Dynamic responses of reserve carbohydrate metabolism under carbon and nitrogen limitations in Saccharomyces cerevisiae

Authors

  • Jean-Luc Parrou,

    1. Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France
    Search for more papers by this author
  • Brice Enjalbert,

    1. Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France
    Search for more papers by this author
  • Lucile Plourde,

    1. Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France
    Search for more papers by this author
  • Anne Bauche,

    1. Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France
    Search for more papers by this author
  • Benjamin Gonzalez,

    1. Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France
    Search for more papers by this author
  • Jean François

    Corresponding author
    1. Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France
    • Centre de Bioingenierie Gilbert Durand, UMR-CNRS 5504, LA. INRA, INSA, Complexe Scientifique de Rangueil, 31077 Toulouse Cedex 04, France.
    Search for more papers by this author

Abstract

The dynamic responses of reserve carbohydrates with respect to shortage of either carbon or nitrogen source was studied to obtain a sound basis for further investigations devoted to the characterization of mechanisms by which the yeast Saccharomyces cerevisiae can cope with nutrient limitation during growth. This study was carried out in well-controlled bioreactors which allow accurate monitoring of growth and frequent sampling without disturbing the culture. Under glucose limitation, genes involved in glycogen and trehalose biosynthesis (GLG1, GSY1, GSY2, GAC1, GLC3, TPS1 ), in their degradation (GPH1, NTH1 ), and the typical stress-responsive CTT1 gene were coordinately induced in parallel with glycogen, when the growth has left the pure exponential phase and while glucose was still plentiful in the medium. Trehalose accumulation was delayed until the diauxic shift, although TPS1 was induced much earlier, due to hydrolysis of trehalose by high trehalase activity. In contrast, under nitrogen limitation, both glycogen and trehalose began to accumulate at the precise time when the nitrogen source was exhausted from the medium, coincidentally with the transcriptional activation of genes involved in their metabolism. While this response to nitrogen starvation was likely mediated by the stress-responsive elements (STREs) in the promoter of these genes, we found that these elements were not responsible for the co-induction of genes involved in reserve carbohydrate metabolism during glucose limitation, since GLG1, which does not contain any STRE, was coordinately induced with GSY2 and TPS1. Copyright © 1999 John Wiley & Sons, Ltd.

Ancillary