Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

Online ISBN: 9780470559277

DOI: 10.1002/9780470559277

Browse by Topic

  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Cell Culture
  6. Chemical Biology
  7. Chemical Biology
    1. Carbohydrate Modifications, Engineering and Applications
    2. Chemical Tools for Probing Small Molecule-Macromolecule Interactions
    3. Enzyme Engineering
    4. General Methods in Chemical Biology
    5. High-Content Assay Design
    6. High-Throughput Assay Design
    7. Lipid Modifications, Engineering and Applications
    8. Nucleic Acid Modifications, Engineering and Applications
    9. Protein Modification and Labeling
      1. A Biocompatible “Split Luciferin” Reaction and Its Application for Non-Invasive Bioluminescent Imaging of Protease Activity in Living Animals
      2. Biochemical, Biophysical and Cellular Techniques to Study the Guanine Nucleotide Exchange Factor, GIV/Girdin
      3. Bioorthogonal Profiling of Protein Methylation (BPPM) Using an Azido Analog of S-Adenosyl-L-Methionine
      4. Bioorthogonal Chemical Reporters for Analyzing Protein Sulfenylation in Cells
      5. Activity-Based Protein Profiling (ABPP) and Click Chemistry (CC)–ABPP by MudPIT Mass Spectrometry
      6. Spatiotemporal Control of Gene Expression in Mammalian Cells and in Mice Using the LightOn System
      7. Genetic Code Expansion of Mammalian Cells with Unnatural Amino Acids
      8. High-Throughput Phosphoproteomics from Formalin-Fixed, Paraffin-Embedded Tissues
      9. Identifying Direct Protein Targets of Poly-ADP-Ribose Polymerases (PARPs) Using Engineered PARP Variants—Orthogonal Nicotinamide Adenine Dinucleotide (NAD+) Analog Pairs
      10. Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters
      11. Chemical Genetic Approach for Kinase-Substrate Mapping by Covalent Capture of Thiophosphopeptides and Analysis by Mass Spectrometry
      12. Labeling Substrate Proteins of Poly(ADP-ribose) Polymerases with Clickable NAD Analog
      13. Local Generation and Imaging of Hydrogen Peroxide in Living Cells
      14. Molecular Evolution Directs Protein Translation Using Unnatural Amino Acids
      15. Experimental Design Considerations for In Vitro Non-Natural Glycan Display via Metabolic Oligosaccharide Engineering
      16. Preparation, Characterization, and Application of Optical Switch Probes
      17. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS)
      18. Penicillin-Binding Protein Imaging Probes
      19. Phage Selection of Peptide “Microantibodies”
      20. Photoactivated In Vivo Proximity Labeling
      21. Profiling Native Kinases by Immuno-Assisted Activity-Based Profiling
      22. Profiling Protein Methylation with Cofactor Analog Containing Terminal Alkyne Functionality
      23. In Vivo Imaging and Biochemical Characterization of Protease Function Using Fluorescent Activity-Based Probes
      24. Measurement of Protein Phosphorylation Stoichiometry by SRM-MS
      25. Quantification of Protein Kinase Enzymatic Activity in Unfractionated Cell Lysates Using CSox-Based Sensors
      26. Chemoenzymatic Site-Specific Reversible Immobilization and Labeling of Proteins from Crude Cellular Extract Without Prior Purification Using Oxime and Hydrazine Ligation
      27. Synthesis of Hydrogen-Bond Surrogate α-Helices as Inhibitors of Protein-Protein Interactions
      28. Chemical Synthesis of Hydrocarbon-Stapled Peptides for Protein Interaction Research and Therapeutic Targeting
      29. Target Identification by Diazirine Photo-Cross-Linking and Click Chemistry
      30. Targeted Degradation of Proteins by PROTACs
      31. Design, Synthesis, and Application of the Trimethoprim-Based Chemical Tag for Live-Cell Imaging
      32. UbFluor: A Fluorescent Thioester to Monitor HECT E3 Ligase Catalysis
      33. Universal Non-Antibody Detection of Protein Phosphorylation Using pIMAGO
      34. Using a Kinase-Inducible Bimolecular Switch to Control Enzyme Activity in Living Cells
      35. Visualization and Identification of Fatty Acylated Proteins Using Chemical Reporters
      36. Visualization of O-GlcNAc Glycosylation Stoichiometry and Dynamics Using Resolvable Poly(ethylene glycol) Mass Tags
    10. Protein Modifications, Engineering and Applications
    11. Protein Production, Purification, and Analysis
    12. Strategies for Bioactive Small Molecule Discovery
    13. Target Identification for Small Molecules
  8. Cytometry
  9. Gene Expression
  10. Gene Expression
  11. Genetics and Genomics
  12. Imaging and Microscopy
  13. Imaging and Microscopy
  14. Immunology
  15. Immunology
  16. Intermolecular Interactions
  17. Laboratory Organisms and Animal Models
  18. Laboratory Organisms and Animal Models
  19. Microbiology
  20. Microbiology
  21. Molecular Biology
  22. Mouse Biology
  23. Nucleic Acid Chemistry
  24. Pharmacology and Drug Discovery
  25. Pharmacology and Drug Discovery
  26. Protein Production, Purification, and Analysis
  27. Proteomics
  28. RNA
  29. Structural Analysis of Biomolecules
  30. Supporting Laboratory Techniques
  31. Toxicology