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YAC transgene-mediated olfactory receptor gene choice

  1. Farah A.W. Ebrahimi1,2,
  2. James Edmondson3,
  3. Rodney Rothstein4,
  4. Andrew Chess1,2,*

Article first published online: 15 MAR 2000

DOI: 10.1002/(SICI)1097-0177(200002)217:2<225::AID-DVDY9>3.0.CO;2-3

Issue

Developmental Dynamics

Developmental Dynamics

Volume 217, Issue 2, pages 225–231, February 2000

Additional Information

How to Cite

Ebrahimi, F. A., Edmondson, J., Rothstein, R. and Chess, A. (2000), YAC transgene-mediated olfactory receptor gene choice. Developmental Dynamics, 217: 225–231. doi: 10.1002/(SICI)1097-0177(200002)217:2<225::AID-DVDY9>3.0.CO;2-3

Author Information

  1. 1

    Whitehead Institute for Biomedical Research, Cambridge, Massachusetts

  2. 2

    Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts

  3. 3

    Department of Neurology, College of Physicians and Surgeons, Columbia University, New York, New York

  4. 4

    Department of Genetics and Development, College of Physicians and Surgeons, Columbia University, New York, New York

*Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142

Publication History

  1. Issue published online: 15 MAR 2000
  2. Article first published online: 15 MAR 2000
  3. Manuscript Accepted: 22 OCT 1999
  4. Manuscript Received: 9 SEP 1999

Funded by

  • National Institutes of Health/The National Institute of Deafness and other Communicative Disorders. Grant Numbers: R01DC03263, R03DC04035

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Keywords:

  • transgenic mice;
  • olfactory receptor;
  • olfaction;
  • vomeronasal organ;
  • olfactory epithelium;
  • allelic inactivation;
  • gene expression

Abstract

In the mouse, individual olfactory neurons express one of a thousand distinct olfactory receptor genes. Furthermore, only one allele of the expressed gene is transcribed. This phenomenon, random allelic inactivation, along with the observation that the olfactory receptor genes reside in large chromosomal arrays, suggests a role for long-range gene regulation in olfactory receptor gene choice. We have constructed a 300-kb yeast artificial chromosome (YAC) transgene in which a single receptor gene is marked while maintaining its coding region. This 300-kb piece of DNA functions as an independent olfactory receptor gene locus in directing olfactory receptor gene choice in both the olfactory system and the accessory olfactory system (vomeronasal organ, VNO). Furthermore, the transgene, like endogenous olfactory receptor loci, is subject to allelic inactivation. Dev Dyn;217:225–231. © 2000 Wiley-Liss, Inc.

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