Tag-Based Next Generation Sequencing

Tag-Based Next Generation Sequencing

Editor(s): Matthias Harbers, Günter Kahl

Published Online: 23 JAN 2012

Print ISBN: 9783527328192

Online ISBN: 9783527644582

DOI: 10.1002/9783527644582

About this Book

Tag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequencing will benefit from ever more powerful sequencing methods, analytical applications can be performed by tag-based approaches, where the focus shifts from 'sequencing power' to better means of data analysis and visualization for common users. Today Next Generation Sequence data require powerful bioinformatics expertise that has to be converted into easy-to-use data analysis tools. The book's intention is to give an overview on recently developed tag-based approaches along with means of their data analysis together with introductions to Next-Generation Sequencing Methods, protocols and user guides to be an entry for scientists to tag-based approaches for Next Generation Sequencing.

Table of contents

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  1. Part One: Tag-Based Nucleic Acid Analysis

    1. Chapter 13

      Tag-Seq: Next-Generation Tag Sequencing for Gene Expression Profiling (pages 211–241)

      Sorana Morrissy, Yongjun Zhao, Allen Delaney, Jennifer Asano, Noreen Dhalla, Irene Li, Helen McDonald, Pawan Pandoh, Anna-Liisa Prabhu, Angela Tam, Martin Hirst and Marco Marra

    2. Chapter 17

      Next-Generation Sequencing of Bacterial Artificial Chromosome Clones for Next-Generation Physical Mapping (pages 287–298)

      Robert Bogden, Keith Stormo, Jason Dobry, Amy Mraz, Quanzhou Tao, Michiel van Eijk, Jan van Oeveren, Marcel Prins, Jon Wittendorp and Mark van Haaren

  2. Part Two: Next-Generation Tag-Based Sequencing

  3. Part Three: Bioinformatics for Tag-Based Technologies

    1. Chapter 27

      Experimental Design and Quality Control of Next-Generation Sequencing Experiments (pages 417–433)

      Peter A. C. 't Hoen, Matthew S. Hestand, Judith M. Boer, Yuching Lai, Maarten van Iterson, Michiel van Galen, Henk P. Buermans and Johan T. den Dunnen

    2. Chapter 28

      UTGB Toolkit for Personalized Genome Browsers (pages 435–448)

      Taro L. Saito, Jun Yoshimura, Budrul Ahsan, Atsushi Sasaki, Reginaldo Kurosh and Shinichi Morishita

    1. You have free access to this content
    1. You have free access to this content
    1. You have free access to this content