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Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications

  1. Carrie Baker Brachmann,
  2. Adrian Davies,
  3. Gregory J. Cost,
  4. Emerita Caputo,
  5. Joachim Li†,
  6. Philip Hieter,
  7. Jef D. Boeke*

Article first published online: 4 DEC 1998

DOI: 10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2

Issue

Yeast

Yeast

Volume 14, Issue 2, pages 115–132, 30 January 1998

Additional Information

How to Cite

Baker Brachmann, C., Davies, A., Cost, G. J., Caputo, E., Li, J., Hieter, P. and Boeke, J. D. (1998), Designer deletion strains derived from Saccharomyces cerevisiae S288C: A useful set of strains and plasmids for PCR-mediated gene disruption and other applications. Yeast, 14: 115–132. doi: 10.1002/(SICI)1097-0061(19980130)14:2<115::AID-YEA204>3.0.CO;2-2

Author Information

  1. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, MD 21205, U.S.A.

  1. †

    Department of Biochemistry, University of California, San Francisco, CA, U.S.A.

*Hunterian 617, Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, 725 N. Wolfe Street, Baltimore, MD 21205, U.S.A. Tel: (+1) 410 955 2481; fax: (+1) 410 614 2987

Publication History

  1. Issue published online: 4 DEC 1998
  2. Article first published online: 4 DEC 1998
  3. Manuscript Accepted: 18 JUN 1997
  4. Manuscript Revised: 9 JUN 1997

Funded by

  • NIH

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Keywords:

  • Saccharomyces cerevisiae;
  • yeast;
  • gene disruption;
  • S288C;
  • bacteria-yeast shuttle vectors;
  • auxotrophic markers

Abstract

A set of yeast strains based on Saccharomyces cerevisiae S288C in which commonly used selectable marker genes are deleted by design based on the yeast genome sequence has been constructed and analysed. These strains minimize or eliminate the homology to the corresponding marker genes in commonly used vectors without significantly affecting adjacent gene expression. Because the homology between commonly used auxotrophic marker gene segments and genomic sequences has been largely or completely abolished, these strains will also reduce plasmid integration events which can interfere with a wide variety of molecular genetic applications. We also report the construction of new members of the pRS400 series of vectors, containing the kanMX, ADE2 and MET15 genes. © 1998 John Wiley & Sons, Ltd.

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