Two populations of β-spectrin in rat skeletal muscle
Article first published online: 6 DEC 1998
Copyright © 1997 Wiley-Liss, Inc.
Cell Motility and the Cytoskeleton
Volume 37, Issue 1, pages 7–19, 1997
How to Cite
Porter, G. A., Scher, M. G., Resneck, W. G., Porter, N. C., Fowler, V. M. and Bloch, R. J. (1997), Two populations of β-spectrin in rat skeletal muscle. Cell Motil. Cytoskeleton, 37: 7–19. doi: 10.1002/(SICI)1097-0169(1997)37:1<7::AID-CM2>3.0.CO;2-7
- Issue published online: 6 DEC 1998
- Article first published online: 6 DEC 1998
- Manuscript Accepted: 14 JAN 1997
- Manuscript Received: 24 JUN 1996
- NIH. Grant Numbers: GM34225, NS17282, HD16596, GM08181
- Muscular Dystrophy Assoc
- membrane skeleton;
- skeletal muscle
We use immunoblotting, immunoprecipitation, and centrifugation in sucrose density gradients to show that the product of the erythrocyte β-spectrin gene in rat skeletal muscle (muscle β-spectrin) is present in two states, one associated with fodrin, and another that is not associated with any identifiable spectrin or fodrin subunit. Immunofluorescence studies indicate that a significant amount of β-spectrin without α-fodrin is present in the myoplasm of some muscle fibers, and, more strikingly, at distinct regions of the sarcolemma. These results suggest that α-fodrin and muscle β-spectrin associate in muscle in situ, but that some muscle β-spectrin without a paired α-subunit forms distinct domains at the sarcolemma. Cell Motil. Cytoskeleton 37:7–19, 1997. © 1997 Wiley-Liss, Inc.