Effect of retinoic acid on prostatic development

Authors

  • Sherif R. Aboseif,

    Corresponding author
    1. Department of Urology, University of California, School of Medicine, San Francisco, California
    • Department of Urology, U-575, University of California, San Francisco, CA 94143-0738
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  • Rajvir Dahiya,

    1. Department of Urology, University of California, School of Medicine, San Francisco, California
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  • Perinchery Narayan,

    1. Department of Urology, University of California, School of Medicine, San Francisco, California
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  • Gerald R. Cunha

    1. Department of Urology, University of California, School of Medicine, San Francisco, California
    2. Department of Developmental Anatomy, University of California, School of Medicine, San Francisco, California
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Abstract

BACKGROUND AND METHODS. To assess the effect of retinoids on prostatic ductal branching morphogenesis, anterior prostates from newborn rats were cultured under serum-free conditions for 6 days in the presence of testosterone (10−8 mM) plus 13-cis-retinoic acid (13-cis-RA), all-trans-retinoic acid (at-RA), or N-4-hydroxyphenyl-retinamide (4-HPR). Measures of morphologic complexity were computed and compared between specimens of different treatment groups.

RESULTS

Prostatic ductal growth and branching were inhibited in a dose-dependent fashion by both 13-cis-RA and at-RA, but not by 4-HPR. This inhibitory effect of 13-cis-RA was reversible, as the prostatic ducts resumed branching and growth after removal of retinoic acid from the culture medium. Using reverse transcription polymerase chain reaction, we then investigated the expression of nuclear receptor genes for retinoic acid.

CONCLUSIONS

This showed the presence of RAR-β and RAR-γ in the 0-day prostate, suggesting that the effects of these retinoids on ductal morphogenesis may be via these receptors. Prostate 31:161–167, 1997. © 1997 Wiley-Liss, Inc.

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