Biotechnology: Bioprocessing, Volume 3, Second Edition

Copyright © 1993 VCH Verlagsgesellschaft mbH

Biotechnology: Bioprocessing, Volume 3, Second Edition

Editor(s): H.-J. Rehm, G. Reed

Published Online: 26 MAY 2008

Print ISBN: 9783527283132

Online ISBN: 9783527620845

DOI: 10.1002/9783527620845

About this Book

Product Information

About The Product

Bioprocessing: an exciting new engineering discipline. It combines the development and optimization of biotechnological processes with effective strategies to recover and purify the desired products. Safety as well as cost play an important role here.
This volume covers the immensely differentiated spectrum of techniques and operations of bioprocessing, presented by the most competent experts in the field. An overview of upstream and downstream processing is given, fermentation and cell culture processes and the design of microbial fermenters are presented. A closing group of chapters is dedicated to issues of process validation, measurement, and regulation.
Topics included are: Industrial Cell Cultures/ Pharmaceutical Proteins/ Bioreactors/ Media and Air Sterilization/ Oxygen Transfer/ Scale Implications/ Fermentation Data Analysis/ Cell and Debris Removal/ Protein Purification/ Electrokinetic Separations/ Final Recovery Steps/ Process Validation

Table of contents

    1. You have free access to this content
      Frontmatter (pages i–xv)

    1. Introduction (pages 1–3)

      Gregory Stephanopoulos and G. Stephanopoulos

  3. Part I: Nature and Issues of Bioprocessing

    1. Chapter 1

      Fermentation: An Overview (pages 7–22)

      Barry C. Buckland and Malcolm D. Lilly

    2. Chapter 2

      Industrial Animal Cell Culture (pages 23–38)

      Brian D. Kelley, Tzyy-Wen Chiou, Morris Rosenberg and Daniel I. C. Wang

    3. Chapter 3

      Overview of Downstream Processing (pages 39–55)

      Ron Spears

    4. Chapter 4

      Proteins and Peptides as Drugs: Sources and Methods of Purification (pages 57–73)

      Stuart E. Builder, Robert L. Garnick, James C. Hodgdon and John R. Ogez

  4. Part II: Product Formation (Upstream Processing)

    1. Chapter 5

      Bioreactors: Description and Modelling (pages 77–104)

      Jens Nielsen and John Villadsen

    2. Chapter 6

      Cell Culture Bioreactors (pages 105–126)

      Athanassios Sambanis and Wei-Shou Hu

    3. Chapter 7

      Media for Microbial Fermentations (pages 127–139)

      Randolph L. Greasham

    4. Chapter 8

      Media for Cell Culture (pages 141–156)

      R. A. Wove

    5. Chapter 9

      Media and Air Sterilization (pages 157–184)

      Gokaraju K. Raju and Charles L. Cooney

    6. Chapter 10

      Oxygen Transfer and Mixing: Scale-Up Implications (pages 185–217)

      Matthias Reuss

    7. Chapter 11

      Aeration in Cell Culture Bioreactors (pages 219–281)

      John G. Aunins and Hans-Jürgen Henzler

    8. Chapter 12

      Strategies for Fermentation with Recombinant Organisms (pages 283–294)

      Tadayuki Imanaka

    9. Chapter 13

      Anaerobic Fermentations (pages 295–318)

      Larry E. Erickson, Daniel Y. C. Fung and Pravate Tuitemwong

    10. Chapter 14

      Fermentation Monitoring and Control (pages 319–354)

      Thomas Chattaway, Gary A. Montague and A. Julian Morris

    11. Chapter 15

      Fermentation Data Analysis for Diagnosis and Control (pages 355–400)

      Gregory Stephanopoulos, Konstantin Konstantinov, Urs Saner and Toshiomi Yoshida

    12. Chapter 16

      Design of Aseptic, Aerated Fermentors (pages 401–426)

      Marvin Charles and Jack Wilson

    13. Chapter 17

      Biotransformations and Enzyme Reactors (pages 427–466)

      Andreas S. Bommarius

  5. Part III: Product Recovery and Purification

    1. Chapter 18

      Cell and Cell Debris Removal: Centrif Ugation and Crossflow Filtration (pages 469–503)

      Rajiv V. Datar and Carl-Gustaf Rosén

    2. Chapter 19

      Cell Disruption and Isolation of Non-Secreted Products (pages 505–526)

      Horst Schütte and Maria-Regina Kula

    3. Chapter 20

      In vitro Protein Refolding (pages 527–555)

      Jeffrey L. Cleland and Daniel I. C. Wang

    4. Chapter 21

      Liquid-Liquid Extraction (Small Molecules) (pages 557–592)

      Karl Schügerl

    5. Chapter 22

      Protein Purification by Liquid-Liquid Extraction (pages 593–616)

      Brian D. Kelley and T. Alan Hatton

    6. Chapter 23

      Protein Separation and Purification (pages 617–642)

      Jan-Christer Janson and Lars Rydén

    7. Chapter 24

      Affinity Separations (pages 643–677)

      Srikanth Sundaram and Martin L. Yarmush

    8. Chapter 25

      Electrokinetic Separations (pages 679–693)

      Alan J. Grodzinsky and Martin L. Yarmush

    9. Chapter 26

      Final Recovery Steps: Lyophilization, Spray-Drying (pages 695–714)

      Christian F. Gölker

  6. Part IV: Process Validation, Regulatory Issues Product Recovery and Purification

    1. Chapter 27

      Analytical Protein Chemistry (pages 717–737)

      Srikanth Sundaram, David M. Yarmush and Martin L. Yarmush

  7. Part IV: Process Validation, Regulatory Issues

    1. Chapter 28

      Biotechnology Facility Design and Process Validation (pages 739–767)

      Michael G. Beatrice

    2. Chapter 29

      Treatment of Biological Waste (pages 769–787)

      Daniel F. Liberman

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      Index (pages 789–816)

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