Yeast Functional Analysis Report
A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol
Article first published online: 28 APR 1999
Copyright © 1997 John Wiley & Sons, Ltd.
Volume 13, Issue 14, pages 1347–1355, November 1997
How to Cite
Gonzalez, B., François, J. and Renaud, M. (1997), A rapid and reliable method for metabolite extraction in yeast using boiling buffered ethanol. Yeast, 13: 1347–1355. doi: 10.1002/(SICI)1097-0061(199711)13:14<1347::AID-YEA176>3.0.CO;2-O
- Issue published online: 28 APR 1999
- Article first published online: 28 APR 1999
- Manuscript Accepted: 16 APR 1997
- Manuscript Received: 18 FEB 1997
- French Ministry of Education
- 1991. Toward a science of metabolic engineering. Science 252, 1668–1675.
- 1985. Methods in Enzymatic Analysis, vol. VI and VII. VCH Publishers.
- 1993. Different signals control activation of glycolysis in the yeast Saccharomyces cerevisiae. Yeast 9, 761–770. and
- 1979. Physiological effects of seven different blocks in glycolysis in Saccharomyces cerevisiae. J. Bacteriol. 139, 152–160. and
- 1992. Continuous-flow NMR bioreactor for in vivo studies of microbial cell suspensions with low biomass concentration. J. Magn. Res. 98, 654–659. , , , , and
- 1992. A method for the determination of changes of glycolytic metabolites in yeast on a subsecond time scale using extraction at neutral pH. Anal. Biochem. 204, 118–123. and
- 1977. A partial defect in carbon catabolite repression mutants of Saccharomyces cerevisiae with reduced hexose phosphorylation. Mol. Gen. Genet. 156, 99–105. , and
- 1984. Effect of nitrogen and phosphate on the levels of intermediates in baker's yeast grown in continuous culture. J. Gen. Microbiol. 130, 2465–2472. , and
- 1984. The mechanism by which glucose increases fructose-2,6-bisphosphate concentration in Saccharomyces cerevisiae. Eur. J. Biochem. 145, 187–193. , and
- 1993. Characterization of mutations that overcome the toxic effect of glucose on phosphoglucose isomerase less strains of Saccharomyces cerevisiae. FEMS Microbiol. 106, 233–238. , and
- 1973. Concentrations of intermediary metabolites in yeast. Biochimie 55, 205–211. and
- 1996. Development and application of a membrane cyclone reactor for in vivoNMR spectroscopy with high microbial cell densities. Biotechnol. Bioeng. 51, 624–635. , , , and
- 1974. In Bergmeyer, H. U. ( Ed.), Methods of Enzymatic Analysis. Academic Press, New York, pp. 2102–2110.
- 1996. Determination of intermediary metabolites in Aspergillus niger. J. Microbiol. Methods 25, 295–302. and
- 1993. Metabolic design. In and Reed, G. ( Eds), Biotechnology, 1. VCH Weinheim, pp. 190–221.
- 1993. In vivo analysis of glucose-induced fast glycolytic changes in yeast adenine nucleotide pool applying a rapid sampling technique. Anal. Biochem. 214, 31–37. , , and
- 1992. Effect of benzoic acid on metabolite fluxes in yeasts: A continuous-culture study on the regulation of respiration and alcoholic fermentation. Yeast 8, 501–517. , , and
- 1974. Rapid sampling of yeast cells and automated assays of adenylate, citrate, pyruvate and glucose-6-phosphate pools. Anal. Biochem. 58, 208–216. , and
- 1996. What Bio-Technologists knew all along…? J. Theor. Biol. 182, 411–420. and
- 1996. Reaction engineering methods to study intracellular metabolite concentrations. Adv. Biochem. Eng. Biotech. 34, 75–110. and