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Research Article
Study of erythromycin A decomposition products in aqueous solution by solid-phase microextraction/liquid chromatography/tandem mass spectrometry
Article first published online: 4 DEC 1998
DOI: 10.1002/(SICI)1097-0231(19980214)12:3<123::AID-RCM126>3.0.CO;2-4
Copyright © 1998 John Wiley & Sons, Ltd.
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Additional Information
How to Cite
Volmer, D. A. and Hui, J. P. M. (1998), Study of erythromycin A decomposition products in aqueous solution by solid-phase microextraction/liquid chromatography/tandem mass spectrometry. Rapid Commun. Mass Spectrom., 12: 123–129. doi: 10.1002/(SICI)1097-0231(19980214)12:3<123::AID-RCM126>3.0.CO;2-4
Publication History
- Issue published online: 4 DEC 1998
- Article first published online: 4 DEC 1998
- Manuscript Accepted: 26 NOV 1997
- Manuscript Revised: 25 NOV 1997
- Manuscript Received: 18 NOV 1997
- Abstract
- Cited By
Abstract
The decomposition of erythromycin A (EA) in aqueous solution was examined in the pH range 2–13 by means of combined solid-phase microextraction (SPME) and liquid chromatography/electrospray ionization tandem mass spectrometry. Degradation of EA, especially at lower pH values (pH ≤ 3), was very rapid and yielded a wide variety of decomposition products. Identification of these degradation products was achieved by means of tandem mass spectrometry in the product ion and precursor ion scan modes. Anhydroerythromycin A was shown to be the major reaction product in both acidic and basic solutions. Among the different SPME fibers investigated for extraction from aqueous solutions, polydimethylsiloxane/divinylbenzene exhibited the best performance for EA and its degradation products. © 1998 John Wiley & Sons, Ltd.