Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition

Copyright © 2010 John Wiley & Sons, Inc.

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Sixth Edition

Author(s): R. Ian Freshney

Published Online: 9 MAR 2011 09:57AM EST

Print ISBN: 9780470528129

Online ISBN: 9780470649367

DOI: 10.1002/9780470649367

About this Book

Product Information

About The Product

This is the sixth edition of the leading text in the basic methodology of cell culture, worldwide. Rigorously revised, it features updates on specialized techniques in stem cell research and tissue engineering; updates on molecular hybridization, somatic cell fusion, hybridomas, and DNA transfer; new sections on vitrification and Organotypic Culture, and new chapters on epithelial, mesenchymal, neurectodermal, and hematopoietic cells; germs cells/stemcells/amniocytes; and non-mammalian/avian cells. It is written for graduate students, research and clinical scientists, and technicians and laboratory managers in cell and molecular biology labs and genetics labs.

PowerPoint slides of the figures as well as other supplementary materials are available at a companion website: www.wiley.com/go/freshney/cellculture

Table of contents

    1. You have free access to this content
      Frontmatter (pages i–xxxi)

    2. You have free access to this content
      Color Plates (pages c1–c28)

    3. Chapter 1

      Introduction (pages 1–10)

    4. Chapter 2

      Biology of Cultured Cells (pages 11–23)

    5. Chapter 3

      Laboratory Design, Layout, and Equipment (pages 25–36)

    6. Chapter 4

      Equipment and Materials (pages 37–56)

    7. Chapter 5

      Aseptic Technique (pages 57–70)

    8. Chapter 6

      Safety, Bioethics, and Validation (pages 71–88)

    9. Chapter 7

      Culture Vessels and Substrates (pages 89–98)

    10. Chapter 8

      Defined Media and Supplements (pages 99–114)

    11. Chapter 9

      Serum-Free Media (pages 115–132)

    12. Chapter 10

      Preparation and Sterilization (pages 133–162)

    13. Chapter 11

      Primary Culture (pages 163–186)

    14. Chapter 12

      Subculture and Cell Lines (pages 187–206)

    15. Chapter 13

      Cloning and Selection (pages 207–225)

    16. Chapter 14

      Cell Separation (pages 227–237)

    17. Chapter 15

      Characterization (pages 239–268)

    18. Chapter 16

      Differentiation (pages 269–278)

    19. Chapter 17

      Transformation and Immortalization (pages 279–297)

    20. Chapter 18

      Contamination (pages 299–315)

    21. Chapter 19

      Cryopreservation (pages 317–334)

    22. Chapter 20

      Quantitation (pages 335–364)

    23. Chapter 21

      Cytotoxicity (pages 365–381)

    24. Chapter 22

      Specialized Cells (pages 383–432)

    25. Chapter 23

      Stem Cells, Germ Cells, and Amniocytes (pages 433–462)

    26. Chapter 24

      Culture of Tumor Cells (pages 463–479)

    27. Chapter 25

      Three-Dimensional Culture (pages 481–495)

    28. Chapter 26

      Scale-Up and Automation (pages 497–515)

    29. Chapter 27

      Specialized Techniques (pages 517–532)

    30. Chapter 28

      Training Programs (pages 533–568)

    31. Chapter 29

      Problem Solving (pages 569–591)

    32. Chapter 30

      In Conclusion (page 593)

    33. You have free access to this content
      Appendix I: Calculations and Preparation of Reagents (pages 595–602)

    34. You have free access to this content
      Appendix II: Sources of Equipment and Materials (pages 603–622)

    35. You have free access to this content
      Appendix III: Suppliers and Other Resources (pages 623–631)

    36. You have free access to this content
      Appendix IV: Glossary (pages 633–638)

    37. You have free access to this content
      Appendix V: Cross-contaminated or Misidentified Cell Lines (pages 639–659)

    38. You have free access to this content
      Appendix VI: General Textbooks and Relevant Journals (pages 661–662)

    39. You have free access to this content
      References (pages 663–715)

    40. You have free access to this content
      Index (pages 717–732)

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