Current Protocols in Stem Cell Biology

Current Protocols in Stem Cell Biology

Online ISBN: 9780470151808

DOI: 10.1002/9780470151808

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Culture
  4. Cytometry
  5. Gene Expression
  6. Genetics and Genomics
  7. Imaging and Microscopy
  8. Immunology
  9. Intermolecular Interactions
  10. Laboratory Organisms/Animal Models
  11. Magnetic Resonance Imaging
  12. Molecular Biology
  13. Mouse Biology
  14. Neuroscience
  15. Nucleic Acid Chemistry
  16. Pharmacology/Drug Discovery
  17. Protein Production, Purification, and Analysis
  18. Proteomics
  19. RNA
  20. Stem cells
    1. Cancer Stem Cells
    2. Characterization
    3. Characterization
    4. Culture
    5. Differentiation
    6. Ectoderm
    7. Embryonic Stem Cells
      1. Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation
      2. Array-Based High-Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
      3. Authentication and Banking of Human Pluripotent Stem Cells
      4. Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
      5. Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
      6. Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
      7. Cryopreservation of Human Pluripotent Stem Cells in Defined Medium
      8. Culture of Mouse Embryonic Stem Cells
      9. Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
      10. Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
      11. Derivation and Propagation of hESC Under a Therapeutic Environment
      12. Derivation of Cerebellar Neurons from Human Pluripotent Stem Cells
      13. Derivation of hESC from Intact Blastocysts
      14. Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
      15. Derivation of Vasculature from Embryonic Stem Cells
      16. The Streptavidin/Biotinylated DNA/Protein Bound Complex Protocol for Determining the Association of c-JUN Protein with NANOG Promoter
      17. The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
      18. Differentiation of Embryonic Stem Cells into Cartilage Cells
      19. Differentiation of Hepatocytes from Pluripotent Stem Cells
      20. Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
      21. Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum-Free Medium
      22. Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
      23. Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
      24. Differentiation of Mouse Embryonic Stem Cells into Blood
      25. Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the Hanging-Drop and Mass Culture Methods
      26. Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
      27. Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
      28. Dopaminergic Differentiation of Human Pluripotent Cells
      29. Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling
      30. Endothelial Differentiation of Embryonic Stem Cells
      31. Expansion of Human Embryonic Stem Cells In Vitro
      32. Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
      33. Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by Homologous Recombination
      34. Generation of Inducible shRNAi Human Embryonic Stem Cell Lines
      35. Generation of Mammalian Offspring by Haploid Embryonic Stem Cells Microinjection
      36. Genetic Manipulation of Human Induced Pluripotent Stem Cells
      37. Germ Layer Induction in ESC—Following the Vertebrate Roadmap
      38. Guidelines for the Conduct of Human Embryonic Stem Cell Research
      39. Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
      40. Heterokaryon-Based Reprogramming for Pluripotency
      41. In Vitro Generation of Three-Dimensional Substrate-Adherent Embryonic Stem Cell–Derived Neural Aggregates for Application in Animal Models of Neurological Disorders
      42. Derivation of Induced Pluripotent Stem Cells from Human Peripheral Circulating T Cells
      43. Isolation and Differentiation of Xenopus Animal Cap Cells
      44. Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac Progenitor Cells
      45. Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
      46. Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
      47. Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
      48. Isolation of Human Placental Fibroblasts
      49. ISSCR Guidelines for the Clinical Translation of Stem Cells
      50. Magnetic Resonance Imaging of Human Embryonic Stem Cells
      51. Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
      52. Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
      53. Adaptation of Stem Cells to 96-Well Plate Assays: Use of Human Embryonic and Mouse Neural Stem Cells in the MTT Assay
      54. Pancreas Differentiation of Mouse ES Cells
      55. Phenotypic Analysis of Human Embryonic Stem Cells
      56. Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
      57. Quantification of Pluripotency Transcription Factor Levels in Embryonic Stem Cells by Flow Cytometry
      58. Reprogramming of Pancreatic Acinar Cells to Functional Beta Cells by In Vivo Transduction of a Polycistronic Construct Containing Pdx1, Ngn3, MafA in Mice
      59. Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
      60. Revealing Neuronal Circuitry Using Stem Cell-Derived Neurons
      61. Scalable Production of Transplantable Dopaminergic Neurons from hESCs and iPSCs in Xeno-Free Defined Conditions
      62. Serum-Free Generation of Multipotent Mesoderm (Kdr+) Progenitor Cells in Mouse Embryonic Stem Cells for Functional Genomics Screening
      63. Standardized Cryopreservation of Pluripotent Stem Cells
      64. Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research
      65. Lentiviral Transduction and Clonal Selection of hESCs with Endothelial-Specific Transgenic Reporters
      66. Using ES Cells Labeled with GFP for Analyzing Cell Behavior During Differentiation
      67. Array-Based High-Throughput Screening in Mouse Embryonic Stem Cells with shRNAs
      68. Authentication and Banking of Human Pluripotent Stem Cells
      69. Preparation of Autogenic Human Feeder Cells for Growth of Human Embryonic Stem Cells
      70. Clump Passaging and Expansion of Human Embryonic and Induced Pluripotent Stem Cells on Mouse Embryonic Fibroblast Feeder Cells
      71. Cryopreservation of Dissociated Human Embryonic Stem Cells in the Presence of ROCK Inhibitor
      72. Cryopreservation of Human Pluripotent Stem Cells in Defined Medium
      73. Culture of Mouse Embryonic Stem Cells
      74. Defined, Feeder-Independent Medium for Human Embryonic Stem Cell Culture
      75. Derivation and Characterization of Nonhuman Primate Embryonic Stem Cells
      76. Derivation and Propagation of hESC Under a Therapeutic Environment
      77. Derivation of Cerebellar Neurons from Human Pluripotent Stem Cells
      78. Derivation of hESC from Intact Blastocysts
      79. Derivation of Human Skin Fibroblast Lines for Feeder Cells of Human Embryonic Stem Cells
      80. Derivation of Vasculature from Embryonic Stem Cells
      81. The Streptavidin/Biotinylated DNA/Protein Bound Complex Protocol for Determining the Association of c-JUN Protein with NANOG Promoter
      82. The Differentiation of Distal Lung Epithelium from Embryonic Stem Cells
      83. Differentiation of Embryonic Stem Cells into Cartilage Cells
      84. Differentiation of Hepatocytes from Pluripotent Stem Cells
      85. Differentiation of Human Embryonic Stem Cells in Adherent and in Chemically Defined Culture Conditions
      86. Differentiation of Human Embryonic Stem Cells to Cardiomyocytes by Coculture with Endoderm in Serum-Free Medium
      87. Differentiation of Human Embryonic Stem Cells to Cardiomyocytes on Microcarrier Cultures
      88. Differentiation of Mouse Embryonic Stem Cells to Spinal Motor Neurons
      89. Differentiation of Mouse Embryonic Stem Cells into Blood
      90. Differentiation of Mouse Embryonic Stem Cells into Cardiomyocytes via the Hanging-Drop and Mass Culture Methods
      91. Differentiation of Multipotent Mesenchymal Precursors and Skeletal Myoblasts from Human Embryonic Stem Cells
      92. Directed Differentiation of Human Embryonic Stem Cells as Spin Embryoid Bodies and a Description of the Hematopoietic Blast Colony Forming Assay
      93. Dopaminergic Differentiation of Human Pluripotent Cells
      94. Efficient Generation of Cardiac Purkinje-like Cells from Embryonic Stem Cells by Activating cAMP Signaling
      95. Endothelial Differentiation of Embryonic Stem Cells
      96. Expansion of Human Embryonic Stem Cells In Vitro
      97. Formation and Hematopoietic Differentiation of Human Embryoid Bodies by Suspension and Hanging Drop Cultures
      98. Generation of Human Embryonic Stem Cell Reporter Knock-In Lines by Homologous Recombination
      99. Generation of Inducible shRNAi Human Embryonic Stem Cell Lines
      100. Generation of Mammalian Offspring by Haploid Embryonic Stem Cells Microinjection
      101. Genetic Manipulation of Human Induced Pluripotent Stem Cells
      102. Germ Layer Induction in ESC—Following the Vertebrate Roadmap
      103. Guidelines for the Conduct of Human Embryonic Stem Cell Research
      104. Hematopoietic Differentiation of Human Embryonic Stem Cells by Cocultivation with Stromal Layers
      105. Heterokaryon-Based Reprogramming for Pluripotency
      106. In Vitro Generation of Three-Dimensional Substrate-Adherent Embryonic Stem Cell–Derived Neural Aggregates for Application in Animal Models of Neurological Disorders
      107. Derivation of Induced Pluripotent Stem Cells from Human Peripheral Circulating T Cells
      108. Isolation and Differentiation of Xenopus Animal Cap Cells
      109. Isolation and Functional Characterization of Pluripotent Stem Cell–Derived Cardiac Progenitor Cells
      110. Isolation and Propagation of Mouse Embryonic Fibroblasts and Preparation of Mouse Embryonic Feeder Layer Cells
      111. Isolation of Human Embryonic Stem Cell–Derived Teratomas for the Assessment of Pluripotency
      112. Isolation of Hematopoietic Stem Cells from Mouse Embryonic Stem Cells
      113. Isolation of Human Placental Fibroblasts
      114. ISSCR Guidelines for the Clinical Translation of Stem Cells
      115. Magnetic Resonance Imaging of Human Embryonic Stem Cells
      116. Gene Expression Analysis of RNA Purified from Embryonic Stem Cells and Embryoid Body–Derived Cells Using a High-Throughput Microarray Platform
      117. Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
      118. Adaptation of Stem Cells to 96-Well Plate Assays: Use of Human Embryonic and Mouse Neural Stem Cells in the MTT Assay
      119. Pancreas Differentiation of Mouse ES Cells
      120. Phenotypic Analysis of Human Embryonic Stem Cells
      121. Preparation of Defined Human Embryonic Stem Cell Populations for Transcriptional Profiling
      122. Quantification of Pluripotency Transcription Factor Levels in Embryonic Stem Cells by Flow Cytometry
      123. Reprogramming of Pancreatic Acinar Cells to Functional Beta Cells by In Vivo Transduction of a Polycistronic Construct Containing Pdx1, Ngn3, MafA in Mice
      124. Reprogramming Primordial Germ Cells (PGC) to Embryonic Germ (EG) Cells
      125. Revealing Neuronal Circuitry Using Stem Cell-Derived Neurons
      126. Scalable Production of Transplantable Dopaminergic Neurons from hESCs and iPSCs in Xeno-Free Defined Conditions
      127. Serum-Free Generation of Multipotent Mesoderm (Kdr+) Progenitor Cells in Mouse Embryonic Stem Cells for Functional Genomics Screening
      128. Standardized Cryopreservation of Pluripotent Stem Cells
      129. Teratoma Formation: A Tool for Monitoring Pluripotency in Stem Cell Research
      130. Lentiviral Transduction and Clonal Selection of hESCs with Endothelial-Specific Transgenic Reporters
    8. Endoderm
    9. Extra embryonic stem cells
    10. Genetic Manipulation
    11. Germline
    12. Hematopoiesis
    13. Isolation
    14. Manipulation of Potency
    15. Manipulaton of Potency
    16. Mesoderm
    17. Somatic stem cells
  21. Stem Cells
  22. Supporting Lab Techniques
  23. Toxicology

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