Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Editor(s): Jan-Christer Janson

Published Online: 11 MAR 2011 11:48AM EST

Print ISBN: 9780471746614

Online ISBN: 9780470939932

DOI: 10.1002/9780470939932

About this Book

The authoritative guide on protein purification-now completely updated and revised

Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch.

Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes:

  • High-resolution reversed-phase liquid chromatography

  • Electrophoresis in gels

  • Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients

  • Affinity ligands from chemical and biological combinatorial libraries

  • Membrane separations

  • Refolding of inclusion body proteins from E. coli

  • Purification of PEGylated proteins

  • High throughput screening techniques in protein purification

  • The history of protein chromatography

Table of contents

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    1. You have free access to this content
  1. Part I: Introduction

  2. Part II: Chromatography

    1. Chapter 8

      Covalent Chromatography (pages 203–219)

      Francisco Batista-Viera, Lars Rydén and Jan Carlsson

    2. Chapter 9

      Affinity Chromatography (pages 221–258)

      Francisco Batista-Viera, Jan-Christer Janson and Jan Carlsson

  3. Part III: Other Separation Methods and Related Techniques

    1. Chapter 12

      Membrane Separations (pages 279–317)

      Joachim K. Walter, Zuwei Jin, Maik W. Jornitz and Uwe Gottschalk

  4. Part IV: Electrophoresis

  5. Part V: Separation Method Optimization

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