Assay Development: Fundamentals and Practices

Assay Development: Fundamentals and Practices

Author(s): Ge Wu

Published Online: 16 JUN 2010

Print ISBN: 9780470191156

Online ISBN: 9780470583128

DOI: 10.1002/9780470583128

About this Book

Essential principles and practice of assay development

The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.

Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:

  • The basics of assay development, including foundational concepts and applications

  • Commonly used instrumental methods for both biochemical assays and cell-based assays

  • Assay strategies for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.

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