Handbook of RNA Biochemistry

Handbook of RNA Biochemistry

Editor(s): Roland K. Hartmann, Albrecht Bindereif, Astrid Schön, Eric Westhof

Published Online: 5 FEB 2008

Print ISBN: 9783527308262

Online ISBN: 9783527619504

DOI: 10.1002/9783527619504

About this Book

This all-in-one compendium of current laboratory techniques for the study of RNA brings together numerous novel methods that were hitherto only available through the primary literature. The editors - themselves experienced RNA researchers - have gathered expert authors from the US and Europe for the ultimate in everyday working knowledge. In more than 300 detailed protocols, all the techniques covered are treated with the user in mind, including practical advice and an invaluable "troubleshooting" section.
The broad scope of the book allows the side-by-side treatment of specialized methods with basic biochemical techniques for the isolation, characterization and functional analysis of RNA. All pertinent laboratory methods are presented in detail, including
+ RNA synthesis, modification and ligation
+ Sequence and structure determination of RNA
+ Isolation and purification of ribonucleoproteins
+ RNA crystallization
+ Single molecule studies on RNA
+ Analysis of splice variants
+ RNA imaging in vivo
+ Modeling and prediction of RNA structure
+ RNA microarrays
+ Aptamer libraries and SELEX technology
+ RNA interference
In short, a guide to successful experimentation for molecular biologists, biochemists and biotechnologists and of interest to every researcher experimenting with RNA.

Table of contents

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  1. Part I: RNA Synthesis - Enzymatic RNA Synthesis, Ligation and Modification

    1. Chapter 1

      Enzymatic RNA Synthesis using Bacteriophage T7 RNA Polymerase (pages 3–21)

      Heike Gruegelsiepe, Astrid Schön, Leif A. Kirsebom and Roland K. Hartmann

    2. Chapter 2

      Production of RNAs with Homogeneous 5′ and 3′ Ends (pages 22–35)

      Mario Mörl, Esther Lizano, Dagmar K. Willkomm and Roland K. Hartmann

    3. Chapter 4

      T4 RNA Ligase (pages 53–74)

      Tina Persson, Dagmar K. Willkomm and Roland K. Hartmann

  2. Part I: RNA Synthesis - Chemical RNA Synthesis

  3. Part II: Structure Determination - Molecular Biology Methods

    1. Chapter 10

      Probing RNA Structures with Enzymes and Chemicals In Vitro and In Vivo (pages 151–171)

      Eric Huntzinger, Maria Possedko, Flore Winter, Hervé Moine, Chantal Ehresmann and Pascale Romby

  4. Part II: Structure Determination - Biophysical Methods

    1. Chapter 26

      Sedimentation Analysis of Ribonucleoprotein Complexes (pages 428–437)

      Jan Medenbach, Andrey Damianov, Silke Schreiner and Albrecht Bindereif

    2. Chapter 27

      Preparation and Handling of RNA Crystals (pages 438–452)

      Boris François, Aurélie Lescoute-Phillips, Andreas Werner and Benoît Masquida

  5. Part II: Structure Determination - Fluorescence and Single Molecule Studies

  6. Part III: RNA Genomics and Bioinformatics - Fluorescence and Single Molecule Studies

  7. Part IV: Analysis of RNA Function 665 - RNA-Protein Interactions in vitro

    1. Chapter 40

      RNA-Protein Interactions in vitro (pages 666–675)

      Steffen Schiffer, Sylvia Rösch, Bettina Späth, Markus Englert, Hildburg Beier and Anita Marchfelder

  8. Part IV: Analysis of RNA Function 665 - RNA-Protein Interactions in vivo

    1. Chapter 46

      Analysis of Alternative Splicing In Vivo using Minigenes (pages 755–782)

      Yesheng Tang, Tatyana Novoyatleva, Natalya Benderska, Shivendra Kishore, Alphonse Thanaraj and Stefan Stamm

  9. Part IV: Analysis of RNA Function 665 - SELEX

    1. Chapter 47

      Artificial Selection: Finding Function amongst Randomized Sequences (pages 783–806)

      Ico de Zwart, Catherine Lozupone, Rob Knight, Amanda Birmingham, Mali Illangasekare, Vasant Jadhav, Michal Legiewicz, Irene Majerfeld, Jeremy Widmann and Michael Yarus

    2. Chapter 50

      In Vitro Selection against Small Targets (pages 853–877)

      Dirk Eulberg, Christian Maasch, Werner G. Purschke and Sven Klussmann

  10. Part IV: RNAi - SELEX

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    1. You have free access to this content