Alternative pre-mRNA Splicing: Theory and Protocols

Alternative pre-mRNA Splicing: Theory and Protocols

Editor(s): Stefan Stamm, Christopher W. J. Smith, Reinhard Lührmann

Published Online: 2 FEB 2012

Print ISBN: 9783527326068

Online ISBN: 9783527636778

DOI: 10.1002/9783527636778

About this Book

This book was written for graduate and medical students, as well as clinicians and postdoctoral researchers. It describes the theory of alternative pre-mRNA splicing in
twelve introductory chapters and then introduces protocols and their theoretical background relevant for experimental research. These 43 practical chapters cover: Basic methods, Detection of splicing events, Analysis of alternative pre-mRNA splicing in vitro and in vivo, Manipulation of splicing events, and Bioinformatic analysis of alternative splicing.
A theoretical introduction and practical guide for molecular biologists, geneticists,clinicians and every researcher interested in alternative splicing.

Website: www.wiley-vch.de/home/splicing

Table of contents

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  1. Part One: Theory

    1. Chapter 1

      Splicing in the RNA World (pages 1–12)

      Emanuele Buratti, Maurizio Romano and Francisco E. Baralle

  2. Part Two: Basic Methods

  3. Part Three: Detection of Splicing Events

    1. Chapter 23

      Monitoring Changes in Plant Alternative Splicing Events (pages 248–259)

      Craig G. Simpson, Naeem Hasan Syed, Sujatha Manthri, John D. Fuller, Monika Maronova, Branislav Kusenda, Maria Kalyna, Andrea Barta and John W.S. Brown

  4. Part Four: Analysis of Splicing In Vitro

    1. Chapter 29

      The Preparation of HeLa Cell Nuclear Extracts (pages 311–319)

      Klaus Hartmuth, Maria A. van Santen, Tanja Rösel, Berthold Kastner and Prof. Dr. Reinhard Lührmann

    2. Chapter 31

      Assembly and Isolation of Spliceosomal Complexes In Vitro (pages 330–340)

      Klaus Hartmuth, Maria A. van Santen, Peter Odenwälder and Prof. Dr. Reinhard Lührmann

    3. Chapter 32

      Analysis of Site-Specific RNA–Protein Interactions (pages 342–356)

      Nathalie Marmier-Gourrier, Audrey Vautrin, Christiane Branlant and Isabelle Behm-Ansmant

    4. Chapter 33

      Immunoprecipitation and Pull-Down of Nuclear Proteins (pages 358–364)

      Natalya Benderska, Chiranthani Sumanasekera and Prof. Dr. Stefan Stamm

  5. Part Five: Analysis of Splicing In Vivo

    1. Chapter 37

      Coupled Promoter Splicing Systems (pages 400–407)

      Manuel J. Mu∼noz, Manuel de la Mata and Alberto R. Kornblihtt

    2. Chapter 38

      Stable Cell Lines with Splicing Reporters (pages 408–414)

      Christian Kroun Damgaard, Søren Lykke-Andersen and Jørgen Kjems

    3. Chapter 39

      Splicing Factor ChIP and ChRIP: Detection of Splicing and Splicing Factors at Genes by Chromatin Immunoprecipitation (pages 416–427)

      Aparna K. Sapra, Fernando Carrillo Oesterreich, Marta Pabis, Imke Listerman, Nicole Bardehle and Karla M. Neugebauer

    4. Chapter 42

      In Vivo Analysis of Plant Intron Splicing (pages 450–462)

      Craig G. Simpson, Michele Liney, Diane Davidson, Dominika Lewandowska, Maria Kalyna, Sean Chapman, Andrea Barta and John W. S. Brown

    1. You have free access to this content
    1. You have free access to this content
    1. You have free access to this content

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