic Stem Cells into Cartilage Cells
         Abstract | Full Text:  HTML   PDF

Unit 1F.2 Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum-Free Medium
         Abstract | Full Text:  HTML   PDF

Unit 1G.1 The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1G.2 Pancreas Differentiation of Mouse ES Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 1, June 2007

Unit 1A.1 Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF

Unit 1A.2 Derivation of hESC from Intact Blastocysts
         Abstract | Full Text:  HTML   PDF

Unit 1C.1 Expansion of Human Embryonic Stem Cells In Vitro
         Abstract | Full Text:  HTML   PDF

Unit 1D.1 Germ Layer Induction in ESC—Following the Vertebrate Roadmap
         Abstract | Full Text:  HTML   PDF

Unit 1E.1 Isolation of Human Placenta-Derived Multipotent Cells and In Vitro Differentiation into Hepatocyte-Like Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.2 Isolation of Mesenchymal Stem Cells from Amniotic Fluid and Placenta
         Abstract | Full Text:  HTML   PDF

Unit 2A.1 Isolation of Mononuclear Cells from Human Cord Blood by Ficoll-Paque Density Gradient
         Abstract | Full Text:  HTML   PDF

Unit 2A.2 Isolation of Hematopoietic Stem Cells from Human Cord Blood
         Abstract | Full Text:  HTML   PDF

Unit 2A.3 Isolation of Mesenchymal Stem Cells from Human Cord Blood
         Abstract | Full Text:  HTML   PDF

Appendix 1A Guidelines for the Conduct of Human Embryonic Stem Cell Research
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Appendix 2 Standard Laboratory Equipment
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