Cell-Free Protein Synthesis: Methods and Protocols

Copyright © 2008 Wiley-VCH Verlag GmbH & Co. KGaA

Cell-Free Protein Synthesis: Methods and Protocols

Editor(s): Alexander S. Spirin, James R. Swartz

Published Online: 14 JUL 2008

Print ISBN: 9783527316496

Online ISBN: 9783527622702

DOI: 10.1002/9783527622702

About this Book

Product Information

About The Product

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Table of contents

    1. You have free access to this content
      Front Matter (pages i–xx)

    2. Chapter 1

      Cell-Free Protein Synthesis Systems: Historical Landmarks, Classification, and General Methods (pages 1–34)

      Professor Dr. Alexander S. Spirin and Professor Dr. James R. Swartz

    3. Chapter 2

      The Constructive Approach for Cell-Free Translation (pages 35–50)

      Takuya Ueda

    4. Chapter 3

      Functional Genomic Analysis using Sequential Cell-Free Protein Synthesis (pages 51–67)

      Kim A. Woodrow and Professor Dr. James R. Swartz

    5. Chapter 4

      Cell-Free Technology for Rapid Production of Patient-Specific Fusion Protein Vaccines (pages 69–82)

      Aaron R. Goerke, Junhao Yang, Gregory Kanter, Ronald Levy and Professor Dr. James R. Swartz

    6. Chapter 5

      Bacterial Cell-Free System for Highly Efficient Protein Synthesis (pages 83–97)

      Takanori Kigawa, Takayoshi Matsuda, Takashi Yabuki and Shigeyuki Yokoyama

    7. Chapter 6

      The Use of the Escherichia coli Cell-Free Protein Synthesis for Structural Biology and Structural Proteomics (pages 99–109)

      Takanori Kigawa, Makoto Inoue, Masaaki Aoki, Takayoshi Matsuda, Takashi Yabuki, Eiko Seki, Takushi Harada, Satoru Watanabe and Shigeyuki Yokoyama

    8. Chapter 7

      The Wheat Germ Cell-Free Protein Synthesis System (pages 111–139)

      Tatsuya Sawasaki and Yaeta Endo

    9. Chapter 8

      Cell-Free Expression of Integral Membrane Proteins for Structural Studies (pages 141–164)

      Christian Klammt, Daniel Schwarz, Ines Lehner, Solmaz Sobhanifar, Frank Löhr, Johan Zeelen, Clemens Glaubitz, Volker Dötsch and Frank Bernhard

    10. Chapter 9

      Cell-Free Production of Membrane Proteins in the Presence of Detergents (pages 165–178)

      Jean-Michel Betton and Marika Miot

    11. Chapter 10

      Novel Techniques using PCR and Cell-Free Protein Synthesis Systems for Combinatorial Bioengineering (pages 179–189)

      Hideo Nakano and Tsuneo Yamane

    12. Chapter 11

      Gene Cloning and Expression in Molecular Colonies (pages 191–206)

      Alexander B. Chetverin, Timur R. Samatov and Helena V. Chetverina

    13. Chapter 12

      Large-Scale Batch Reactions for Cell-Free Protein Synthesis (pages 207–235)

      Alexei M. Voloshin and Professor Dr. James R. Swartz

    14. You have free access to this content
      Index (pages 237–242)

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