Expression of a novel type of classic cadherin, PB-cadherin in developing brain and limb buds
Article first published online: 16 SEP 1999
Copyright © 1999 Wiley-Liss, Inc.
Volume 215, Issue 3, pages 206–214, July 1999
How to Cite
Kitajima, K., Koshimizu, U. and Nakamura, T. (1999), Expression of a novel type of classic cadherin, PB-cadherin in developing brain and limb buds. Dev. Dyn., 215: 206–214. doi: 10.1002/(SICI)1097-0177(199907)215:3<206::AID-AJA3>3.0.CO;2-X
- Issue published online: 16 SEP 1999
- Article first published online: 16 SEP 1999
- Manuscript Accepted: 14 APR 1999
- Manuscript Received: 21 DEC 1998
- neural tissues;
- segment boundary;
- limb bud;
- anterior-posterior axis;
PB-cadherin is a novel classic type of cadherin predominantly expressed in brain of adult rats (Sugimoto et al.  J. Biol. Chem. 271:11548–11556). To examine the spatial and temporal expression of PB-cadherin during development, we isolated full-length cDNA of mouse PB-cadherin and studied its expression pattern in mouse embryos. In Northern blots, PB-cadherin mRNA was detected at 9.5 days postcoitum (dpc) onwards. Whole-mount in situ hybridization showed that PB-cadherin signals mainly occurred in developing neural tissues, including brain and spinal cord, and limb buds in the 10.5 dpc embryo. In the brain, PB-cadherin mRNA were strongly expressed in the forebrain and midbrain-hindbrain boundary region (isthmus). In isthmus, PB-cadherin expression delineated the expression area of Wnt-1, a secreted signaling molecule essential for proper cerebellum development. In the developing limb, PB-cadherin mRNA was first localized in posterior part of buds at 10.5 dpc, and was thereafter distributed in a domain around the digit rudiments. This expression pattern is similar to that of BMP-2, a secreted signalling molecule involving limb patterning and morphogenesis. These findings suggested the possibility that PB-cadherin-mediated cell-cell adhesion has a functional role in pattern formation and morphogenesis of mouse embryonic brain and limb. Dev Dyn 1999;215:206–214. © 1999 Wiley-Liss, Inc.