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A fragment of the neurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo

  1. Richard C. Murray1,
  2. Stephen J. Tapscott2,3,
  3. Jean W. Petersen4,
  4. Anne L. Calof1,*,
  5. Mary B. Mccormick4,†

Article first published online: 18 MAY 2000

DOI: 10.1002/(SICI)1097-0177(200005)218:1<189::AID-DVDY16>3.0.CO;2-4

Issue

Developmental Dynamics

Developmental Dynamics

Volume 218, Issue 1, pages 189–194, May 2000

Additional Information

How to Cite

Murray, R. C., Tapscott, S. J., Petersen, J. W., Calof, A. L. and Mccormick, M. B. (2000), A fragment of the neurogenin1 gene confers regulated expression of a reporter gene in vitro and in vivo. Developmental Dynamics, 218: 189–194. doi: 10.1002/(SICI)1097-0177(200005)218:1<189::AID-DVDY16>3.0.CO;2-4

Author Information

  1. 1

    Department of Anatomy and Neurobiology and the Developmental Biology Center, University of California, Irvine, College of Medicine, Irvine, California

  2. 2

    Fred Hutchinson Cancer Research Center, Seattle, Washington

  3. 3

    Department of Neurology, University of Washington Medical Center, Seattle, Washington

  4. 4

    Department of Medical Genetics, University of Wisconsin-Madison, Madison, Wisconsin

  1. †

    Cancer Biology Program, Beth Israel Deaconess, Harvard Medical School, Boston, MA 02215

*Department of Anatomy and Neurobiology and the Developmental Biology Center, 364 Med Surge II, UCI College of Medicine, Irvine, CA 92697-1275

Publication History

  1. Issue published online: 18 MAY 2000
  2. Article first published online: 18 MAY 2000
  3. Manuscript Accepted: 24 JAN 2000
  4. Manuscript Received: 1 DEC 1999

Funded by

  • National Institutes of Health. Grant Numbers: DC03583, NS36086
  • March of Dimes Birth Defects Foundation. Grant Number: FY99-424

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Keywords:

  • neurogenin1;
  • transgenic mice;
  • P19 cells;
  • neuronal precursor;
  • neurogenesis;
  • promoter;
  • transcription factor;
  • peripheral nervous system;
  • central nervous system

Abstract

The basic helix-loop-helix transcription factor neurogenin1 is required for proper nervous system development in vertebrates. It is expressed in neuronal precursors during embryonic development and is thought to play a role in specifying neuronal fate. To investigate the regulation of neurogenin1 expression, the transcriptional start site of the gene was identified and a 2.7-kb fragment ending in the first exon was shown to possess basal promoter activity. This 2.7-kb fragment was able to promote expression of reporter genes in P19 cells under conditions in which expression of endogenous neurogenin1 was induced. Importantly, the 2.7-kb fragment was able to drive expression of a lacZ reporter gene in transgenic mice in most tissues in which neurogenin1 is normally expressed, including those peripheral ganglia that fail to develop in neurogenin1 “knockout” mice. These findings identify a regulatory region containing elements responsible for appropriate expression of a gene with a crucial role in generating the vertebrate nervous system. Dev Dyn;218:189–194. © 2000 Wiley-Liss, Inc.

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