A search in the genome of Saccharomyces cerevisiae for genes regulated via stress response elements

Authors

  • E. Moskvina,

    1. Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Dr Bohrgasse 9, A-1030 Wien, Austria
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  • C. Schüller,

    1. Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Dr Bohrgasse 9, A-1030 Wien, Austria
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  • C. T. C. Maurer,

    1. Department of Biochemistry and Molecular Biology, JMBW, Biocentrum Amsterdam, Vrije Universiteit, De Boelaan 1083, 1081 HV Amsterdam, The Netherlands
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  • W. H. Mager,

    1. Department of Biochemistry and Molecular Biology, JMBW, Biocentrum Amsterdam, Vrije Universiteit, De Boelaan 1083, 1081 HV Amsterdam, The Netherlands
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  • H. Ruis

    Corresponding author
    1. Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie der Universität Wien and Ludwig Boltzmann-Forschungsstelle für Biochemie, Dr Bohrgasse 9, A-1030 Wien, Austria
    • Vienna Biocenter, Institut für Biochemie und Molekulare Zellbiologie, Dr Bohrgasse 9, A-1030 Wien, Austria. Tel.: (+43) 1 79515 5115; fax: (+43) 1 7995272
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Abstract

Stress response elements (STREs, core consensus AG4 or C4T) have been demonstrated previously to occur in the upstream region of a number of genes responsive to induction by a variety of stress signals. This stress response is mediated by the homologous transcription factors Msn2p and Msn4p, which bind specifically to STREs. Double mutants (msn2 msn4) deficient in these transcription factors have been shown to be hypersensitive to severe stress conditions. To obtain a more representative overview of the set of yeast genes controlled via this regulon, a computer search of the Saccharomyces cerevisiae genome was carried out for genes, which, similar to most known STRE-controlled genes, exhibit at least two STREs in their upstream region. In addition to the great majority of genes previously known to be controlled via STREs, 69 open reading-frames were detected. Expression patterns of a set of these were examined by grid filter hybridization, and 14 genes were examined by Northern analysis. Comparison of the expression patterns of these genes demonstrates that they are all STRE-controlled although their detailed expression patterns differ considerably. © 1998 John Wiley & Sons, Ltd.

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