Human Stem Cell Technology and Biology: A Research Guide and Laboratory Manual

Copyright © 2011 Wiley-Blackwell

Human Stem Cell Technology and Biology: A Research Guide and Laboratory Manual

Editor(s): Gary S. Stein, Maria Borowski, Mai X. Luong, Meng-Jiao Shi, Kelly P. Smith, Priscilla Vazquez

Published Online: 29 NOV 2010

Print ISBN: 9780470595459

Online ISBN: 9780470889909

DOI: 10.1002/9780470889909

About this Book

Product Information

About The Product

Human Stem Cell Technology & Biology: A Research Guide and Laboratory Manual integrates readily accessible text, electronic and video components with the aim of effectively communicating the critical information needed to understand and culture human embryonic stem cells.

Key Features:

  • An authoritative, comprehensive, multimedia training manual for stem cell researchers
  • Easy to follow step-by-step laboratory protocols and instructional videos provide a valuable resource
  • A must-have for developing laboratory course curriculums, training courses, and workshops in stem cell biology
  • Perspectives written by the world leaders in the field
  • Introductory chapters will provide background information

The volume will be a valuable reference resource for both experienced investigators pursuing stem cell and induced pluripotent stem cell research as well as those new to this field.

Table of contents

    1. You have free access to this content
      Frontmatter (pages i–xvii)

  2. Section I: Introduction

    1. Chapter 1

      Introduction to Pluripotent Stem Cells: Biology and Applications (pages 1–7)

      Maria Borowski and Gary S. Stein

    2. Chapter 2

      Researching and Obtaining Established Stem Cell Lines (pages 9–16)

      Mai X. Luong, Kelly P. Smith and Gary S. Stein

  3. Section II: Laboratory Guide for Human Stem Cell Culture: Pluripotent Stem Cell Culture

    1. Chapter 3

      Basics of Cell Culture (pages 17–31)

      Alicia Allaire, Mai X. Luong and Kelly P. Smith

    2. Chapter 4

      The Stem Cell Laboratory (pages 33–40)

      Alicia Allaire, Mai X. Luong and Kelly P. Smith

    3. Chapter 5

      Reagent Preparation (pages 41–58)

      Alicia Allaire, Mai X. Luong and Kelly P. Smith

    4. Chapter 6

      Preparation of Mouse Embryonic Fibroblasts for Culture of Human Embryonic Stem Cells (pages 59–85)

      Meng-Jiao Shi, Maria Borowski and Kimberly Stencel

    5. Chapter 7

      Thawing and Seeding of Frozen Mouse Embryonic Fibroblasts (pages 87–93)

      Meng-Jiao Shi, Maria Borowski and Kimberly Stencel

    6. Chapter 8

      Thawing, Seeding, and Changing the Medium of Human Embryonic Stem Cells (pages 95–104)

      Meng-Jiao Shi, Kimberly Stencel and Maria Borowski

    7. Chapter 9

      Passaging of Human Embryonic Stem Cells on Inactivated Mouse Embryonic Fibroblast Plates (pages 105–117)

      Meng-Jiao Shi, Kimberly Stencel and Maria Borowski

    8. Chapter 10

      Harvesting Human Embryonic Stem Cells for Cryopreservation (pages 119–127)

      Meng-Jiao Shi, Kimberly Stencel and Maria Borowski

    9. Chapter 11

      Human Embryonic Stem Cell Culture on BD Matrigel™ with mTeSR®1 Medium (pages 129–137)

      Meng-Jiao Shi, Kimberly Stencel and Maria Borowski

  4. Section III: Laboratory Guide for Human Stem Cell Culture: Characterization of Pluripotent Stem Cells

    1. Chapter 12

      Defining Pluripotency (pages 139–148)

      Kelly P. Smith and Mai X. Luong

    2. Chapter 13

      Characterization of Human Embryonic Stem Cells by Immunofluorescence Microscopy (pages 149–157)

      Shirwin M. Pockwinse and Prachi N. Ghule

    3. Chapter 14

      Preparation of Human Embryonic Stem Cell Samples for Flow Cytometry (pages 159–165)

      Meng-Jiao Shi, Kimberly Stencel and Maria Borowski

    4. Chapter 15

      Characterization of Human Embryonic Stem Cell Gene Expression Using Reverse Transcription Polymerase Chain Reaction (pages 167–189)

      Alicia Allaire, Kelly P. Smith and Mai X. Luong

    5. Chapter 16

      Determining Pluripotency of Human Embryonic Stem Cells: Embryoid Body Formation (pages 191–197)

      Shirwin M. Pockwinse and Prachi N. Ghule

    6. Chapter 17

      Characterization of Human Embryonic Stem Cells by Cytogenetics: Karyotyping and Fluorescence In Situ Hybridization (pages 199–208)

      Shirwin M. Pockwinse, Prachi N. Ghule and Anne Higgins

    7. Chapter 18

      High-Resolution Chromatin Immunoprecipitation Assay (pages 211–216)

      Beatriz Pérez-Cadahía, Bojan Drobic and James R. Davie

    8. Chapter 19

      Assaying Pluripotency Via Teratoma Formation (pages 217–233)

      M. William Lensch and Tan A. Ince

  5. Section IV: Perspectives in Human Stem Cell Technologies

    1. Chapter 20

      Genomic Analysis of Pluripotent Stem Cells (pages 235–243)

      David Lapointe

    2. Chapter 21

      Proteomic Analysis of Human Pluripotent Cells (pages 245–256)

      Andy T. Y. Lau, Yan-Ming Xu and Jen-Fu Chiu

    3. Chapter 22

      Bioinformatics Strategies for Understanding Gene Expression in Human Pluripotent Cells (pages 257–272)

      Gustavo Glusman, Bruz Marzolf, Kai Wang, Ji-Hoon Cho, Burak Kutlu and Qiang Tian

    4. Chapter 23

      Epigenetic Analysis of Pluripotent Cells (pages 273–288)

      Mojgan Rastegar, Geneviève P. Delcuve and James R. Davie

    5. Chapter 24

      Differentiation of Pluripotent Stem Cells: An Overview (pages 289–295)

      Jeremy Micah Crook

    6. Chapter 25

      Cellular Reprogramming: Current Technology, Perspectives, and Generation of Induced Pluripotent Cells (pages 297–310)

      Tanja Dominko

  6. Section V: Applications of Human Embryonic Stem Cells

    1. Chapter 26

      Human Pluripotent Cells: The Biology of Pluripotency (pages 311–325)

      Li-Fang Chu and Thomas P. Zwaka

    2. Chapter 27

      Human Pluripotent Cells for Regenerative Medicine: Potential Applications for Regenerative Medicine (pages 327–337)

      Christopher C. Ford and Darrell N. Kotton

    3. Chapter 28

      Therapeutic Applications of Human Embryonic Stem Cells (pages 339–352)

      Shi-Jiang Lu, Irina Klimanskaya, Edmund Mickunas and Robert Lanza

    4. Chapter 29

      Human Stem Cells for Drug Screening, Toxicity, Specificity, and Off-Target Effects (pages 353–365)

      Arnaud Lacoste and Mark Burcin

    5. Chapter 30

      Interfacing Stem Cells with Gene Therapy (pages 367–375)

      Christian Mueller and Terence R. Flotte

    6. Chapter 31

      Tissue Engineering for Stem Cell Mediated Regenerative Medicine (pages 377–399)

      Janet Zoldan, Thomas P. Kraehenbuehl, Abigail K. R. Lytton-Jean, Robert S. Langer and Daniel G. Anderson

    1. You have free access to this content
      Glossary (pages 401–411)

    1. You have free access to this content
      Index (pages 413–419)

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