Medium optimization for spore production of Coniothyrium minitans using statistically-based experimental designs

  1. L. P. Ooijkaas1,*,
  2. E. C. Wilkinson1,
  3. J. Tramper2,
  4. R. M. Buitelaar1

Article first published online: 26 MAR 2000

DOI: 10.1002/(SICI)1097-0290(19990705)64:1<92::AID-BIT10>3.0.CO;2-8

Issue

Biotechnology and Bioengineering

Biotechnology and Bioengineering

Volume 64, Issue 1, pages 92–100, 5 July 1999

Additional Information

How to Cite

Ooijkaas, L. P., Wilkinson, E. C., Tramper, J. and Buitelaar, R. M. (1999), Medium optimization for spore production of Coniothyrium minitans using statistically-based experimental designs. Biotechnol. Bioeng., 64: 92–100. doi: 10.1002/(SICI)1097-0290(19990705)64:1<92::AID-BIT10>3.0.CO;2-8

Author Information

  1. 1

    Agrotechnological Research Institute (ATO-DLO), Industrial Agrobiotechnology, P.O. Box 17, 6700 AA Wageningen, The Netherlands

  2. 2

    Food and Bioprocess Engineering Group, Wageningen Agricultural University, P.O. Box 8129, 6700 EV Wageningen, The Netherlands

*Agrotechnological Research Institute (ATO-DLO), Industrial Agrobiotechnology, P.O. Box 17, 6700 AA Wageningen, The Netherlands

Publication History

  1. Issue published online: 26 MAR 2000
  2. Article first published online: 26 MAR 2000
  3. Manuscript Accepted: 2 DEC 1998
  4. Manuscript Received: 22 JAN 1998

Funded by

  • Dutch Technology Foundation (STW). Grant Number: WBI44.3318

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Keywords:

  • Coniothyrium minitans;
  • spore production;
  • solid-state fermentation;
  • experimental designs;
  • response surface;
  • medium optimization;
  • fungi

Abstract

Statistically-based experimental designs were used to optimize a chemically defined solid medium for the spore production of Coniothyrium minitans. In the first optimization step the influence of starch, urea, phosphate, magnesium, calcium, thiamin and trace elements on spore production was evaluated using a fractional factorial design. Starch and trace elements influenced spore production positively while urea affected spore production negatively. The other components had no significant influence on spore production. In the second and third steps the concentrations of starch, urea and trace elements were further optimized using central composite designs and response surface analysis. This optimization strategy allowed the spore production to be increased by a factor 7 from 4 × 109 to almost 3 × 1010 spores per Petri dish of 9 cm diameter. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 92–100, 1999.

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