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Oct-6 (SCIP Tst-1) is expressed in Schwann cell precursors, embryonic Schwann cells, and postnatal myelinating Schwann cells: Comparison with Oct-1, Krox-20, and Pax-3

  1. Andrew D. Blanchard1,
  2. Andrea Sinanan1,
  3. Eric Parmantier1,
  4. Ronald Zwart2,
  5. Ludo Broos2,
  6. Dies Meijer2,
  7. Carola Meier1,
  8. Kristján R. Jessen1,
  9. Rhona Mirsky1,*

Article first published online: 7 DEC 1998

DOI: 10.1002/(SICI)1097-4547(19961201)46:5<630::AID-JNR11>3.0.CO;2-0

Issue

Journal of Neuroscience Research

Journal of Neuroscience Research

Volume 46, Issue 5, pages 630–640, 1 December 1996

Additional Information

How to Cite

Blanchard, A. D., Sinanan, A., Parmantier, E., Zwart, R., Broos, L., Meijer, D., Meier, C., Jessen, K. R. and Mirsky, R. (1996), Oct-6 (SCIP Tst-1) is expressed in Schwann cell precursors, embryonic Schwann cells, and postnatal myelinating Schwann cells: Comparison with Oct-1, Krox-20, and Pax-3. Journal of Neuroscience Research, 46: 630–640. doi: 10.1002/(SICI)1097-4547(19961201)46:5<630::AID-JNR11>3.0.CO;2-0

Author Information

  1. 1

    Department of Anatomy and Developmental Biology, University College London, London England

  2. 2

    Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands

*Department of Anatomy and Developmental Biology, University College London, Gower Street, London WC1E 6BT, England

Publication History

  1. Issue published online: 7 DEC 1998
  2. Article first published online: 7 DEC 1998
  3. Manuscript Revised: 26 AUG 1996
  4. Manuscript Accepted: 26 AUG 1996
  5. Manuscript Received: 2 AUG 1996

Funded by

  • WELLCOME TRUST
  • MEDICAL RESEARCH COUNCIL OF GREAT BRITAIN
  • EUROPEAN COMMUNITY BIOMED 1 PROGRAMME

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Keywords:

  • POU proteins;
  • transcription factors;
  • peripheral nervous system;
  • myelin proteins;
  • reverse transcriptase-polymerase chain reaction

Abstract

The POU domain transcription factor Oct-6 (SCIP/ Tst-1) is likely to control important stages of Schwann cell development, including the initiation of myelination around birth. Here, we use immunocytochemical and reverse transcriptase-polymerase chain reaction techniques to examine Oct-6 earlier in nerve development, to test the idea that Oct-6 has an additional role in Schwann cell precursors or early embryonic Schwann cells, a possibility raised by previous studies on transgenic mice. Consistent with this, we find low but unambiguous levels of Oct-6 mRNA and protein in Schwann cell precursors of mouse and rat (nerves from 12- and 14-day-old embryos, respectively), with expression levels gradually increasing during early Schwann cell development and towards birth. Unexpectedly, Oct-6 immunoreactivity is clearly present in nuclei of most myelinating cells at least as late as postnatal day 12. Furthermore, many nonmyelinating Schwann cells express Oct-6 in adult life. A comparison of Oct-6 mRNA with other Schwann cell transcription factors—namely, Oct-1, Krox-20, and Pax-3—reveals that each factor exhibits strong developmental regulation and a unique expression pattern in embryonic nerves. Therefore, they are likely to play distinct regulatory roles in early development of the Schwann cell lineage. © 1996 Wiley-Liss, Inc.

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