Production of Membrane Proteins: Strategies for Expression and Isolation
Copyright © 2011 Wiley-VCH Verlag GmbH & Co. KGaA
Editor(s): Anne Skaja Robinson
Published Online: 18 JUN 2011 03:58PM EST
Print ISBN: 9783527327294
Online ISBN: 9783527634521
About this Book
Designed as a research-level guide to current strategies and methods of membrane protein production on the small to intermediate scale, this practice-oriented book provides detailed, step-by-step laboratory protocols as well as an explanation of the principles behind each method, together with a discussion of its relative advantages and disadvantages.
Following an introductory section on current challenges in membrane protein production, the book goes on to look at expression systems, emerging methods and approaches, and protein specific considerations.
Case studies illustrate how to select or sample the optimal production system for any desired membrane protein, saving both time and money on the laboratory as well as the technical production scale.
Unique in its coverage of "difficult" proteins with large membrane-embedded domains, proteins from extremophiles, peripheral membrane proteins, and protein fragments.