Current Protocols in Microbiology
Online ISBN: 9780471729259
DOI: 10.1002/9780471729259
Archive
Current Protocols in Microbiology Supplements
2017: Supplement 44 Supplement 45
2016: Supplement 43 Supplement 42 Supplement 41 Supplement 40
2015: Supplement 39 Supplement 38 Supplement 37 Supplement 36
2014: Supplement 35 Supplement 34 Supplement 33 Supplement 32
2013: Supplement 31 Supplement 30 Supplement 29 Supplement 28
2012: Supplement 27 Supplement 26 Supplement 25 Supplement 24
2011: Supplement 23 Supplement 22 Supplement 21 Supplement 20
2010: Supplement 19 Supplement 18 Supplement 17 Supplement 16
2009: Supplement 15 Supplement 14 Supplement 13 Supplement 12
2008: Supplement 11 Supplement 10 Supplement 9 Supplement 8
2007: Supplement 7 Supplement 6 Supplement 5 Supplement 4
2006: Supplement 3 Supplement 2 Supplement 1 Core publication
Supplement 45, May 2017
Unit 4C.2 Burkholderia thailandensis: Genetic Manipulation
Abstract | Full Text: HTML PDF
Unit 7B.3 Lyophilization of Bdellovibrio bacteriovorus 109J for Long-Term Storage
Abstract | Full Text: HTML PDF
Unit 11A.3 Chlamydia trachomatis Transformation and Allelic Exchange Mutagenesis
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Unit 14B.10 Analysis of Human Papillomavirus Genome Replication Using Two- and Three-Dimensional Agarose Gel Electrophoresis
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Unit 20C.2 Purification Toxoplasma gondii Tissue Cysts Using Percoll Gradients
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Unit 20D.1 Isolation, Culture and Cryopreservation of Sarcocystis species
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Supplement 44, February 2017
Unit 1E.12 Virus Hunting: Discovery of New Episomal Circular Viruses by Rolling Circle Techniques
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Unit 10G.1 Actinomadura Species: Laboratory Maintenance and Ribosome Engineering
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Unit 10H.1Saccharopolyspora Species: Laboratory Maintenance and Enhanced Production of Secondary Metabolites
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Unit 20B.1 Cryptosporidium: Identification and Genetic Typing
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Unit 20C.1 Toxoplasma gondii: Laboratory Maintenance and Growth
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Unit 21A.1 Efficient Genome Editing in the Oomycete Phytophthora sojae Using CRISPR/Cas9
Abstract | Full Text: HTML PDF
Supplement 43, November 2016
Unit 14B.9 Detection and Genotyping of Human Papillomaviruses from Archival Formalin-Fixed Tissue Samples
Abstract | Full Text: HTML PDF
Unit 15D.4 Zika Virus: Quantification, Propagation, Detection, and Storage
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Unit 20A.1 Blastocystis: Isolation, Xenic Cultivation, and Cryopreservation
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Unit 20A.2 Molecular Identification and Subtype Analysis of Blastocystis
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Appendix 4K A Simple and Low-Cost Procedure for Growing Geobacter sulfurreducens Cell Cultures and Biofilms in Bioelectrochemical Systems
Abstract | Full Text: HTML PDF
Supplement 42, August 2016
Unit 4C.1 Burkholderia thailandensis: Growth and Laboratory Maintenance
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Unit 12F.1 Laboratory Cultivation and Maintenance of Borrelia miyamotoi
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Unit 14A.5 Natural Vaccinia Virus Infection: Diagnosis, Isolation, and Characterization
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Appendix 3D Direct PCR of Intact Bacteria (Colony PCR)
Abstract | Full Text: HTML PDF
Supplement 41, May 2016
Unit 1E.11 CLIP-seq to Identify KSHV ORF57-Binding RNA in Host B Cells
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Unit 1F.4 Biochemical Analysis of Microbial Rhodopsins
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Unit 14B.7 Detection of Papillomavirus Gene Expression Patterns in Tissue Sections
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Unit 14B.8 Using Organotypic Epithelial Tissue Culture to Study the Human Papillomavirus Life Cycle
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Unit 14E.7 PA-seq for Global Identification of RNA Polyadenylation Sites of Kaposi's Sarcoma–Associated Herpesvirus Transcripts
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Unit 14G.1 Mimiviruses: Replication, Purification, and Quantification
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Supplement 40, February 2016
Unit 1E.9 Obtaining High Quality DNA from Diverse Clinical Samples
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Unit 1E.10 Investigation of Viral and Host Chromatin by ChIP-PCR or ChIP-Seq Analysis
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Unit 10F.2 Genetic Manipulation of Nocardia Species
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Unit 14B.6 Construction of a Transcription Map for Papillomaviruses using RACE, RNase Protection, and Primer Extension Assays
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Unit 17.8 HT-SPOTi: A Rapid Drug Susceptibility Test (DST) to Evaluate Antibiotic Resistance Profiles and Novel Chemicals for Anti-Infective Drug Discovery
Abstract | Full Text: HTML PDF
Supplement 39, November 2015
Unit 1A.5 Biosafety Oversight and Compliance: What do you Mean, I have to Fill Out Another Form?!
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Unit 3A.6 Electrotransformation and Clonal Isolation of Rickettsia Species
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Unit 7A.1 Myxococcus xanthus Growth, Development, and Isolation
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Unit 10F.1 Laboratory Maintenance of Nocardia Species
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Unit 14A.3 Preparation of Cell Cultures and Vaccinia Virus Stocks
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Unit 14A.4 Generation of Recombinant Vaccinia Viruses
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Supplement 38, August 2015
Unit 6A.6 Infant Rabbit Model for Diarrheal Diseases
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Unit 14B.5 Production of Furin-Cleaved Papillomavirus Pseudovirions and Their Use for In Vitro Neutralization Assays of L1- or L2-Specific Antibodies
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Unit 14F.1 Mouse Polyomavirus: Propagation, Purification, Quantification, and Storage
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Unit 14F.2 In Vitro Replication Assay for Merkel Cell Polyomavirus (MCPyV)
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Unit 19A.1 Detection and Isolation of Epichloë Species, Fungal Endophytes of Grasses
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Supplement 37, May 2015
Unit 1E.8 Isolating Viral and Host RNA Sequences from Archival Material and Production of cDNA Libraries for High-Throughput DNA Sequencing
Abstract | Full Text: HTML PDF
Unit 6F.2 Genetic Manipulation of Stenotrophomonas multiphilia
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Unit 14B.4 Fluorescently Labeled Human Papillomavirus Pseudovirions for Use in Virus Entry Experiments
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Unit 15C.6 Rotaviruses: Extraction and Isolation of RNA, Reassortant Strains, and NSP4 Protein
Abstract | Full Text: HTML PDF
Unit 15E.2 Growth and Quantification of MERS-CoV Infection
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Supplement 36, February 2015
Unit 1E.3 Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms
Abstract | Full Text: HTML PDF
Unit 9D.4 Streptococcus pneumoniae Supragenome Hybridization Arrays for Profiling of Genetic Content and Gene Expression
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Unit 17.9 Aminoglycoside-Enabled Elucidation of Bacterial Persister Metabolism
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Unit 18.1 Progress in Vaccine Development
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Appendix 2C Where to Find Recipes for Media
Abstract | Full Text: HTML PDF
Supplement 35, November 2014
Unit 6A.4 Vibrio cholerae: Measuring Natural Transformation Frequency
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Unit 6G.1 Laboratory Maintenance of Acinetobacter baumannii
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Unit 6G.2 Genetic Manipulation of Acinetobacter baumannii
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Unit 14E.5 Quantitative Analysis of HSV Gene Expression during Lytic Infection
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Unit 14E.6 Analysis of HSV Viral Reactivation in Explants of Sensory Neurons
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Supplement 34, August 2014
Unit 1F.3 Quantitative Proteomic Analysis of the Cell Envelopes and Native Membrane Vesicles Derived from Gram-Negative Bacteria
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Unit 4A.3 Isolation of Cell Envelopes and Naturally Released Membrane Vesicles of Neisseria gonorrhoeae
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Unit 12C.5 Molecular Typing of Borrelia burgdorferi
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Unit 14B.3 Using Organotypic (Raft) Epithelial Tissue Cultures for the Biosynthesis and Isolation of Infectious Human Papillomaviruses
Abstract | Full Text: HTML PDF
Unit 15I.1 Avian Bornaviruses: Diagnosis, Isolation, and Genotyping
Abstract | Full Text: HTML PDF
Supplement 33, May 2014
Unit 1G.1 Biological Sand Filters: Low-Cost Bioremediation Technique for Production of Clean Drinking Water
Abstract | Full Text: HTML PDF
Unit 1G.2 Bioremediation of Turbid SurfaceWater Using Seed Extract from the Moringa oleifera Lam. (Drumstick) Tree
Abstract | Full Text: HTML PDF
Unit 9B.5 Isolation of Listeria monocytogenes from Food and Water: Official and Experimental Protocols
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Unit 14B.2 Analyzing the Human Papillomavirus (HPV) Life Cycle in Primary Keratinocytes with a Quantitative Colony-Forming Assay
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Unit 15K.2 Murine Norovirus: Propagation, Quantification, and Genetic Manipulation
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Unit 18.4 Western Blot Assay for Quantitative and Qualitative Antigen Detection in Vaccine Development
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Supplement 32, February 2014
Unit 1E.7 Gene Identification in Prokaryotic Genomes, Phages, Metagenomes, and EST Sequences with GeneMarkS Suite
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Unit 6F.1 Laboratory Culture and Maintenance of Stenotrophomonas maltophilia
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Unit 9C.3 Genetic Manipulation of Staphylococcus aureus
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Unit 12E.5 Leptospirosis Serodiagnosis by the Microscopic Agglutination Test
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Supplement 31, November 2013
Unit 9B.2 Listeria monocytogenes Cultivation and Laboratory Maintenance
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Unit 9B.3 A Mouse Model of Foodborne Listeria monocytogenes Infection
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Unit 15D.3 Propagation, Quantification, Detection, and Storage of West Nile Virus
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Unit 15K.1 Human Norovirus Detection and Production, Quantification, and Storage of Virus-Like Particles
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Unit 18.3 DNA Immunization
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Appendix 3N Care and Handling of Laboratory Mice
Abstract | Full Text: HTML PDF
Supplement 30, October 2013
Unit 9A.3 Therapeutic Approaches for Clostridium difficile Infections
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Unit 9D.2 Laboratory Growth and Maintenance of Streptococcus pyogenes (The Group A Streptococcus, GAS)
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Unit 9D.3 Genetic Manipulation of Streptococcus pyogenes (The Group A Streptococcus, GAS)
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Unit 15H.3 RNA Structure Analysis of Viruses Using SHAPE
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Unit 18.2 Newcastle Disease Virus-Like Particles: Preparation, Purification, Quantification, and Incorporation of Foreign Glycoproteins
Abstract | Full Text: HTML PDF
Supplement 29, May 2013
Unit 14D.5 Gene Transfer to the CNS Using Recombinant Adeno-Associated Virus
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Unit 14D.6 Gene Transfer in the Liver Using Recombinant Adeno-Associated Virus
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Unit 15G.1 Influenza: Propagation, Quantification, and Storage
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Unit 15G.4 Insertion of a GFP Reporter Gene in Influenza Virus
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Unit 15H.1 Poliovirus: Generation, Quantification, Propagation, Purification, and Storage
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Unit 15H.2 Poliovirus: Generation and Characterization of Mutants
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Supplement 28, February 2013
Unit 1E.6 Analyzing Arthropods for the Presence of Bacteria
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Unit 9C.1 Growth and Laboratory Maintenance of Staphylococcus aureus
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Unit 9C.2 Laboratory Maintenance of Methicillin-Resistant Staphylococcus aureus (MRSA)
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Unit 14D.3 Gene Transfer in Skeletal and Cardiac Muscle Using Recombinant Adeno-Associated Virus
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Unit 14D.4 Retinal Gene Delivery by rAAV and DNA Electroporation
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Unit 15J.1 Human Immunodeficiency Viruses: Propagation, Quantification, and Storage
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Supplement 27, November 2012
Unit 1E.5 Using QIIME to Analyze 16S rRNA Gene Sequences from Microbial Communities
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Unit 2C.5 Use of Image-Based Flow Cytometry in Bacterial Viability Analysis Using Fluorescent Probes
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Unit 3D.4 Isolation and Cultivation of Agrobacterium Species from Natural Sources
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Unit 5A.4 Growth and Maintenance of Escherichia coli Laboratory Strains
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Unit 11A.2 Sphingolipid Trafficking and Purification in Chlamydia trachomatis–Infected Cells
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Unit 15D.2 Dengue Virus: Isolation, Propagation, Quantification, and Storage
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Supplement 26, August 2012
Unit 1F.2 2-D Gel-Based Proteomic Approaches to Antibiotic Drug Discovery
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Unit 6A.5 Detection, Isolation, and Identification of Vibrio cholerae from the Environment
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Unit 7B.1 Isolation and Classification of Bdellovibrio and Like Organisms
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Unit 14D.1 Production and Discovery of Novel Recombinant Adeno-Associated Viral Vectors
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Unit 14D.2 Gene Transfer in the Lung Using Recombinant Adeno-Associated Virus
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Supplement 25, May 2012
Unit 2B.2 Scanning Electron Microscopy
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Unit 3D.2 Genetic Manipulation of Agrobacterium
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Unit 3D.3 Phenotypic Analyses of Agrobacterium
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Unit 6E.1 Growth and Laboratory Maintenance of Pseudomonas aeruginosa
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Unit 15C.5 Separation and Isolation of BTV dsRNA Segments and Viral Proteins
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Supplement 24, February 2012
Unit 1F.1 Identification of Novel DNA-Binding Proteins Using DNA-Affinity Chromatography/Pull Down
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Unit 3D.1 Laboratory Maintenance of Agrobacterium
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Unit 8B.1 Laboratory Maintenance of Helicobacter Species
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Unit 14B.1 Cell Culture Assay for Transient Replication of Human and Animal Papillomaviruses
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Unit 14E.4 Human Cytomegalovirus: Bacterial Artificial Chromosome (BAC) Cloning and Genetic Manipulation
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Unit 15C.4 Bluetongue Virus (BTV): Propagation, Quantification, and Storage
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Supplement 23, November 2011
Unit 1A.3 Safe Use of Hazardous Chemicals
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Unit 1C.1 Methods for Analysis of Bacterial Autoinducer-2 Production
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Unit 4A.2 Genetic Manipulation of Neisseria gonorrhoeae
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Unit 5B.3 Extraction, Purification, and Identification of Yersiniabactin, the Siderophore of Yersinia pestis
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Unit 6A.3 Vibriocidal Assays to Determine the Antibody Titer of Patient Sera Samples
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Supplement 22, August 2011
Unit 1B.1 Growing and Analyzing Static Biofilms
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Unit 6A.2 Genetic Screens and Biochemical Assays to Characterize Vibrio cholerae O1 Biotypes: Classical and El Tor
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Unit 12D.1 Isolation and Maintenance of Brachyspira Species
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Unit 17.6 Laboratory Detection of Vancomycin Nonsusceptible Staphylococcus aureus
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Unit 17.7 Screening of Hepatitis C Virus Inhibitors Using Genotype 1a HCV Replicon Cell Lines
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Appendix 3L Generation of Transformation Competent E. coli
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Appendix 3M SDS-Polyacrylamide gel Electrophoresis (SDS-PAGE) of Proteins
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Supplement 21, May 2011
Unit 1B.2 Growing and Analyzing Biofilms in Flow Chambers
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Unit 2C.3 Whole-Body Imaging of Infection Using Fluorescence
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Unit 2C.4 Whole-Body Imaging of Infection Using Bioluminescence
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Unit 14A.2 Production of Myxoma Virus Gateway Entry and Expression Libraries and Validation of Viral Protein Expression
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Unit 15E.1 Coronaviruses: Propagation, Quantification, Storage, and Construction of Recombinant Mouse Hepatitis Virus
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Supplement 20, February 2011
Unit 1E.4 Preparing DNA Libraries for Multiplexed Paired-End Deep Sequencing for Illumina GA Sequencers
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Unit 9A.2 Genetic Manipulation of Clostridium difficile
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Unit 10A.6 Acid-Fast Staining and Petroff-Hausser Chamber Counting of Mycobacterial Cells in Liquid Suspension
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Unit 12C.4 Genetic Transformation of Borrelia burgdoferi
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Unit 12E.3 Rat Model of Chronic Leptospirosis
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Supplement 19, November 2010
Unit 1D.2 Using Host 28S Ribosomal RNA as a Housekeeping Gene for Quantitative Real-Time Reverse Transcription-PCR (qRT-PCR) in Virus-Infected Animal Cells
Abstract | Full Text: HTML PDF
Unit 1E.2 Simultaneous Isolation of Ixodidae and Bacterial (Borrelia spp.) Genomic DNA
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Unit 1E.3 Genome-Wide Fitness and Genetic Interactions Determined by Tn-seq, a High-Throughput Massively Parallel Sequencing Method for Microorganisms
Abstract | Full Text: HTML PDF
Unit 10E.2 Laboratory Maintenance of Streptomyces argillaceus and Streptomyces griseus
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Unit 10E.3 Genetic Manipulation of Streptomyces Species
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Unit 10E.4 Isolation of Streptomyces Species from Soil
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Unit 15D.1 Hepatitis C Virus: Propagation, Quantification, and Storage
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Unit 16J.1 A High-Throughput Approach for Studying Virus Replication in Yeast
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Supplement 18, August 2010
Unit 6D.1 Laboratory Growth and Maintenance of Haemophilus influenzae
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Unit 10D.1 Laboratory Maintenance of Mycobacterium abscessus
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Unit 10D.2 Genetic Manipulation of Mycobacterium abscessus
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Unit 10E.1 Laboratory Maintenance of Streptomyces Species
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Unit 14E.3 Human Cytomegalovirus: Propagation, Quantification, and Storage
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Unit 17.5 Cell-Based Hepatitis C Virus Infection Fluorescence Resonance Energy Transfer (FRET) Assay for Antiviral Compound Screening
Abstract | Full Text: HTML PDF
Supplement 17, May 2010
Unit 6A.1 Growth and Laboratory Maintenance of Vibrio cholerae
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Unit 14A.1 Myxoma Virus: Propagation, Purification, Quantification, and Storage
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Unit 15F.1 Human Parainfluenza Virus Type 3 (HPIV-3): Construction and Rescue of an Infectious, Recombinant Virus Expressing the Enhanced Green Fluorescent Protein (EGFP)
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Appendix 2C Where to Find Recipes for Media
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Appendix 4H Growth and Maintenance of Baby Hamster Kidney (BHK) Cells
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Appendix 4I Growth and Maintenance of Chick Embryo Fibroblasts (CEK)
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Appendix 4J Growth and Maintenance of Mosquito Cell Lines
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Supplement 16, February 2010
Unit 1G.2 Bioremediation of Turbid Surface Water Using Seed Extract from Moringa oleifera Lam. (Drumstick) Tree
Abstract | Full Text: HTML PDF
Unit 12C.2 Methods of Identifying Membrane Proteins in Spirochetes
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Unit 15B.1 Sindbis Virus: Propagation, Quantification, and Storage
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Unit 17.1 Characterization of the In Vitro Activity of Novel Lipoglycopeptide Antibiotics
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Unit 17.4 Identification of Small-Molecule Scaffolds for P450 Inhibitors
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Supplement 15, November 2009
Unit 1D.1 Example of Use of TaqMan Real-Time RT-PCR to Analyze Gene Transcript Levels: Haemophilus influenzae
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Unit 4B.1 Laboratory Maintenance of Bordetella pertussis
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Unit 6C.1 Laboratory Maintenance of Coxiella burnetii
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Unit 15C.3 Culturing, Storage, and Quantification of Rotaviruses
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Appendix 3C Differential Staining of Bacteria: Gram Stain
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Appendix 3E Preliminary Staining of Bacteria: Simple Stains
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Appendix 3F Preliminary Staining of Bacteria: Negative Stain
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Appendix 3G Differential Staining of Bacteria: Flagella Stain
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Appendix 3H Differential Staining of Bacteria: Acid Fast Stain
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Appendix 3I Differential Staining of Bacteria: Capsule Stain
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Appendix 3J Differential Staining of Bacteria: Endospore Stain
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Appendix 3K Fluorescent Staining of Bacteria: Viability and Antibody Labeling
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Supplement 14, August 2009
Unit 5A.3 Genotyping Escherichia coli O157:H7 for Its Ability to Cause Disease in Humans
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Unit 10C.1 Laboratory Maintenance of Mycobacterium smegmatis
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Unit 15C.1 Mammalian Reoviruses: Propagation, Quantification, and Storage
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Unit 15C.2 Avian Reoviruses: Propagation, Quantification, and Storage
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Unit 16A.1 Growth Conditions for Plant Virus–Host Studies
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Unit 16A.2 Propagation of Major Plant-Virus Hosts
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Appendix 4G Growth and Maintenance of Quail Fibrosarcoma QM5 Cells
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Supplement 13, May 2009
Unit 1A.1 Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms
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Unit 9D.1 Adult Zebrafish Model of Streptococcal Infection
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Unit 15G.2 The Ferret Model for Influenza
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Unit 15G.3 The Mouse Model for Influenza
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Unit 16B.6 Mechanical Inoculation of Plant Viruses
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Supplement 12, February 2009
Unit 9A.1 Laboratory Maintenance of Clostridium difficile
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Unit 11B.1 Cultivation and Laboratory Maintenance of Chlamydia pneumoniae
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Unit 16B.4 Fungal Transmission of Plant Viruses
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Unit 16B.5 Testing of Transmission of Tobraviruses by Nematodes
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Unit 17.2 Structure-Based Approaches to Antibiotic Drug Discovery
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Unit 17.3 Novel Approaches to Bacterial Infection Therapy by Interfering with Cell-to-Cell Signaling
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Appendix 4F Anaerobic Cell Culture
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Supplement 11, November 2008
Unit 3A.5 Laboratory Maintenance of Rickettsia rickettsii
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Unit 5B.1 Laboratory Maintenance and Characterization of Yersinia pestis
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Unit 5B.2 Isolation and Confirmation of Yersinia pestis Mutants Exempt from Select Agent Regulations
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Unit 6B.1 Laboratory Maintenance of Moraxella catarrhalis
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Unit 8A.3 Experimental Chick Colonization by Campylobacter jejuni
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Appendix 2C Where to Find Recipes for Media
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Appendix 4D Aseptic Technique
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Appendix 4E Growth and Maintenance of Vero Cell Lines
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Supplement 10, August 2008
Unit 1B.4 Preparing for Biofilm Studies in the Field
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Unit 2A.2 Total Internal Reflection Fluorescence (TIRF) Microscopy
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Unit 3C.1 Laboratory Maintenance of Bartonella quintana
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Unit 8A.1 Growth and Laboratory Maintenance of Campylobacter jejuni
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Unit 8A.2 Genetic Manipulation of Campylobacter jejuni
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Unit 9B.1 Animal Models of Listeria Infection
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Unit 16B.1 Aphid Transmission of Plant Viruses
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Unit 16G.1 Identification of Viroids by Gel Electrophoresis
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Supplement 9, May 2008
Unit 1G.1 Biological Sand Filters: Low-Cost Bioremediation Technique for Production of Clean Drinking Water
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Unit 3A.1 Laboratory Maintenance of Ehrlichia chaffeensis and Ehrlichia canis and Recovery of Organisms for Molecular Biology and Proteomics Studies
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Unit 3A.3 Isolation and Molecular Detection of Ehrlichia from Vertebrate Animals
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Unit 7B.2 Laboratory Maintenance of Bdellovibrio
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Unit 12.1 Analysis of Bacterial Membrane Proteins Produced During Mammalian Infection Using Hydrophobic Antigen Tissue Triton Extraction (HATTREX)
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Unit 13C.1 Laboratory Maintenance and Cultivation of Bacteroides Species
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Appendix 3D Direct PCR of Intact Bacteria (Colony PCR)
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Supplement 8, February 2008
Unit 2C.2 Atomic Force Microscopy (AFM)
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Unit 4A.1 Laboratory Maintenance of Neisseria gonorrhoeae
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Unit 15A.1 Lymphocytic Choriomeningitis Virus (LCMV): Propagation, Quantitation, and Storage
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Unit 16I.1 Use of Potato Virus X (PVX)–Based Vectors for Gene Expression and Virus-Induced Gene Silencing (VIGS)
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Appendix 2C Where to Find Recipes for Media
Full Text: HTML PDF
Supplement 7, November 2007
Unit 10A.5 Animal Models of M. tuberculosis Infection
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Unit 12A.1 Isolation and Laboratory Maintenance of Treponema pallidum
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Unit 16F.1 Cloning of Large Positive-Strand RNA Viruses
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Unit 16I.4 Using Vectors Derived from Tomato Bushy Stunt Virus (TBSV) and TBSV Defective Interfering RNAs (DIs)
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Supplement 6, August 2007
Unit 1B.5 Growing Oral Biofilms in a Constant Depth Film Fermentor (CDFF)
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Unit 10A.1 Laboratory Maintenance of Mycobacterium tuberculosis
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Unit 10A.2 Genetic Manipulation of Mycobacterium tuberculosis
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Unit 10A.4 Analyses of Mycobacterium tuberculosis Proteins
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Unit 13B.1 Laboratory Maintenance of Flavobacterium psychrophilum and Flavobacterium columnare
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Unit 14E.2 Epstein-Barr Virus (EBV): Infection, Propagation, Quantitation, and Storage
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Unit 16C.1 Reverse Transcription-Polymerase Chain Reaction-Based Detection of Plant Viruses
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Unit 16E.1 Extracting Viral RNAs from Plant Protoplasts
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Appendix 4C Isolation of Human Peripheral Blood Mononuclear Cells (PBMCs)
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Supplement 5, May 2007
Unit 1C.3 Detection and Analysis of Quorum-Quenching Enzymes Against Acyl Homoserine Lactone Quorum-Sensing Signals
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Unit 3A.4 Transfection of Wolbachia pipientis into Drosophila Embryos
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Unit 5A.1 Interaction of Enterohemorrhagic Escherichia coli (EHEC) with Mammalian Cells: Cell Adhesion, Type III Secretion, and Actin Pedestal Formation
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Unit 10A.3 Isolation and Analysis of Mycobacterium tuberculosis Mycolic Acids
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Unit 12E.4 Genetic Manipulation of Leptospira biflexa
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Unit 13C.2 Genetic Manipulation of Porphyromonas gingivalis
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Unit 16D.3 Preparation and Electroporation of Oat Protoplasts from Cell Suspension Culture
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Supplement 4, February 2007
Unit 1E.1 Characterization of Bacteria in Mixed Biofilm Communities Using Denaturing Gradient Gel Electrophoresis (DGGE)
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Unit 9C.4 Tissue Culture Assays Used to Analyze Invasion by Staphylococcus aureus
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Unit 12C.1 Laboratory Maintenance of Borrelia burgdorferi
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Unit 16D.2 Preparation and Inoculation of Mesophyll Protoplasts from Monocotyledenous and Dicotyledenous Hosts
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Unit 16E.3 Northern Analysis of Viral Plus- and Minus-Strand RNAs
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Unit 16F.3 Rapid Full-Length Cloning of Nonpolyadenylated RNA Virus Genomes
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Appendix 2C Where to Find Recipes for Media
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Appendix 4B Isolation and Culture of Human Umbilical Vein Endothelial Cells (HUVEC)
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Supplement 3, November 2006
Unit 1A.2 Biosafety Practices Associated with Potential Agents of Biocrime and Biowarfare
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Unit 1C.2 LacZ -Based Detection of Acyl-Homoserine Lactone Quorum-Sensing Signals
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Unit 2B.1 Transmission Electron Microscopy
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Unit 10B.2 Zebrafish and Frog Models of Mycobacterium marinum Infection
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Unit 15G.1 Influenza: Propagation, Quantification, and Storage
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Supplement 2, August 2006
Unit 6A.5 Detection, Isolation, and Identification of Vibrio cholerae from the Environment
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Unit 9D.5 Murine Models of Streptococcus pyogenes Infection
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Unit 12E.2 Hamster Model of Leptospirosis
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Unit 15J.1 Human Immunodeficiency Viruses: Propagation, Quantification, and Storage
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Appendix 1A Abbreviations Used in this Manual
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Supplement 1, May 2006
Unit 1A.4 Safe Use of Radioisotopes
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Unit 2C.1 Basic Confocal Microscopy
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Unit 9B.4 Tissue Culture Cell Assays Used to Analyze Listeria monocytogenes
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Unit 15F.2 Newcastle Disease Virus: Propagation, Quantification, and Storage
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Unit 16B.2 Delivery and Expression of Functional Viral RNA Genomes In Planta by Agroinfiltration
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Unit 16I.6 Using Viral Vectors to Silence Endogenous Genes
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Unit 16K.1 In Vitro Translation of Plant Viral RNA
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Core Publication, February 2006
Unit 1A.1 Biosafety: Guidelines for Working with Pathogenic and Infectious Microorganisms
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Unit 1A.3 Safe Use of Hazardous Chemicals
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Unit 1B.1 Growing and Analyzing Static Biofilms
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Unit 1B.2 Growing and Analyzing Biofilms in Flow Cells
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Unit 1B.3 Growing and Analyzing Biofilms in Fermenters
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Unit 1C.1 Methods for Analysis of Bacterial Autoinducer-2 Production
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Unit 1D.3 Example of Real-Time Quantitative Reverse Transcription–PCR (Q-RT-PCR) Analysis of Bacterial Gene Expression during Mammalian Infection: Borrelia burgdorferi in Mouse Tissues
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Unit 1F.2 Proteomic Approaches to Antibiotic Drug Discovery
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Unit 2A.1 Proper Alignment and Adjustment of the Light Microscope
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Unit 3A.2 Laboratory Maintenance of Anaplasma phagocytophilum
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Unit 3B.1 Laboratory Maintenance of Brucella abortus
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Unit 4A.2 Genetic Manipulation of Neisseria gonorrhoeae
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Unit 5A.2 Rapid Allelic Exchange in Enterohemorrhagic Escherichia coli (EHEC) and Other E. coli Using λ Red Recombination
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Unit 7B.1 Isolation and Classification of Bdellovibrio and Like Organisms
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Unit 8B.1 Laboratory Maintenance of Helicobacter Species
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Unit 10B.1 Laboratory Maintenance of Mycobacterium marinum
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Unit 11A.1 Cultivation and Laboratory Maintenance of Chlamydia trachomatis
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Unit 12B.1 Laboratory Maintenance of Treponema denticola
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Unit 12B.2 Genetic Manipulation of Treponema denticola
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Unit 12C.3 Cultivation of Borrelia burgdorferi in Dialysis Membrane Chambers in Rat Peritonea
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Unit 12E.1 Laboratory Maintenance of Pathogenic Leptospira
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Unit 13A.1 Laboratory Maintenance of Fusobacteria
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Unit 14B.1 Cell Culture Assay for Transient Replication of Human and Animal Papillomaviruses
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Unit 14C.1 Human Adenoviruses: Propagation, Purification, Quantification, and Storage
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Unit 14E.1 Herpes Simplex Virus: Propagation, Quantification, and Storage
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Unit 15B.1 Sindbis Virus: Propagation, Quantification, and Storage
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Unit 16B.3 Inoculation of Plants Using Bombardment
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Unit 16D.1 Callus Cultures of Arabidopsis
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Unit 16D.4 Transfecting Protoplasts by Electroporation to Study Viroid Replication
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Unit 16H.2 Isolation and Cloning of Small RNAs from Virus-Infected Plants
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Unit 16I.5 Using Satellite Tobacco Mosaic Virus Vectors for Gene Silencing
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Unit 16K.2 In Vivo Translation Studies of Plant Viral RNAs Using Reporter Genes
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Appendix 1A Abbreviations Used in this Manual
Appendix 1B Resources for International Biosafety Guidelines and Regulations
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Appendix 2A Commonly Used Reagents
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Appendix 2B Standard Laboratory Equipment
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Appendix 2C Where to Find Recipes for Media
Appendix 3A Assays for Total Protein
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Appendix 3B Diagnosis and Treatment of Mycoplasma-Contaminated Cell Cultures
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Appendix 3C Gram Staining
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Appendix 4A Common Bacterial Culture Techniques and Media
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