Primary and Stem Cells: Gene Transfer Technologies and Applications

Primary and Stem Cells: Gene Transfer Technologies and Applications

Editor(s): Uma Lakshmipathy, Bhaskar Thyagarajan

Published Online: 25 OCT 2011 01:54PM EST

Print ISBN: 9780470610749

Online ISBN: 9781118147177

DOI: 10.1002/9781118147177

About this Book

This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications.

Table of contents

    1. You have free access to this content
    1. You have free access to this content
  1. Part I: Cloning and Gene Delivery

    1. Chapter 1

      DNA Assembly Technologies Based on Homologous Recombination (pages 1–17)

      Billyana Tsvetanova, Lansha Peng, Xiquan Liang, Ke Li, Jian-Ping Yang, Tony Ho, Josh Shirley, Liewei Xu, Jason Potter, Wieslaw Kudlicki, Todd Peterson and Federico Katzen

  2. Part II: Nonintegrating Technologies

  3. Part III: Integrating Technologies

    1. Chapter 8

      Sleeping Beauty Transposon-Mediated Stable Gene Delivery (pages 135–161)

      Andrew Wilber, Xianzheng Zhou, Xin Huang, Dan S. Kaufman and R. Scott McIvor

  4. Part IV: Applications

    1. You have free access to this content

SEARCH