Detection of HIV-RNA-positive monocytes in peripheral blood of HIV-positive patients by simultaneous flow cytometric analysis of intracellular HIV RNA and cellular immunophenotype
Article first published online: 6 DEC 1998
Copyright © 1998 Wiley-Liss, Inc.
Volume 31, Issue 4, pages 265–274, 1 April 1998
How to Cite
Patterson, B. K., Mosiman, V. L., Cantarero, L., Furtado, M., Bhattacharya, M. and Goolsby, C. (1998), Detection of HIV-RNA-positive monocytes in peripheral blood of HIV-positive patients by simultaneous flow cytometric analysis of intracellular HIV RNA and cellular immunophenotype. Cytometry, 31: 265–274. doi: 10.1002/(SICI)1097-0320(19980401)31:4<265::AID-CYTO6>3.0.CO;2-I
- Issue published online: 6 DEC 1998
- Article first published online: 6 DEC 1998
- Manuscript Accepted: 5 DEC 1997
- Manuscript Received: 5 MAY 1997
- Northwestern University Comprehensive AIDS Center
- in situ hybridization;
- in situ PCR;
Determinations of plasma HIV viral RNA copy numbers help to define the kinetics of HIV-1 infection in vivo and to monitor antiretroviral therapy. However, questions remain regarding the identity of various infected cell types contributing to this free virus pool and to the in vivo lifecycle of HIV during disease progression. Characterization of a novel fluorescence in situ hybridization (FISH) assay employing a pool of labeled oligonucleotide probes directed against HIV RNA was done followed by coupling of the FISH assay with simultaneous surface immunophenotyping to address these questions. In vitro characterizations of this assay using tumor necrosis factor-α stimulated and unstimulated ACH-2 cells demonstrated the ability to detect <5% HIV RNA positive cells with a sensitivity of <30 RNA copies per cell. Peripheral blood mononuclear cells from 39 HIV-seropositive patients on no, single, combination, or triple drug therapy and 8 HIV-seronegative patients were examined. The majority of HIV-positive patients (24/39) harbored monocytes positive for HIV RNA and a significantly higher fraction of patients with high plasma viral load carried positive monocytes (13/16) than did patients in the low plasma viral load group (11/23). These results demonstrate the effectiveness of a novel FISH assay for identifying and monitoring HIV-infected cell populations in the peripheral blood of HIV-positive patients. In addition, monocytes are a major source of cellular HIV virus in the peripheral blood of HIV patients, even with progression of disease. Cytometry 31:265–274, 1998. © 1998 Wiley-Liss, Inc.