Antigen Retrieval Immunohistochemistry Based Research and Diagnostics

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics

Editor(s): Shan-Rong Shi, Clive R. Taylor

Published Online: 4 AUG 2010

Print ISBN: 9780470624524

Online ISBN: 9780470875612

DOI: 10.1002/9780470875612

About this Book

The most complete, up-to-date reference on antigen retrieval and immunohistochemistry

An antigen is a substance that prompts the generation of antibodies and can cause an immune response. The antigen retrieval (AR) technique is in wide use across the globe, and is a critical technique used in medical diagnosis of disease, particularly clinical targeted cancer treatment.

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics discusses several scientific approaches to the standardization of quantifiable immunohistochemistry (IHC). Based on the development and application of AR by the editors, this volume summarizes recent achievements in AR-IHC and analyzes numerous cutting-edge issues for future research projects.

Featuring contributions from a worldwide group of leading experts and research scientists in the field, this important work:

  • Summarizes the key problems in the four fields of antigen retrieval
  • Discusses the advances of AR techniques and their applications
  • Provides practical methods and protocols in AR-IHC, such as extraction of nucleic acids and proteins for molecular analysis, cell/tissue sample preparation, and standardization and development of various techniques to meet the future needs of¿clinical and research molecular analysis
  • Encourages further research in AR and IHC, particularly how AR methods might be employed for improved test performance and the development of greater reliability and reproducibility of IHC
  • Includes an appendix of related laboratory protocols

Antigen Retrieval Immunohistochemistry Based Research and Diagnostics is intended for clinical pathologists, molecular cell biologists, basic research scientists, technicians, and graduate students who undertake tissue/cell morphologic and molecular analysis and wish to use and extend the power of immunohistochemistry. It is also pertinent for most biotechnology companies majoring in development of IHC products.

Wiley Series in Biomedical Engineering and Multi-Disciplinary Integrated Systems / Kai Chang, Series Editor

Table of contents

    1. You have free access to this content
    1. You have free access to this content
  1. Part I: Recent Advances in Antigen Retrieval Techniques and Its Application

  2. Part II: Standardization of Immunohistochemistry

  3. Part III: Tissue/Cell Sample Preparation

  4. Part IV: Molecular Mechanism of Antigen Retrieval Technique

    1. Chapter 18

      Commentary: Future Directions (pages 323–331)

      Timothy J. O'leary, Carol B. Fowler, David L. Evers, Robert E. Cunningham and Jeffrey T. Mason

  5. Part V: Proteomic Analysis of Protein Extracted from Tissue/Cells

    1. You have free access to this content
    1. You have free access to this content

SEARCH