Mass Spectrometry for Microbial Proteomics

Mass Spectrometry for Microbial Proteomics

Editor(s): Haroun N. Shah, Saheer E. Gharbia

Published Online: 15 JUN 2010

Print ISBN: 9780470681992

Online ISBN: 9780470665497

DOI: 10.1002/9780470665497

About this Book

New advances in proteomics, driven largely by developments in mass spectrometry, continue to reveal the complexity and diversity of pathogenic mechanisms among microbes that underpin infectious diseases. Therefore a new era in medical microbiology is demanding a rapid transition from current procedures to high throughput analytical systems for the diagnosis of microbial pathogens.

This book covers the broad microbiological applications of proteomics and mass spectrometry. It is divided into six sections that follow the general progression in which most microbiology laboratories are approaching the subject -Transition, Tools, Preparation, Profiling by Patterns, Target Proteins, and Data Analysis.

Table of contents

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  1. Part I: Microbial Characterisation; the Transition from Conventional Methods to Proteomics

    1. Chapter 1

      Changing Concepts in the Characterisation of Microbes and the Influence of Mass Spectrometry (pages 1–34)

      Haroun N. Shah, Caroline Chilton, Lakshani Rajakaruna, Tom Gaulton, Gillian Hallas, Hristo Atanassov, Ghalia Khoder, Paulina D. Rakowska, Eleonora Cerasoli and Saheer E. Gharbia

  2. Part II: Proteomics Tools and Biomarker Discovery

  3. Part III: Protein Samples: Preparation Techniques

  4. Part IV: Characterisation of Microorganisms by Pattern Matching of Mass Spectral Profiles and Biomarker Approaches Requiring Minimal Sample Preparation

  5. Part V: Targeted Molecules and Analysis of Specific Microorganisms

  6. Part VI: Statistical Analysis of 2D Gels and Analysis of Mass Spectral Data

  7. Part VII: DNA Resequencing by MALDI-TOF-Mass Spectrometry and Its Application to Traditional Microbiological Problems

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