BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity

Copyright © 2011 Wiley-VCH Verlag GmbH & Co. KGaA

BSL3 and BSL4 Agents: Proteomics, Glycomics, and Antigenicity

Editor(s): Jiri Stulik, Rudolf Toman, Patrick Butaye, Robert G. Ulrich

Published Online: 30 SEP 2011 05:11AM EST

Print ISBN: 9783527327805

Online ISBN: 9783527638192

DOI: 10.1002/9783527638192

About this Book

Product Information

About The Product

Unique coverage of proteomic and glycomic approaches to better distinguish highly dangerous pathogens, as well as using these to explore novel treatment and prevention options.
The editors and authors are either part of a specialized European network initiated to develop fast and reliable detection and therapy options, or are associated with the core military research complex of the United States.
With its description of the methods, their advantages and limitations, as well as the principle outcomes, this is a must-have resource for all professionals dealing with BSL3 and/or BSL 4 agents.

Table of contents

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      Front Matter (pages I–XIX)

    1. Chapter 1

      Introduction: Application of Proteomic Technologies for the Analysis of Microbial Infections (pages 1–6)

      Prof. Jiri Stulik and Prof. Patrick Butaye

  3. Part One: Basic Proteomic Methods

    1. Chapter 2

      Separation of Proteins and Peptides (pages 7–27)

      Ludovit Skultety

    2. Chapter 3

      Basic Mass Spectrometric Approaches (pages 29–41)

      Lenka Hernychova and Martin Hubalek

    3. Chapter 4

      Quantitative Mass Spectrometric Approaches (pages 43–53)

      Juraj Lenco and Vojtěch Tambor

    4. Chapter 5

      BN-PAGE of Microbial Protein Complexes (pages 55–66)

      Jiri Dresler and Jana Klimentova

    5. Chapter 6

      Analysis of Francisella Tularensis Glycoproteins (pages 67–78)

      Lucie Balonova and Lenka Hernychova

  4. Part Two: Identification of Proteins and Glycans from Microorganisms as Candidate Molecules for Use in Detection/Diagnosis, Therapy, and Prophylaxis

    1. Chapter 7

      Comparative Proteome Analysis of Strains with Differential Virulence (pages 79–93)

      Martin Hubalek and Ivona Pávková

    2. Chapter 8

      Analysis of Francisella Tularensis Acetonitrile Extracts (pages 95–105)

      Lenka Hernychova, Martin Hubalek and Jana Udrzalova

    3. Chapter 9

      Analysis of Culture Filtrate Proteins of Francisella Tularensis (pages 107–114)

      Klara Konecna, Martin Hubalek and Lenka Hernychova

    4. Chapter 10

      Lipopolysaccharides of Coxiella Burnetii: Chemical Composition and Structure, and their Role in Diagnosis of Q Fever (pages 115–123)

      Prof. Rudolf Toman and Pavol Vadovič

    5. Chapter 11

      Mimivirus Possesses Anonymous and Unique Gene Products Endowed for Antigenic Properties (pages 125–129)

      Patricia Renesto and Didier Raoult

    6. Chapter 12

      Detection of Differentially Modified Pathogen Proteins by Western Blot after 2D Gel Electrophoresis and Identification by MALDI-TOF/TOF (pages 131–138)

      Fred Fack, Julia Kessler, Patrick Pirrotte, Jacques Kremer, Dominique Revets, Wim Ammerlaan and Claude P. Muller

    7. Chapter 13

      Composition and Structure of Lipid A of the Intracellular Bacteria Piscirickettsia Salmonis and Coxiella Burnetii (pages 139–144)

      Pavol Vadovič, Robert Ihnatko and Prof. Rudolf Toman

    8. Chapter 14

      Proteins of Coxiella Burnetii and Analysis of their Function (pages 145–151)

      Robert Ihnatko, Pavol Vadovič and Prof. Rudolf Toman

    9. Chapter 15

      Subtype and Toxin Variant Identification of Botulinum Neurotoxin Type a Using Proteomics Techniques (pages 153–165)

      Suzanne R. Kalb, Jakub Baudys, Theresa J. Smith, James L. Pirkle and John R. Barr

    10. Chapter 16

      Protein Microarrays for Antigen Discovery (pages 167–178)

      Mohan Natesan, Sarah Keasey and Dr. Robert G. Ulrich

    11. Chapter 17

      MALDI-TOF Mass Spectrometry for Rapid Identification of Highly Pathogenic Microorganisms (pages 179–212)

      Peter Lasch and Dieter Naumann

  5. Part Three: Analysis of Host–Pathogen Interactions

    1. Chapter 18

      Quantitative Proteomic Profiling of the Interaction of Francisella Tularensis LVS with Macrophages Using J774.2 Cell Line (pages 213–222)

      Anetta Hartlova, Marek Link, Juraj Lenco and Prof. Jiri Stulik

    2. Chapter 19

      Proteome Analysis of Bacterial Protein Expression after Ingestion of Microbes by Macrophages (pages 223–231)

      Martin Brychta and Ivona Pávková

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      Index (pages 233–236)

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