Genomics: Essential Methods

Genomics: Essential Methods

Editor(s): Mike Starkey, Ramnath Elaswarapu

Published Online: 22 NOV 2010 12:55AM EST

Print ISBN: 9780470711576

Online ISBN: 9780470711675

DOI: 10.1002/9780470711675

About this Book

Genomics research has made significant advances in recent years. In this book, a team of internationally-renowned researchers share the most up-to-date information in a field that has in recent years switched emphasis from gene identification to functional genomics and the characterization of genes and gene products. This volume approaches this complex subject with a broad perspective to supply the reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Expansive and current, this book is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice for immediate use at the bench.

Key features:

  • Provides a broad introduction to current practices and techniques for lab-based research in genomics
  • Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches
  • Chapters are written by a leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in genomics
  • Includes troubleshooting guide and reviews of alternative techniques
  • An essential laboratory manual for students and researchers at all levels

Table of contents

    1. You have free access to this content
    2. You have free access to this content
    3. Chapter 1

      High-Resolution Analysis of Genomic Copy Number Changes (pages 1–31)

      Mario Hermsen, Jordy Coffa, Bauke Ylstra, Gerrit Meijer, Hans Morreau, Ronald van Eijk, Jan Oosting and Tom van Wezel

    4. Chapter 3

      Genotyping and LOH Analysis on Archival Tissue using SNP Arrays (pages 49–66)

      Ronald van Eijk, Anneke Middeldorp, Esther H. Lips, Marjo van Puijenbroek, Hans Morreau, Jan Oosting and Tom van Wezel

    5. Chapter 5

      RNA Amplification Strategies: Toward Single-Cell Sensitivity (pages 91–119)

      Natalie Stickle, Norman N. Iscove, Carl Virtanen, Mary Barbara, Carolyn Modi, Toni Di Berardino, Ellen Greenblatt, Ted Brown and Neil Winegarden

    6. Chapter 7

      Gene Expression in Mammalian Cells (pages 155–172)

      Félix Recillas-Targa, Georgina Guerrero, Martín Escamilla-del-Arenal and Héctor Rincón-Arano

    7. Chapter 12

      Gene Therapy Strategies: Constructing an AAV Trojan Horse (pages 283–306)

      M. Ian Phillips, Edilamar M. de Oliveira, Leping Shen, Yao Liang Tang and Keping Qian

    8. You have free access to this content