Current Protocols in Chemical Biology

Current Protocols in Chemical Biology

Online ISBN: 9780470559277

DOI: 10.1002/9780470559277

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  1. Bioinformatics
  2. Cell Biology
  3. Cell Biology
  4. Cell Culture
  5. Cell Culture
  6. Chemical Biology
  7. Chemical Biology
    1. Carbohydrate Modifications, Engineering and Applications
    2. Chemical Tools for Probing Small Molecule-Macromolecule Interactions
    3. Enzyme Engineering
    4. General Methods in Chemical Biology
      1. Bioorthogonal Profiling of Protein Methylation (BPPM) Using an Azido Analog of S-Adenosyl-L-Methionine
      2. Chemiluminescent Nitrogen Detection (CLND) to Measure Kinetic Aqueous Solubility
      3. Compound Management: Guidelines for Compound Storage, Provision, and Quality Control
      4. Spatiotemporal Control of Gene Expression in Mammalian Cells and in Mice Using the LightOn System
      5. Mass Spectrometry-Based Identification of Protein Kinase Substrates Utilizing Engineered Kinases and Thiophosphate Labeling
      6. Kinase-Catalyzed Biotinylation of Peptides, Proteins, and Lysates
      7. Small-Molecule Library Synthesis on Silicon-Functionalized SynPhase Lanterns
      8. Metallomics Using Inductively Coupled Plasma Mass Spectrometry
      9. Nanostructure-Initiator Mass Spectrometry (NIMS) for the Analysis of Enzyme Activities
      10. On-Bead Two-Color (OBTC) Cell Screen for Direct Identification of Highly Selective Cell Surface Receptor Ligands
      11. One-Pot Multienzyme Synthesis of Lewis x and Sialyl Lewis x Antigens
      12. Profiling Protein Methylation with Cofactor Analog Containing Terminal Alkyne Functionality
      13. In Vivo Imaging and Biochemical Characterization of Protease Function Using Fluorescent Activity-Based Probes
      14. Measurement of Protein Phosphorylation Stoichiometry by SRM-MS
      15. Chemoenzymatic Site-Specific Reversible Immobilization and Labeling of Proteins from Crude Cellular Extract Without Prior Purification Using Oxime and Hydrazine Ligation
      16. Target Identification Using Drug Affinity Responsive Target Stability (DARTS)
      17. Targeted Degradation of Proteins by PROTACs
      18. Design, Synthesis, and Application of the Trimethoprim-Based Chemical Tag for Live-Cell Imaging
      19. Using a Kinase-Inducible Bimolecular Switch to Control Enzyme Activity in Living Cells
    5. High-Content Assay Design
    6. High-Throughput Assay Design
    7. Lipid Modifications, Engineering and Applications
    8. Nucleic Acid Modifications, Engineering and Applications
    9. Protein Modification and Labeling
    10. Protein Modifications, Engineering and Applications
    11. Strategies for Bioactive Small Molecule Discovery
    12. Target Identification for Small Molecules
  8. Cytometry
  9. Gene Expression
  10. Gene Expression
  11. Genetics and Genomics
  12. Imaging and Microscopy
  13. Imaging and Microscopy
  14. Immunology
  15. Immunology
  16. Intermolecular Interactions
  17. Laboratory Organisms and Animal Models
  18. Laboratory Organisms and Animal Models
  19. Microbiology
  20. Microbiology
  21. Molecular Biology
  22. Nucleic Acid Chemistry
  23. Pharmacology and Drug Discovery
  24. Pharmacology and Drug Discovery
  25. Protein Production, Purification, and Analysis
  26. Proteomics
  27. RNA
  28. Structural Analysis of Biomolecules
  29. Supporting Laboratory Techniques