Production of Membrane Proteins: Strategies for Expression and Isolation

Production of Membrane Proteins: Strategies for Expression and Isolation

Editor(s): Anne Skaja Robinson

Published Online: 18 JUN 2011 03:58PM EST

Print ISBN: 9783527327294

Online ISBN: 9783527634521

DOI: 10.1002/9783527634521

About this Book

Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages.
Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations.
Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale.
Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.

Table of contents

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  1. Part One: Expression Systems

    1. Chapter 2

      Membrane Protein Expression in Saccharomyces cerevisiae (pages 37–73)

      Zachary Britton, Carissa Young, Özge Can, Patrick McNeely, Andrea Naranjo and Prof. Anne Skaja Robinson

    2. Chapter 3

      Expression Systems: Pichia pastoris (pages 75–108)

      Fatima Alkhalfioui, Christel Logez, Olivier Bornert and Renaud Wagner

    3. Chapter 4

      Heterologous Production of Active Mammalian G-Protein-Coupled Receptors Using Baculovirus-Infected Insect Cells (pages 109–138)

      Mark Chiu, Brian Estvander, Timothy Esbenshade, Steve Kakavas, Kathy Krueger, Marc Lake and Ana Pereda-Lopez

    4. Chapter 5

      Membrane Protein Expression in Mammalian Cells (pages 139–165)

      Deniz B. Hizal, Erika Ohsfeldt, Sunny Mai and Michael J. Betenbaugh

  2. Part Two: Protein-Specific Considerations

  3. Part Three: Emerging Methods and Approaches

    1. Chapter 12

      Solubilization, Purification, and Characterization of Integral Membrane Proteins (pages 317–360)

      Víctor Lórenz-Fonfría, Alex Perálvarez-Marín, Esteve Padrós and Tzvetana Lazarova

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