NMR of Biomolecules: Towards Mechanistic Systems Biology

Copyright © 2012 Wiley-VCH Verlag GmbH & Co. KGaA

NMR of Biomolecules: Towards Mechanistic Systems Biology

Editor(s): Ivano Bertini, Kathleen S. McGreevy, Giacomo Parigi

Published Online: 21 MAR 2012

Print ISBN: 9783527328505

Online ISBN: 9783527644506

DOI: 10.1002/9783527644506

About this Book

Product Information

About The Product

NMR is one of the most powerful methods for imaging of biomolecules. This book is the ultimate NMR guide for researchers in the biomedical community and gives not only background and practical tips but also a forward looking view on the future of NMR in systems biology.

Table of contents

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      Front Matter (pages I–XXXVIII)

  2. Part One: Introduction

    1. Chapter 1

      NMR and its Place in Mechanistic Systems Biology (pages 1–5)

      Prof. Dr. Ivano Bertini, Kathleen S. McGreevy and Prof. Giacomo Parigi

    2. Chapter 2

      Structure of Biomolecules: Fundamentals (pages 7–32)

      Lucia Banci, Francesca Cantini, Mirko Cevec, Hendrik R. A. Jonker, Senada Nozinovic, Christian Richter and Harald Schwalbe

    3. Chapter 3

      What Can be Learned about the Structure and Dynamics of Biomolecules from NMR (pages 33–50)

      Lucio Ferella, Antonio Rosato, Paola Turano and Janez Plavec

  3. Part Two: Role of NMR in the Study of the Structure and Dynamics of Biomolecules

    1. Chapter 4

      Determination of Protein Structure and Dynamics (pages 51–94)

      Lucio Ferella, Antonio Rosato and Paola Turano

    2. Chapter 5

      DNA (pages 96–116)

      Janez Plavec

    3. Chapter 6

      RNA (pages 118–135)

      Richard Štefl and Vladimír Sklenář

    4. Chapter 7

      Intrinsically Disordered Proteins (pages 136–152)

      Isabella C. Felli, Roberta Pierattelli and Peter Tompa

    5. Chapter 8

      Paramagnetic Molecules (pages 154–171)

      Ivano Bertini, Claudio Luchinat and Giacomo Parigi

  4. Part Three: Role of NMR in the Study of the Structure and Dynamics of Biomolecular Interactions

    1. Chapter 9

      NMR Methodologies for the Analysis of Protein–Protein Interactions (pages 173–194)

      Tobias Madl and Michael Sattler

    2. Chapter 10

      Metal-Mediated Interactions (pages 196–203)

      Simone Ciofi-Baffoni

    3. Chapter 11

      Protein–Paramagnetic Protein Interactions (pages 204–217)

      Peter H. J. Keizers, Yoshitaka Hiruma and Marcellus Ubbink

    4. Chapter 12

      Protein–RNA Interactions (pages 218–236)

      Vijayalaxmi Manoharan, Jose Manuel Pérez-Cañadillas and Andres Ramos

    5. Chapter 13

      Protein–DNA Interactions (pages 238–252)

      Lidija Kovačič and Rolf Boelens

  5. Part Four: NMR in Drug Discovery

    1. Chapter 14

      High-Throughput Screening and Fragment-Based Design: General Considerations for Lead Discovery and Optimization (pages 253–263)

      Maurizio Pellecchia

    2. Chapter 15

      Ligand-Observed NMR in Fragment-Based Approaches (pages 264–280)

      Paweł Śledź, Chris Abell and Alessio Ciulli

    3. Chapter 16

      Interactions of Metallodrugs with DNA (pages 282–296)

      Hong-Ke Liu and Peter J. Sadler

    4. Chapter 17

      RNA as a Drug Target (pages 298–313)

      Jan-Peter Ferner, Elke Duchardt Ferner, Jörg Rinnenthal, Janina Buck, Jens Wöhnert and Harald Schwalbe

    5. Chapter 18

      Fluorine NMR Spectroscopy for Biochemical Screening in Drug Discovery (pages 314–327)

      Claudio Dalvit

    6. Chapter 19

      NMR of Peptides (pages 328–344)

      Johannes G. Beck, Andreas O. Frank and Horst Kessler

  6. Part Five: Solid-State NMR

    1. Chapter 20

      Biomolecular Solid-State NMR/Basics (pages 345–364)

      Emeline Barbet-Massin and Guido Pintacuda

    2. Chapter 21

      Protein Dynamics in the Solid State (pages 366–375)

      Józef R. Lewandowski and Lyndon Emsley

    3. Chapter 22

      Microcrystalline Proteins – An Ideal Benchmark for Methodology Development (pages 376–392)

      W. Trent Franks, Barth-Jan van Rossum, Benjamin Bardiaux, Enrico Ravera, Giacomo Parigi, Claudio Luchinat and Hartmut Oschkinat

    4. Chapter 23

      Structural Studies of Protein Fibrils by Solid-State NMR (pages 394–405)

      Anja Böckmann and Beat H. Meier

    5. Chapter 24

      Solid-State NMR on Membrane Proteins: Methods and Applications (pages 406–417)

      A. A. Cukkemane, M. Renault and M. Baldus

  7. Part Six: Frontiers in NMR Spectroscopy

    1. Chapter 25

      Dynamic Nuclear Polarization (pages 419–431)

      Thomas F. Prisner

    2. Chapter 26

      13C Direct Detection NMR (pages 432–443)

      Isabella C. Felli and Roberta Pierattelli

    3. Chapter 27

      Speeding up Multidimensional NMR Data Acquisition (pages 444–465)

      Bernhard Brutscher, Dominique Marion and Lucio Frydman

    4. Chapter 28

      Metabolomics (pages 466–477)

      Leonardo Tenori

    5. Chapter 29

      In-Cell Protein NMR Spectroscopy (pages 478–494)

      David S. Burz, David Cowburn, Kaushik Dutta and Alexander Shekhtman

    6. Chapter 30

      Structural Investigation of Cell-Free Expressed Membrane Proteins (pages 496–508)

      Solmaz Sobhanifar, Sina Reckel, Frank Löhr, Frank Bernhard and Volker Dötsch

  8. Part Seven: Computational Aspects

    1. Chapter 31

      Grid Computing (pages 509–518)

      Antonio Rosato

    2. Chapter 32

      Protein–Protein Docking with HADDOCK (pages 520–535)

      Christophe Schmitz, Adrien S. J. Melquiond, Sjoerd J. de Vries, Ezgi Karaca, Marc van Dijk, Panagiotis L. Kastritis and Alexandre M. J. J. Bonvin

    3. Chapter 33

      Automated Protein Structure Determination Methods (pages 536–546)

      Paul Guerry and Torsten Herrmann

    4. Chapter 34

      NMR Structure Determination of Protein–Ligand Complexes (pages 548–561)

      Ulrich Schieborr, Sridhar Sreeramulu and Harald Schwalbe

    5. Chapter 35

      Small Angle X-Ray Scattering/Small Angle Neutron Scattering as Methods Complementary to NMR (pages 562–574)

      M. V. Petoukhov and D. I. Svergun

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      References (pages 575–608)

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      Index (pages 609–612)

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