Current Protocols in Microbiology

Current Protocols in Microbiology

Online ISBN: 9780471729259

DOI: 10.1002/9780471729259

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Current Protocols in Microbiology Supplements

2014:   Supplement 34    Supplement 33    Supplement 32    
2013:   Supplement 31    Supplement 30    Supplement 29    Supplement 28    
2012:   Supplement 27    Supplement 26    Supplement 25    Supplement 24    
2011:   Supplement 23    Supplement 22    Supplement 21    Supplement 20
2010:   Supplement 19    Supplement 18    Supplement 17    Supplement 16
2009:   Supplement 15    Supplement 14    Supplement 13    Supplement 12
2008:   Supplement 11    Supplement 10    Supplement 9    Supplement 8    
2007:   Supplement 7    Supplement 6    Supplement 5    Supplement 4    
2006:   Supplement 3    Supplement 2    Supplement 1    Core publication




  Supplement 34, August 2014

Unit 1F.3 Quantitative Proteomic Analysis of the Cell Envelopes and Native Membrane Vesicles Derived from Gram-Negative Bacteria
         Abstract | Full Text:  HTML   PDF

Unit 4A.3 Isolation of Cell Envelopes and Naturally Released Membrane Vesicles of Neisseria gonorrhoeae
         Abstract | Full Text:  HTML   PDF

Unit 12C.5 Molecular Typing of Borrelia burgdorferi
         Abstract | Full Text:  HTML   PDF

Unit 14B.3 Using Organotypic (Raft) Epithelial Tissue Cultures for the Biosynthesis and Isolation of Infectious Human Papillomaviruses
         Abstract | Full Text:  HTML   PDF

Unit 15I.1 Avian Bornaviruses: Diagnosis, Isolation, and Genotyping
         Abstract | Full Text:  HTML   PDF

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  Supplement 33, May 2014

Unit 1G.1 Biological Sand Filters: Low-Cost Bioremediation Technique for Production of Clean Drinking Water
         Abstract | Full Text:  HTML   PDF

Unit 1G.2 Bioremediation of Turbid SurfaceWater Using Seed Extract from the Moringa oleifera Lam. (Drumstick) Tree
         Abstract | Full Text:  HTML   PDF

Unit 9B.5 Isolation of Listeria monocytogenes from Food and Water: Official and Experimental Protocols
         Abstract | Full Text:  HTML   PDF

Unit 14B.2 Analyzing the Human Papillomavirus (HPV) Life Cycle in Primary Keratinocytes with a Quantitative Colony-Forming Assay
         Abstract | Full Text:  HTML   PDF

Unit 15K.2 Murine Norovirus: Propagation, Quantification, and Genetic Manipulation
         Abstract | Full Text:  HTML   PDF

Unit 18.4 Western Blot Assay for Quantitative and Qualitative Antigen Detection in Vaccine Development
         Abstract | Full Text:  HTML   PDF

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  Supplement 32, February 2014

Unit 1E.7 Gene Identification in Prokaryotic Genomes, Phages, Metagenomes, and EST Sequences with GeneMarkS Suite
         Abstract | Full Text:  HTML   PDF

Unit 6F.1 Laboratory Culture and Maintenance of Stenotrophomonas maltophilia
         Abstract | Full Text:  HTML   PDF

Unit 9C.3 Genetic Manipulation of Staphylococcus aureus
         Abstract | Full Text:  HTML   PDF

Unit 12E.5 Leptospirosis Serodiagnosis by the Microscopic Agglutination Test
         Abstract | Full Text:  HTML   PDF

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  Supplement 31, November 2013

Unit 9B.2 Listeria monocytogenes Cultivation and Laboratory Maintenance
         Abstract | Full Text:  HTML   PDF

Unit 9B.3 A Mouse Model of Foodborne Listeria monocytogenes Infection
         Abstract | Full Text:  HTML   PDF

Unit 15D.3 Propagation, Quantification, Detection, and Storage of West Nile Virus
         Abstract | Full Text:  HTML   PDF

Unit 15K.1 Human Norovirus Detection and Production, Quantification, and Storage of Virus-Like Particles
         Abstract | Full Text:  HTML   PDF

Unit 18.3 DNA Immunization
         Abstract | Full Text:  HTML   PDF

Appendix 3N Care and Handling of Laboratory Mice
         Abstract | Full Text:  HTML   PDF

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  Supplement 30, October 2013

Unit 9A.3 Therapeutic Approaches for Clostridium difficile Infections
         Abstract | Full Text:  HTML   PDF

Unit 9D.2 Laboratory Growth and Maintenance of Streptococcus pyogenes (The Group A Streptococcus, GAS)
         Abstract | Full Text:  HTML   PDF

Unit 9D.3 Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)
         Abstract | Full Text:  HTML   PDF

Unit 15H.3 RNA Structure Analysis of Viruses Using SHAPE
         Abstract | Full Text:  HTML   PDF

Unit 18.2 Newcastle Disease Virus-Like Particles: Preparation, Purification, Quantification, and Incorporation of Foreign Glycoproteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 29, May 2013

Unit 14D.5 Gene Transfer to the CNS Using Recombinant Adeno-Associated Virus
         Abstract | Full Text:  HTML   PDF

Unit 14D.6 Gene Transfer in the Liver Using Recombinant Adeno-Associated Virus
         Abstract | Full Text:  HTML   PDF

Unit 15G.1 Influenza: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 15G.4 Insertion of a GFP Reporter Gene in Influenza Virus
         Abstract | Full Text:  HTML   PDF

Unit 15H.1 Poliovirus: Generation, Quantification, Propagation, Purification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 15H.2 Poliovirus: Generation and Characterization of Mutants
         Abstract | Full Text:  HTML   PDF

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  Supplement 28, February 2013

Unit 1E.6 Analyzing Arthropods for the Presence of Bacteria
         Abstract | Full Text:  HTML   PDF

Unit 9C.1 Growth and Laboratory Maintenance of Staphylococcus aureus
         Abstract | Full Text:  HTML   PDF

Unit 9C.2 Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA)
         Abstract | Full Text:  HTML   PDF

Unit 14D.3 Gene Transfer in Skeletal and Cardiac Muscle Using Recombinant Adeno-Associated Virus
         Abstract | Full Text:  HTML   PDF

Unit 14D.4 Retinal Gene Delivery by rAAV and DNA Electroporation
         Abstract | Full Text:  HTML   PDF

Unit 15J.1 Human Immunodeficiency Viruses: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

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  Supplement 27, November 2012

Unit 1E.5 Using QIIME to Analyze 16S rRNA Gene Sequences from Microbial Communities
         Abstract | Full Text:  HTML   PDF

Unit 2C.5 Use of Image-Based Flow Cytometry in Bacterial Viability Analysis Using Fluorescent Probes
         Abstract | Full Text:  HTML   PDF

Unit 3D.4 Isolation and Cultivation of Agrobacterium Species from Natural Sources
         Abstract | Full Text:  HTML   PDF

Unit 5A.4 Growth and Maintenance of Escherichia coli Laboratory Strains
         Abstract | Full Text:  HTML   PDF

Unit 11A.2 Sphingolipid Trafficking and Purification in Chlamydia trachomatis–Infected Cells
         Abstract | Full Text:  HTML   PDF

Unit 15D.2 Dengue Virus: Isolation, Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

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  Supplement 26, August 2012

Unit 1F.2 2-D Gel-Based Proteomic Approaches to Antibiotic Drug Discovery
         Abstract | Full Text:  HTML   PDF

Unit 6A.5 Detection, Isolation, and Identification of Vibrio cholerae from the Environment
         Abstract | Full Text:  HTML   PDF

Unit 7B.1 Isolation and Classification of Bdellovibrio and Like Organisms
         Abstract | Full Text:  HTML   PDF

Unit 14D.1 Production and Discovery of Novel Recombinant Adeno-Associated Viral Vectors
         Abstract | Full Text:  HTML   PDF

Unit 14D.2 Gene Transfer in the Lung Using Recombinant Adeno-Associated Virus
         Abstract | Full Text:  HTML   PDF

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  Supplement 25, May 2012

Unit 2B.2 Scanning Electron Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 3D.2 Genetic Manipulation of Agrobacterium
         Abstract | Full Text:  HTML   PDF

Unit 3D.3 Phenotypic Analyses of Agrobacterium
         Abstract | Full Text:  HTML   PDF

Unit 6E.1 Growth and Laboratory Maintenance of Pseudomonas aeruginosa
         Abstract | Full Text:  HTML   PDF

Unit 15C.5 Separation and Isolation of BTV dsRNA Segments and Viral Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 24, February 2012

Unit 1F.1 Identification of Novel DNA-Binding Proteins Using DNA-Affinity Chromatography/Pull Down
         Abstract | Full Text:  HTML   PDF

Unit 3D.1 Laboratory Maintenance of Agrobacterium
         Abstract | Full Text:  HTML   PDF

Unit 8B.1 Laboratory Maintenance of Helicobacter Species
         Abstract | Full Text:  HTML   PDF

Unit 14B.1 Cell Culture Assay for Transient Replication of Human and Animal Papillomaviruses
         Abstract | Full Text:  HTML   PDF

Unit 14E.4 Human Cytomegalovirus: Bacterial Artificial Chromosome (BAC) Cloning and Genetic Manipulation
         Abstract | Full Text:  HTML   PDF

Unit 15C.4 Bluetongue Virus (BTV): Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

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  Supplement 23, November 2011

Unit 1A.3 Safe Use of Hazardous Chemicals
         Abstract | Full Text:  HTML   PDF

Unit 1C.1 Methods for Analysis of Bacterial Autoinducer-2 Production
         Abstract | Full Text:  HTML   PDF

Unit 4A.2 Genetic Manipulation of Neisseria gonorrhoeae
         Abstract | Full Text:  HTML   PDF

Unit 5B.3 Extraction, Purification, and Identification of Yersiniabactin, the Siderophore of Yersinia pestis
         Abstract | Full Text:  HTML   PDF

Unit 6A.3 Vibriocidal Assays to Determine the Antibody Titer of Patient Sera Samples
         Abstract | Full Text:  HTML   PDF

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  Supplement 22, August 2011

Unit 1B.1 Growing and Analyzing Static Biofilms
         Abstract | Full Text:  HTML   PDF

Unit 6A.2 Genetic Screens and Biochemical Assays to Characterize Vibrio cholerae O1 Biotypes: Classical and El Tor
         Abstract | Full Text:  HTML   PDF

Unit 12D.1 Isolation and Maintenance of Brachyspira Species
         Abstract | Full Text:  HTML   PDF

Unit 17.6 Laboratory Detection of Vancomycin Nonsusceptible Staphylococcus aureus
         Abstract | Full Text:  HTML   PDF

Unit 17.7 Screening of Hepatitis C Virus Inhibitors Using Genotype 1a HCV Replicon Cell Lines
         Abstract | Full Text:  HTML   PDF

Appendix 3L Generation of Transformation Competent E. coli
         Abstract | Full Text:  HTML   PDF

Appendix 3M SDS-Polyacrylamide gel Electrophoresis (SDS-PAGE) of Proteins
         Abstract | Full Text:  HTML   PDF

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  Supplement 21, May 2011

Unit 1B.2 Growing and Analyzing Biofilms in Flow Chambers
         Abstract | Full Text:  HTML   PDF

Unit 2C.3 Whole-Body Imaging of Infection Using Fluorescence
         Abstract | Full Text:  HTML   PDF

Unit 2C.4 Whole-Body Imaging of Infection Using Bioluminescence
         Abstract | Full Text:  HTML   PDF

Unit 14A.2 Production of Myxoma Virus Gateway Entry and Expression Libraries and Validation of Viral Protein Expression
         Abstract | Full Text:  HTML   PDF

Unit 15E.1 Coronaviruses: Propagation, Quantification, Storage, and Construction of Recombinant Mouse Hepatitis Virus
         Abstract | Full Text:  HTML   PDF

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  Supplement 20, February 2011

Unit 1E.4 Preparing DNA Libraries for Multiplexed Paired-End Deep Sequencing for Illumina GA Sequencers
         Abstract | Full Text:  HTML   PDF

Unit 9A.2 Genetic Manipulation of Clostridium difficile
         Abstract | Full Text:  HTML   PDF

Unit 10A.6 Acid-Fast Staining and Petroff-Hausser Chamber Counting of Mycobacterial Cells in Liquid Suspension
         Abstract | Full Text:  HTML   PDF

Unit 12C.4 Genetic Transformation of Borrelia burgdoferi
         Abstract | Full Text:  HTML   PDF

Unit 12E.3 Rat Model of Chronic Leptospirosis
         Abstract | Full Text:  HTML   PDF

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  Supplement 19, November 2010

Unit 1D.2 Using Host 28S Ribosomal RNA as a Housekeeping Gene for Quantitative Real-Time Reverse Transcription-PCR (qRT-PCR) in Virus-Infected Animal Cells
         Abstract | Full Text:  HTML   PDF

Unit 1E.2 Simultaneous Isolation of Ixodidae and Bacterial (Borrelia spp.) Genomic DNA
         Abstract | Full Text:  HTML   PDF

Unit 1E.3 Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms
         Abstract | Full Text:  HTML   PDF

Unit 10E.2 Laboratory Maintenance of Streptomyces argillaceus and Streptomyces griseus
         Abstract | Full Text:  HTML   PDF

Unit 10E.3 Genetic Manipulation of Streptomyces Species
         Abstract | Full Text:  HTML   PDF

Unit 10E.4 Isolation of Streptomyces Species from Soil
         Abstract | Full Text:  HTML   PDF

Unit 15D.1 Hepatitis C Virus: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 16J.1 A High-Throughput Approach for Studying Virus Replication in Yeast
         Abstract | Full Text:  HTML   PDF

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  Supplement 18, August 2010

Unit 6D.1 Laboratory Growth and Maintenance of Haemophilus influenzae
         Abstract | Full Text:  HTML   PDF

Unit 10D.1 Laboratory Maintenance of Mycobacterium abscessus
         Abstract | Full Text:  HTML   PDF

Unit 10D.2 Genetic Manipulation of Mycobacterium abscessus
         Abstract | Full Text:  HTML   PDF

Unit 10E.1 Laboratory Maintenance of Streptomyces Species
         Abstract | Full Text:  HTML   PDF

Unit 14E.3 Human Cytomegalovirus: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 17.5 Cell-Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening
         Abstract | Full Text:  HTML   PDF

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  Supplement 17, May 2010

Unit 6A.1 Growth and Laboratory Maintenance of Vibrio cholerae
         Abstract | Full Text:  HTML   PDF

Unit 14A.1 Myxoma Virus: Propagation, Purification, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 15F.1 Human Parainfluenza Virus Type 3 (HPIV-3): Construction and Rescue of an Infectious, Recombinant Virus Expressing the Enhanced Green Fluorescent Protein (EGFP)
         Abstract | Full Text:  HTML   PDF

Appendix 2C Where to Find Recipes for Media
         Abstract | Full Text:  HTML   PDF

Appendix 4H Growth and Maintenance of Baby Hamster Kidney (BHK) Cells
         Abstract | Full Text:  HTML   PDF

Appendix 4I Growth and Maintenance of Chick Embryo Fibroblasts (CEK)
         Abstract | Full Text:  HTML   PDF

Appendix 4J Growth and Maintenance of Mosquito Cell Lines
         Abstract | Full Text:  HTML   PDF

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  Supplement 16, February 2010

Unit 1G.2 Bioremediation of Turbid Surface Water Using Seed Extract from Moringa oleifera Lam. (Drumstick) Tree
         Abstract | Full Text:  HTML   PDF

Unit 12C.2 Methods of Identifying Membrane Proteins in Spirochetes
         Abstract | Full Text:  HTML   PDF

Unit 15B.1 Sindbis Virus: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 17.1 Characterization of the In Vitro Activity of Novel Lipoglycopeptide Antibiotics
         Abstract | Full Text:  HTML   PDF

Unit 17.4 Identification of Small-Molecule Scaffolds for P450 Inhibitors
         Abstract | Full Text:  HTML   PDF

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  Supplement 15, November 2009

Unit 1D.1 Example of Use of TaqMan Real-Time RT-PCR to Analyze Gene Transcript Levels: Haemophilus influenzae
         Abstract | Full Text:  HTML   PDF

Unit 4B.1 Laboratory Maintenance of Bordetella pertussis
         Abstract | Full Text:  HTML   PDF

Unit 6C.1 Laboratory Maintenance of Coxiella burnetii
         Abstract | Full Text:  HTML   PDF

Unit 15C.3 Culturing, Storage, and Quantification of Rotaviruses
         Abstract | Full Text:  HTML   PDF

Appendix 3C Differential Staining of Bacteria: Gram Stain
         Abstract | Full Text:  HTML   PDF

Appendix 3E Preliminary Staining of Bacteria: Simple Stains
         Abstract | Full Text:  HTML   PDF

Appendix 3F Preliminary Staining of Bacteria: Negative Stain
         Abstract | Full Text:  HTML   PDF

Appendix 3G Differential Staining of Bacteria: Flagella Stain
         Abstract | Full Text:  HTML   PDF

Appendix 3H Differential Staining of Bacteria: Acid Fast Stain
         Abstract | Full Text:  HTML   PDF

Appendix 3I Differential Staining of Bacteria: Capsule Stain
         Abstract | Full Text:  HTML   PDF

Appendix 3J Differential Staining of Bacteria: Endospore Stain
         Abstract | Full Text:  HTML   PDF

Appendix 3K Fluorescent Staining of Bacteria: Viability and Antibody Labeling
         Abstract | Full Text:  HTML   PDF

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  Supplement 14, August 2009

Unit 5A.3 Genotyping Escherichia coli O157:H7 for Its Ability to Cause Disease in Humans
         Abstract | Full Text:  HTML   PDF

Unit 10C.1 Laboratory Maintenance of Mycobacterium smegmatis
         Abstract | Full Text:  HTML   PDF

Unit 15C.1 Mammalian Reoviruses: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 15C.2 Avian Reoviruses: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 16A.1 Growth Conditions for Plant Virus–Host Studies
         Abstract | Full Text:  HTML   PDF

Unit 16A.2 Propagation of Major Plant-Virus Hosts
         Abstract | Full Text:  HTML   PDF

Appendix 4G Growth and Maintenance of Quail Fibrosarcoma QM5 Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 13, May 2009

Unit 1A.1 Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms
         Abstract | Full Text:  HTML   PDF

Unit 9D.1 Adult Zebrafish Model of Streptococcal Infection
         Abstract | Full Text:  HTML   PDF

Unit 15G.2 The Ferret Model for Influenza
         Abstract | Full Text:  HTML   PDF

Unit 15G.3 The Mouse Model for Influenza
         Abstract | Full Text:  HTML   PDF

Unit 16B.6 Mechanical Inoculation of Plant Viruses
         Abstract | Full Text:  HTML   PDF

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  Supplement 12, February 2009

Unit 9A.1 Laboratory Maintenance of Clostridium difficile
         Abstract | Full Text:  HTML   PDF

Unit 11B.1 Cultivation and Laboratory Maintenance of Chlamydia pneumoniae
         Abstract | Full Text:  HTML   PDF

Unit 16B.4 Fungal Transmission of Plant Viruses
         Abstract | Full Text:  HTML   PDF

Unit 16B.5 Testing of Transmission of Tobraviruses by Nematodes
         Abstract | Full Text:  HTML   PDF

Unit 17.2 Structure-Based Approaches to Antibiotic Drug Discovery
         Abstract | Full Text:  HTML   PDF

Unit 17.3 Novel Approaches to Bacterial Infection Therapy by Interfering with Cell-to-Cell Signaling
         Abstract | Full Text:  HTML   PDF

Appendix 4F Anaerobic Cell Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 11, November 2008

Unit 3A.5 Laboratory Maintenance of Rickettsia rickettsii
         Abstract | Full Text:  HTML   PDF

Unit 5B.1 Laboratory Maintenance and Characterization of Yersinia pestis
         Abstract | Full Text:  HTML   PDF

Unit 5B.2 Isolation and Confirmation of Yersinia pestis Mutants Exempt from Select Agent Regulations
         Abstract | Full Text:  HTML   PDF

Unit 6B.1 Laboratory Maintenance of Moraxella catarrhalis
         Abstract | Full Text:  HTML   PDF

Unit 8A.3 Experimental Chick Colonization by Campylobacter jejuni
         Abstract | Full Text:  HTML   PDF

Appendix 2C Where to Find Recipes for Media
         Full Text:  HTML   PDF

Appendix 4D Aseptic Technique
         Abstract | Full Text:  HTML   PDF

Appendix 4E Growth and Maintenance of Vero Cell Lines
         Abstract | Full Text:  HTML   PDF

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  Supplement 10, August 2008

Unit 1B.4 Preparing for Biofilm Studies in the Field
         Abstract | Full Text:  HTML   PDF

Unit 2A.2 Total Internal Reflection Fluorescence (TIRF) Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 3C.1 Laboratory Maintenance of Bartonella quintana
         Abstract | Full Text:  HTML   PDF

Unit 8A.1 Growth and Laboratory Maintenance of Campylobacter jejuni
         Abstract | Full Text:  HTML   PDF

Unit 8A.2 Genetic Manipulation of Campylobacter jejuni
         Abstract | Full Text:  HTML   PDF

Unit 9B.1 Animal Models of Listeria Infection
         Abstract | Full Text:  HTML   PDF

Unit 16B.1 Aphid Transmission of Plant Viruses
         Abstract | Full Text:  HTML   PDF

Unit 16G.1 Identification of Viroids by Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF

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  Supplement 9, May 2008

Unit 1G.1 Biological Sand Filters: Low-Cost Bioremediation Technique for Production of Clean Drinking Water
         Abstract | Full Text:  HTML   PDF

Unit 3A.1 Laboratory Maintenance of Ehrlichia chaffeensis and Ehrlichia canis and Recovery of Organisms for Molecular Biology and Proteomics Studies
         Abstract | Full Text:  HTML   PDF

Unit 3A.3 Isolation and Molecular Detection of Ehrlichia from Vertebrate Animals
         Abstract | Full Text:  HTML   PDF

Unit 7B.2 Laboratory Maintenance of Bdellovibrio
         Abstract | Full Text:  HTML   PDF

Unit 12.1 Analysis of Bacterial Membrane Proteins Produced During Mammalian Infection Using Hydrophobic Antigen Tissue Triton Extraction (HATTREX)
         Abstract | Full Text:  HTML   PDF

Unit 13C.1 Laboratory Maintenance and Cultivation of Bacteroides Species
         Abstract | Full Text:  HTML   PDF

Appendix 3D Direct PCR of Intact Bacteria (Colony PCR)
         Abstract | Full Text:  HTML   PDF

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  Supplement 8, February 2008

Unit 2C.2 Atomic Force Microscopy (AFM)
         Abstract | Full Text:  HTML   PDF

Unit 4A.1 Laboratory Maintenance of Neisseria gonorrhoeae
         Abstract | Full Text:  HTML   PDF

Unit 15A.1 Lymphocytic Choriomeningitis Virus (LCMV): Propagation, Quantitation, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 16I.1 Use of Potato Virus X (PVX)–Based Vectors for Gene Expression and Virus-Induced Gene Silencing (VIGS)
         Abstract | Full Text:  HTML   PDF

Appendix 2C Where to Find Recipes for Media
         Full Text:  HTML   PDF

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  Supplement 7, November 2007

Unit 10A.5 Animal Models of M. tuberculosis Infection
         Abstract | Full Text:  HTML   PDF

Unit 12A.1 Isolation and Laboratory Maintenance of Treponema pallidum
         Abstract | Full Text:  HTML   PDF

Unit 16F.1 Cloning of Large Positive-Strand RNA Viruses
         Abstract | Full Text:  HTML   PDF

Unit 16I.4 Using Vectors Derived from Tomato Bushy Stunt Virus (TBSV) and TBSV Defective Interfering RNAs (DIs)
         Abstract | Full Text:  HTML   PDF

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  Supplement 6, August 2007

Unit 1B.5 Growing Oral Biofilms in a Constant Depth Film Fermentor (CDFF)
         Abstract | Full Text:  HTML   PDF

Unit 10A.1 Laboratory Maintenance of Mycobacterium tuberculosis
         Abstract | Full Text:  HTML   PDF

Unit 10A.2 Genetic Manipulation of Mycobacterium tuberculosis
         Abstract | Full Text:  HTML   PDF

Unit 10A.4 Analyses of Mycobacterium tuberculosis Proteins
         Abstract | Full Text:  HTML   PDF

Unit 13B.1 Laboratory Maintenance of Flavobacterium psychrophilum and Flavobacterium columnare
         Abstract | Full Text:  HTML   PDF

Unit 14E.2 Epstein-Barr Virus (EBV): Infection, Propagation, Quantitation, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 16C.1 Reverse Transcription-Polymerase Chain Reaction-Based Detection of Plant Viruses
         Abstract | Full Text:  HTML   PDF

Unit 16E.1 Extracting Viral RNAs from Plant Protoplasts
         Abstract | Full Text:  HTML   PDF

Appendix 4C Isolation of Human Peripheral Blood Mononuclear Cells (PBMCs)
         Abstract | Full Text:  HTML   PDF

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  Supplement 5, May 2007

Unit 1C.3 Detection and Analysis of Quorum-Quenching Enzymes Against Acyl Homoserine Lactone Quorum-Sensing Signals
         Abstract | Full Text:  HTML   PDF

Unit 3A.4 Transfection of Wolbachia pipientis into Drosophila Embryos
         Abstract | Full Text:  HTML   PDF

Unit 5A.1 Interaction of Enterohemorrhagic Escherichia coli (EHEC) with Mammalian Cells: Cell Adhesion, Type III Secretion, and Actin Pedestal Formation
         Abstract | Full Text:  HTML   PDF

Unit 10A.3 Isolation and Analysis of Mycobacterium tuberculosis Mycolic Acids
         Abstract | Full Text:  HTML   PDF

Unit 12E.4 Genetic Manipulation of Leptospira biflexa
         Abstract | Full Text:  HTML   PDF

Unit 13C.2 Genetic Manipulation of Porphyromonas gingivalis
         Abstract | Full Text:  HTML   PDF

Unit 16D.3 Preparation and Electroporation of Oat Protoplasts from Cell Suspension Culture
         Abstract | Full Text:  HTML   PDF

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  Supplement 4, February 2007

Unit 1E.1 Characterization of Bacteria in Mixed Biofilm Communities Using Denaturing Gradient Gel Electrophoresis (DGGE)
         Abstract | Full Text:  HTML   PDF

Unit 9C.4 Tissue Culture Assays Used to Analyze Invasion by Staphylococcus aureus
         Abstract | Full Text:  HTML   PDF

Unit 12C.1 Laboratory Maintenance of Borrelia burgdorferi
         Abstract | Full Text:  HTML   PDF

Unit 16D.2 Preparation and Inoculation of Mesophyll Protoplasts from Monocotyledenous and Dicotyledenous Hosts
         Abstract | Full Text:  HTML   PDF

Unit 16E.3 Northern Analysis of Viral Plus- and Minus-Strand RNAs
         Abstract | Full Text:  HTML   PDF

Unit 16F.3 Rapid Full-Length Cloning of Nonpolyadenylated RNA Virus Genomes
         Abstract | Full Text:  HTML   PDF

Appendix 2C Where to Find Recipes for Media
         Full Text:  HTML   PDF

Appendix 4B Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVEC)
         Abstract | Full Text:  HTML   PDF

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  Supplement 3, November 2006

Unit 1A.2 Biosafety Practices Associated with Potential Agents of Biocrime and Biowarfare
         Abstract | Full Text:  HTML   PDF

Unit 1C.2 LacZ -Based Detection of Acyl-Homoserine Lactone Quorum-Sensing Signals
         Abstract | Full Text:  HTML   PDF

Unit 2B.1 Transmission Electron Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 10B.2 Zebrafish and Frog Models of Mycobacterium marinum Infection
         Abstract | Full Text:  HTML   PDF

Unit 15G.1 Influenza: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

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  Supplement 2, August 2006

Unit 6A.5 Detection, Isolation, and Identification of Vibrio cholerae from the Environment
         Abstract | Full Text:  HTML   PDF

Unit 9D.5 Murine Models of Streptococcus pyogenes Infection
         Abstract | Full Text:  HTML   PDF

Unit 12E.2 Hamster Model of Leptospirosis
         Abstract | Full Text:  HTML   PDF

Unit 15J.1 Human Immunodeficiency Viruses: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Appendix 1A Abbreviations Used in this Manual
         Abstract | Full Text:  HTML   PDF

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  Supplement 1, May 2006

Unit 1A.4 Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF

Unit 2C.1 Basic Confocal Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 9B.4 Tissue Culture Cell Assays Used to Analyze Listeria monocytogenes
         Abstract | Full Text:  HTML   PDF

Unit 15F.2 Newcastle Disease Virus: Propagation, Quantification, and Storage
         Abstract | Full Text:  HTML   PDF

Unit 16B.2 Delivery and Expression of Functional Viral RNA Genomes In Planta by Agroinfiltration
         Abstract | Full Text:  HTML   PDF

Unit 16I.6 Using Viral Vectors to Silence Endogenous Genes
         Abstract | Full Text:  HTML   PDF

Unit 16K.1 In Vitro Translation of Plant Viral RNA
         Abstract | Full Text:  HTML   PDF

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  Core Publication, February 2006

Unit 1A.1 Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms
         Full Text:  HTML   PDF

Unit 1A.3 Safe Use of Hazardous Chemicals
         Abstract | Full Text:  HTML   PDF

Unit 1B.1 Growing and Analyzing Static Biofilms
         Abstract | Full Text:  HTML   PDF

Unit 1B.2 Growing and Analyzing Biofilms in Flow Cells
         Abstract | Full Text:  HTML   PDF

Unit 1B.3 Growing and Analyzing Biofilms in Fermenters
         Abstract | Full Text:  HTML   PDF

Unit 1C.1 Methods for Analysis of Bacterial Autoinducer-2 Production
         Abstract | Full Text:  HTML   PDF

Unit 1D.3 Example of Real-Time Quantitative Reverse Transcription–PCR (Q-RT-PCR) Analysis of Bacterial Gene Expression during Mammalian Infection: Borrelia burgdorferi in Mouse Tissues
         Abstract | Full Text:  HTML   PDF

Unit 1F.2 Proteomic Approaches to Antibiotic Drug Discovery
         Abstract | Full Text:  HTML   PDF

Unit 2A.1 Proper Alignment and Adjustment of the Light Microscope
         Abstract | Full Text:  HTML   PDF

Unit 3A.2 Laboratory Maintenance of Anaplasma phagocytophilum
         Abstract | Full Text:  HTML   PDF

Unit 3B.1 Laboratory Maintenance of Brucella abortus
         Abstract | Full Text:  HTML   PDF

Unit 4A.2 Genetic Manipulation of Neisseria gonorrhoeae
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Unit 5A.2 Rapid Allelic Exchange in Enterohemorrhagic Escherichia coli (EHEC) and Other E. coli Using λ Red Recombination
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Unit 7B.1 Isolation and Classification of Bdellovibrio and Like Organisms
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Unit 8B.1 Laboratory Maintenance of Helicobacter Species
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Unit 10B.1 Laboratory Maintenance of Mycobacterium marinum
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Unit 11A.1 Cultivation and Laboratory Maintenance of Chlamydia trachomatis
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Unit 12B.1 Laboratory Maintenance of Treponema denticola
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Unit 12B.2 Genetic Manipulation of Treponema denticola
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Unit 12C.3 Cultivation of Borrelia burgdorferi in Dialysis Membrane Chambers in Rat Peritonea
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Unit 12E.1 Laboratory Maintenance of Pathogenic Leptospira
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Unit 13A.1 Laboratory Maintenance of Fusobacteria
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Unit 14B.1 Cell Culture Assay for Transient Replication of Human and Animal Papillomaviruses
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Unit 14C.1 Human Adenoviruses: Propagation, Purification, Quantification, and Storage
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Unit 14E.1 Herpes Simplex Virus: Propagation, Quantification, and Storage
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Unit 15B.1 Sindbis Virus: Propagation, Quantification, and Storage
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Unit 16B.3 Inoculation of Plants Using Bombardment
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Unit 16D.1 Callus Cultures of Arabidopsis
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Unit 16D.4 Transfecting Protoplasts by Electroporation to Study Viroid Replication
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Unit 16H.2 Isolation and Cloning of Small RNAs from Virus-Infected Plants
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Unit 16I.5 Using Satellite Tobacco Mosaic Virus Vectors for Gene Silencing
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Unit 16K.2 In Vivo Translation Studies of Plant Viral RNAs Using Reporter Genes
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Appendix 1A Abbreviations Used in this Manual

Appendix 1B Resources for International Biosafety Guidelines and Regulations
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Appendix 2A Commonly Used Reagents
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Appendix 2B Standard Laboratory Equipment
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Appendix 2C Where to Find Recipes for Media

Appendix 3A Assays for Total Protein
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Appendix 3B Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
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Appendix 3C Gram Staining
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Appendix 4A Common Bacterial Culture Techniques and Media
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