Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Protein Purification: Principles, High Resolution Methods, and Applications, Third Edition

Editor(s): Jan-Christer Janson

Published Online: 11 MAR 2011 11:48AM EST

Print ISBN: 9780471746614

Online ISBN: 9780470939932

DOI: 10.1002/9780470939932

About this Book

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About The Product

This thoroughly revised and updated third edition provides an overview of the principles and methodology of the most frequently used biochemical separation techniques for proteins used today. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:

  • Gel filtration and ion exchange chromatography
  • Affinity and covalent chromatography 
  • Reversed phase and hydrophobic interaction chromatography 
  • Ligands from combinatorial libraries 
  • Electrophoresis in gels 
  • Immunoelectrophoresis and capillary electrophoresis 
  • Two-dimensional electrophoresis in proteomics
  • Protein elution and blotting techniques
  • Large-scale electrophoresis processes

Table of contents

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    1. You have free access to this content
  1. Part I: Introduction

  2. Part II: Chromatography

    1. Chapter 8

      Covalent Chromatography (pages 203–219)

      Francisco Batista-Viera, Lars Rydén and Jan Carlsson

    2. Chapter 9

      Affinity Chromatography (pages 221–258)

      Francisco Batista-Viera, Jan-Christer Janson and Jan Carlsson

  3. Part III: Other Separation Methods and Related Techniques

    1. Chapter 12

      Membrane Separations (pages 279–317)

      Joachim K. Walter, Zuwei Jin, Maik W. Jornitz and Uwe Gottschalk

  4. Part IV: Electrophoresis

  5. Part V: Separation Method Optimization

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