Protein Engineering Handbook, Volume 1 & Volume 2

Copyright © 2009 Wiley-VCH Verlag GmbH & Co. KGaA

Protein Engineering Handbook, Volume 1 & Volume 2

Editor(s): Stefan Lutz, Uwe T. Bornscheuer

Published Online: 9 MAR 2011 12:30PM EST

Print ISBN: 9783527318506

Online ISBN: 9783527634026

DOI: 10.1002/9783527634026

About this Book

Product Information

About The Product

Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.

Table of contents

    1. You have free access to this content
      Front Matter (pages I–XLI)

    2. Chapter 1

      Guidelines for the Functional Analysis of Engineered and Mutant Enzymes (pages 1–13)

      Dale E. Edmondson and Giovanni Gadda

    3. Chapter 2

      Engineering Enantioselectivity in Enzyme-Catalyzed Reactions (pages 15–46)

      Romas Kazlauskas

    4. Chapter 3

      Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes (pages 47–79)

      Stefanie Jonas and Florian Hollfelder

    5. Chapter 4

      Φ-Value Analysis of Protein Folding Transition States (pages 81–120)

      Neil Ferguson and Alan R. Fersht

    6. Chapter 5

      Protein Folding and Solubility: Pathways and High-Throughput Assays (pages 121–145)

      Adam C. Fisher, Thomas J. Mansell and Matthew P. DeLisa

    7. Chapter 6

      Protein Dynamics and the Evolution of Novel Protein Function (pages 147–185)

      Jörg Zimmermann, Megan C. Thielges, Wayne Yu and Floyd E. Romesberg

    8. Chapter 7

      Gaining Insight into Enzyme Function through Correlation with Protein Motions (pages 187–211)

      Nicolas Doucet and Joelle N. Pelletier

    9. Chapter 8

      Structural Frameworks Suitable for Engineering (pages 213–232)

      Birte Höcker

    10. Chapter 9

      Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space (pages 233–269)

      Ana Beloqui, Miren Zumárraga, Miguel Alcalde, Peter N. Golyshin and Manuel Ferrer

    11. Chapter 10

      Inteins in Protein Engineering (pages 271–293)

      Alison R. Gillies and David W. Wood

    12. Chapter 11

      From Prospecting to Product – Industrial Metagenomics Is Coming of Age (pages 295–323)

      Jürgen Eck, Esther Gabor, Klaus Liebeton, Guido Meurer and Frank Niehaus

    13. Chapter 12

      Computational Protein Design (pages 325–342)

      Jeffery G. Saven

    14. Chapter 13

      Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts (pages 343–362)

      Kevin K. Desai and Brian G. Miller

    15. Chapter 14

      Designing Programmable Protein Switches (pages 363–389)

      Martin Sagermann

    16. Chapter 15

      The Cyclization of Peptides and Proteins with Inteins (pages 391–407)

      Blaise R. Boles and Alexander R. Horswill

    17. Chapter 16

      A Method for Rapid Directed Evolution (pages 409–439)

      Manfred T. Reetz

    18. Chapter 17

      Evolution of Enantioselective Bacillus subtilis Lipase (pages 441–451)

      Thorsten Eggert, Susanne A. Funke, Jennifer N. Andexer, Manfred T. Reetz and Karl-Erich Jaeger

    19. Chapter 18

      Circular Permutation of Proteins (pages 453–471)

      Glenna E. Meister, Manu Kanwar and Marc Ostermeier

    20. Chapter 19

      Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): A Versatile Tool for Generating Targeted Libraries (pages 473–480)

      Asael Herman and Dan S. Tawfik

    21. Chapter 20

      Protein Engineering by Structure-Guided SCHEMA Recombination (pages 481–492)

      Gloria Saab-Rincon, Yougen Li, Michelle Meyer, Martina Carbone, Marco Landwehr and Frances H. Arnold

    22. Chapter 21

      Chimeragenesis in Protein Engineering (pages 493–514)

      Manuela Trani and Prof. Dr. Stefan Lutz

    23. Chapter 22

      Protein Generation Using a Reconstituted System (pages 515–535)

      Bei-Wen Ying and Takuya Ueda

    24. Chapter 23

      Equipping in vivo Selection Systems with Tunable Stringency (pages 537–561)

      Martin Neuenschwander, Andreas C. Kleeb, Peter Kast and Donald Hilvert

    25. Chapter 24

      Protein Engineering by Phage Display (pages 563–603)

      Agathe Urvoas, Philippe Minard and Patrice Soumillion

    26. Chapter 25

      Screening Methodologies for Glycosidic Bond Formation (pages 605–620)

      Amir Aharoni and Stephen G. Withers

    27. Chapter 26

      Yeast Surface Display in Protein Engineering and Analysis (pages 621–648)

      Benjamin J. Hackel and Dane Wittrup

    28. Chapter 27

      In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries (pages 649–667)

      Amir Aharoni and Dan S. Tawfik

    29. Chapter 28

      Colorimetric and Fluorescence-Based Screening (pages 669–711)

      Jean-Louis Reymond

    30. Chapter 29

      Confocal and Conventional Fluorescence-Based High Throughput Screening in Protein Engineering (pages 713–751)

      Ulrich Haupts, Oliver Hesse, Michael Strerath, Peter J. Walla and Wayne M. Coco

    31. Chapter 30

      Alteration of Substrate Specificity and Stereoselectivity of Lipases and Esterases (pages 753–775)

      Dominique Böttcher, Marlen Schmidt and Uwe T. Bornscheuer

    32. Chapter 31

      Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering (pages 777–796)

      Matthew DeSieno, Jing Du and Huimin Zhao

    33. Chapter 32

      Protein Engineering of Modular Polyketide Synthases (pages 797–827)

      Alice Y. Chen and Chaitan Khosla

    34. Chapter 33

      Cyanophycin Synthetases (pages 829–848)

      Anna Steinle and Alexander Steinbüchel

    35. Chapter 34

      Biosynthetic Pathway Engineering Strategies (pages 849–876)

      Claudia Schmidt-Dannert and Alexander Pisarchik

    36. Chapter 35

      Natural Polyester-Related Proteins: Structure, Function, Evolution and Engineering (pages 877–914)

      Seiichi Taguchi and Takeharu Tsuge

    37. Chapter 36

      Bioengineering of Sequence-Repetitive Polypeptides: Synthetic Routes to Protein-Based Materials of Novel Structure and Function (pages 915–938)

      Sonha C. Payne, Melissa Patterson and Vincent P. Conticello

    38. Chapter 37

      Silk Proteins – Biomaterials and Bioengineering (pages 939–959)

      Xiaoqin Wang, Peggy Cebe and David. L. Kaplan

    39. You have free access to this content
      Index (pages 961–973)

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