Cell-Free Protein Synthesis: Methods and Protocols

Cell-Free Protein Synthesis: Methods and Protocols

Editor(s): Alexander S. Spirin, James R. Swartz

Published Online: 14 JUL 2008

Print ISBN: 9783527316496

Online ISBN: 9783527622702

DOI: 10.1002/9783527622702

About this Book

With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.

Table of contents

    1. You have free access to this content
    2. Chapter 4

      Cell-Free Technology for Rapid Production of Patient-Specific Fusion Protein Vaccines (pages 69–82)

      Aaron R. Goerke, Junhao Yang, Gregory Kanter, Ronald Levy and Professor Dr. James R. Swartz

    3. Chapter 6

      The Use of the Escherichia coli Cell-Free Protein Synthesis for Structural Biology and Structural Proteomics (pages 99–109)

      Takanori Kigawa, Makoto Inoue, Masaaki Aoki, Takayoshi Matsuda, Takashi Yabuki, Eiko Seki, Takushi Harada, Satoru Watanabe and Shigeyuki Yokoyama

    4. Chapter 8

      Cell-Free Expression of Integral Membrane Proteins for Structural Studies (pages 141–164)

      Christian Klammt, Daniel Schwarz, Ines Lehner, Solmaz Sobhanifar, Frank Löhr, Johan Zeelen, Clemens Glaubitz, Volker Dötsch and Frank Bernhard

    5. Chapter 11

      Gene Cloning and Expression in Molecular Colonies (pages 191–206)

      Alexander B. Chetverin, Timur R. Samatov and Helena V. Chetverina

    6. You have free access to this content