Rapid Identification of Intact Whole Bacteria Based on Spectral Patterns using Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry
Article first published online: 6 MAY 1999
Copyright © 1996 John Wiley & Sons, Ltd.
Rapid Communications in Mass Spectrometry
Volume 10, Issue 10, pages 1227–1232, 1996
How to Cite
Holland, R. D., Wilkes, J. G., Rafii, F., Sutherland, J. B., Persons, C. C., Voorhees, K. J. and Lay, J. O. (1996), Rapid Identification of Intact Whole Bacteria Based on Spectral Patterns using Matrix-assisted Laser Desorption/Ionization with Time-of-flight Mass Spectrometry. Rapid Commun. Mass Spectrom., 10: 1227–1232. doi: 10.1002/(SICI)1097-0231(19960731)10:10<1227::AID-RCM659>3.0.CO;2-6
- Issue published online: 6 MAY 1999
- Article first published online: 6 MAY 1999
- Manuscript Accepted: 19 JUN 1996
- Manuscript Received: 7 JUN 1996
Matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) was investigated as a method for the rapid identification of whole bacteria, either by comparison with archived reference spectra or by co-analysis with cultures of known bacteria. Bacteria were sampled from colonies on an agar plate, mixed with the matrix, air-dried, and introduced in batches into the mass spectrometer for analysis. In the first experiment, both bacterial strains that had been previously analyzed to obtain reference spectra and other strains that had not been analyzed were blind-numbered and their spectra were obtained. Those strains that matched reference spectra were found to be correctly identified. A second experiment involved co-analysis of reference strains and bind-numbered strains under identical conditions; species-specific identification was demonstrated by comparison of spectra of the blind-numbered strains with those of the standards. In all of the spectra obtained in these experiments, each bacterial strain showed a few characteristic high-mass ions which are thought to be derived from bacterial proteins. This work represents the first reported instance of successful bacterial chemotaxonomy by MALDI-TOFMS analysis of whole cells. For the strains tested, the method is rapid and simple.