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Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

Online ISBN: 9780470559277

DOI: 10.1002/9780470559277

Browse by Table of Contents

  1. Volume 4
    1. Issue 1
      1. Gene Assembly from Chip-Synthesized Oligonucleotides
      2. Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases with Clickable NAD Analog
      3. On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands
      4. High-Throughput RT-PCR for Small-Molecule Screening Assays
      5. Measurement of Protein Phosphorylation Stoichiometry by SRM-MS
      6. Kinase-Catalyzed Biotinylation of Peptides, Proteins, and Lysates
  2. Volume 3
    1. Issue 1
      1. Parallel High-Throughput Automated Assays to Measure Cell Growth and Beta-Galactosidase Reporter Gene Expression in the Yeast Saccharomyces cerevisiae
      2. Development of Chemical Probes for Biochemical Detection and Cellular Imaging of Myristoylated and Palmitoylated Proteins
      3. Imaging of Endogenous RNA Using Genetically Encoded Probes
      4. Development of In-Cell Western Assays Using Far-Red Fluorophores
    2. Issue 2
      1. Derivatization of Free Natural Glycans for Incorporation onto Glycan Arrays: Derivatizing Glycans on the Microscale for Microarray and Other Applications
      2. Visualization and Identification of Fatty Acylated Proteins Using Chemical Reporters
      3. Miniaturized High-Throughput Fluorescent Assay for Conversion of NAD(P)H to NAD(P)
    3. Issue 3
      1. Chemical Synthesis of Hydrocarbon-Stapled Peptides for Protein Interaction Research and Therapeutic Targeting
      2. Triple-Addition Assay Protocols for Detecting and Characterizing Modulators of Seven-Transmembrane Receptors
      3. Compound Management: Guidelines for Compound Storage, Provision, and Quality Control
    4. Issue 4
      1. Copper-Catalyzed Azide–Alkyne Click Chemistry for Bioconjugation
      2. Target Identification Using Drug Affinity Responsive Target Stability (DARTS)
      3. Analyzing In Vivo Metabolite-Protein Interactions by Large-Scale Systematic Analyses
  3. Volume 2
    1. Issue 1
      1. Cross-Coupling Modification of Nucleoside Triphosphates, PEX, and PCR Construction of Base-Modified DNA
      2. Chemical Genetic Approach for Kinase-Substrate Mapping by Covalent Capture of Thiophosphopeptides and Analysis by Mass Spectrometry
      3. Construction and Use of Glycan Microarrays
    2. Issue 2
      1. Quantitative Glycomics of Cultured Cells Using Isotopic Detection of Aminosugars with Glutamine (IDAWG)
      2. Targeted Degradation of Proteins by PROTACs
      3. Directed Evolution of DNA Polymerases: Construction and Screening of DNA Polymerase Mutant Libraries
      4. Nucleotide Analogues as Probes for DNA and RNA Polymerases
      5. Site-Specific Protein Bioconjugation via a Pyridoxal 5¢-Phosphate-Mediated N-Terminal Transamination Reaction
    3. Issue 3
      1. Small-Molecule Library Synthesis on Silicon-Functionalized SynPhase Lanterns
      2. High-Throughput Assessment of Mammalian Cell Viability by Determination of Adenosine Triphosphate Levels
      3. Preparation, Characterization, and Application of Optical Switch Probes
      4. Experimental Design Considerations for In Vitro Non-Natural Glycan Display via Metabolic Oligosaccharide Engineering
    4. Issue 4
      1. High-Throughput Assessment of Bacterial Growth Inhibition by Optical Density Measurements
      2. Mass Spectrometry-Based Identification of Protein Kinase Substrates Utilizing Engineered Kinases and Thiophosphate Labeling
      3. Supported Membrane Formation, Characterization, Functionalization, and Patterning for Application in Biological Science and Technology
  4. Volume 1
    1. Issue 1
      1. Fluorescence Polarization (FP) Assays for Monitoring Peptide-Protein or Nucleic Acid-Protein Binding
      2. Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters
      3. Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)–ABPP by MudPIT Mass Spectrometry
      4. Overview of Liquid Handling Instrumentation for High-Throughput Screening Applications
      5. Target Identification by Diazirine Photo-Cross-Linking and Click Chemistry

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