Practical Methods for Biocatalysis and Biotransformations

Practical Methods for Biocatalysis and Biotransformations

Editor(s): John Whittall, Peter Sutton

Published Online: 15 DEC 2009

Print ISBN: 9780470519271

Online ISBN: 9780470748589

DOI: 10.1002/9780470748589

About this Book

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities.

Practical Methods for Biocatalysis and Biotransformations is a "how-to" guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. Contents include:

  • Introduction to the application of biocatalysts in the pharma industry
  • introduction to molecular biological techniques for biocatalyst development
  • Lipase and protease methods including enzyme supports and dynamic kinetic resolutions
  • Nitrile hydrolases and hydratases
  • Alcohol dehydrogenases and co-factor recycling
  • Epoxide hydrolases
  • Amino acid synthesis
  • Whole cell processes
  • Other biocatalysts, including monoamineoxidases, aminotransterases, and lyases

Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.

Table of contents

    1. You have free access to this content
    2. Chapter 1

      Biotransformations in Small-Molecule Pharmaceutical Development (pages 1–82)

      Joseph P. Adams, Andrew J. Collis, Richard K. Henderson and Peter W. Sutton

    3. Chapter 3

      Kinetic Resolutions Using Biotransformations (pages 117–131)

      Petra Gadler, Kurt Faber, Christopher K. Savile, Romas J. Kazlauskas, Andrew J. Carnell and Gary Breen

    4. Chapter 4

      Dynamic Kinetic Resolution for the Synthesis of Esters, Amides and Acids Using Lipases (pages 133–164)

      Kam Loon Fow, Yongzhong Zhu, Gaik Khuan Chuah, Stephan Jaenicke, S. F. G. M. van Nispen, J. van Buijtenen, J. A. J. M. Vekemans, J. Meuldijk, L. A. Hulshof, Michael Page, John Blacker, Matthew Stirling, Soo-Byung Ko, Mahn-Joo Kim, Jaiwook Park, Stéphane Gastaldi, Gérard Gil, Michèle P. Bertrand, A. H. Kamaruddin, Matthew Truppo, David Pollard, Jeffrey Moore and Paul Devine

    5. Chapter 5

      Enzymatic Selectivity in Synthetic Methods (pages 165–202)

      Xue-Zhong Zhang, Rui-Zhen Hou, Li Xu, Yi-Bing Huang, Miguel Ferrero, Susana Fernández, Vicente Gotor, Alan Taylor, Tek Chand Bhalla, Marloes A. Wijdeven, Piotr Kiełbasiński, Floris P. J. T. Rutjes, Takeshi Sugai, Aya Fujino, Hitomi Yamaguchi, Masaya Ikunaka, Maja Majerić Elenkov, Lixia Tang, Bernhard Hauer and Dick B. Janssen

    6. Chapter 6

      Aldolase Enzymes for Complex Synthesis (pages 203–225)

      William A. Greenberg, Chi-Huey Wong, Marielle Lemaire, Lahssen El Blidi, José A. Castillo, Teodor Parella, Tomoyuki Inoue, Georg A. Sprenger, Jesús Joglar, Pere Clapés, Franck Charmantray, Philippe Dellis, Virgil Hélaine, Soth Samreth and Laurence Hecquet

    7. Chapter 7

      Enzymatic Synthesis of Glycosides and Glucuronides (pages 227–254)

      Adrian Scaffidi, Robert V. Stick, Vladimír Křen, Pavla Bojarová, Wen-Ya Lu, Guo-Qiang Lin, Hui-Lei Yu, Ai-Ming Tong, Jian-He Xu, Cosimo Chiriví, Francesca Sagui, Sergio Riva, Olha Khymenets, Pere Clapés, Teodor Parella, María-Isabel Covas, Rafael de la Torre, Jesús Joglar, Matthias Kittelmann, Lukas Oberer, Reiner Aichholz and Oreste Ghisalba

    8. Chapter 8

      Synthesis of Cyanohydrins Using Hydroxynitrile Lyases (pages 255–272)

      Manuela Avi, Herfried Griengl, Chris Roberge, Fred Fleitz, Paul Devine, Jan von Langermann, Annett Mell, Eckhard Paetzold, Udo Kragl and Yasuhisa Asano

    9. Chapter 9

      Synthesis of Chiral sec-Alcohols by Ketone Reduction (pages 273–294)

      David Pollard, Matthew Truppo, Jeffrey Moore, Birgit Kosjek, David Tellers, Jeffrey Moore, Ioulia Smonou, Dimitris Kalaitzakis, Musa M. Musa, Karla I. Ziegelmann-Fjeld, Claire Vieille, J. Gregory Zeikus, Robert S. Phillipsa, Matthew Truppo, Krista Morley, David Pollard, Paul Devine, Tadashi Ema, Nobuyasu Okita, Sayaka Ide and Takashi Sakai

    10. Chapter 10

      Reduction of Functional Groups (pages 295–317)

      Andrew S. Lamm, Arshdeep Khare, John P. N. Rosazza, Andreas Taglieber, Frank Schulz, Frank Hollmann, Monika Rusek, Manfred T. Reetz, Thierry Gefflaut, Emmanuelle Sagot, Jean Bolte, Hisaaki Mihara, Nobuyoshi Esaki, Philip Conway, Francesca Paradisi and Paul Engel

    11. Chapter 11

      Enzymatic Oxidation Chemistry (pages 319–350)

      Andrew J. Ellis, Renate Reiss, Timothy J. Snape, Nicholas J. Turner, Fabio Pezzotti, Helene Therisod, Michel Therisod, Bjoern-Arne Kaup, Umberto Piantini, Matthias Wüst, Jens Schrader, Cinzia Chiappe, Stefano Colonna, Nicoletta Gaggero, Sara Pellegrino, Francesca Zambianchi, Anett Kirschner, Uwe T. Bornscheuer, Gideon Grogan, Cheryl Hill, Shaozhao Wang, Jianzhong Yang and Peter C. K. Lau

    12. Chapter 12

      Whole-Cell Oxidations and Dehalogenations (pages 351–396)

      Arshdeep Khare, Andrew S. Lamm, John P.N. Rosazza, Sheng Yuan, Yi-Jun Dai, Matthias Kittelmann, Maria Serrano Correia, Anton Kuhn, Serge Parel, Jürgen Kühnöl, Reiner Aichholz, Monique Ponelle, Oreste Ghisalba, Koichi Mitsukura, Yoshinori Kondo, Toyokazu Yoshida, Toru Nagasawa, Renata P. Limberger, Cleber V. Ursini, Paulo J. S. Moran, J. Augusto R. Rodrigues, Marco Antonio Mirata, Jens Schrader, Louise C. Nolan, Kevin E. O'Connor, Katja Buehler, Andreas Schmid, Takamitsu Utsukihara and C. Akira Horiuchi

    13. You have free access to this content